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| Format: | Artículo científico |
| Language: | en |
| Published: |
Universidad de Costa Rica
2009
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| Online Access: | https://www.redalyc.org/articulo.oa?id=44918950013 |
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Table of Contents:
- Histology of somatic embryogenesis in rice (Oryza sativa cv. 5272) Rafael Vega Nelly Vásquez Ana M. Espinoza Andrés M. Gatica Marta Valdez-Melara Biología rice histology Costa Rica Oryza sativa morphogenesis Rice (Oryza sativa cv. 5272) embryogenic calli were obtained from mature zygotic embryos culture on Murashige & Skoog (1962) medium supplemented with 2.5 mg/l 2,4- dichlorophenoxyacetic acid. Histological analysis of somatic embryogenesis revealed that after two weeks of culture of explants on the callus induction medium, somatic embryo development began with a cluster of proembryogenic cells in the peripheral region of the calli. The outer cell layer of embryogenic calli consisted of small and isodiametric cells with a dense cytoplasm and a prominent nucleus and nucleolus; whereas the inner cell layer is composed of large cells with small nucleus and large vacuole. These embryogenic cells underwent a series of organized divisions and formed the proembryo with a well-defined protodermis. 2009 artículo científico 0034-7744 https://www.redalyc.org/articulo.oa?id=44918950013 en http://www.redalyc.org/revista.oa?id=449 Revista de Biología Tropical application/pdf Universidad de Costa Rica Revista de Biología Tropical (Costa Rica) Num.1 Vol.57