Isolation and characterization of infectious Vibrio sinaloensis strains from the Pacific shrimp Litopenaeus vannamei (Decapoda: Penaeidae)
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| Formato: | Artículo científico |
| Lenguaje: | en |
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Universidad de Costa Rica
2012
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| _version_ | 1876475843772940288 |
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| author | Ma. del Carmen Flores-Miranda |
| author_facet | Ma. del Carmen Flores-Miranda |
| contents | Isolation and characterization of infectious Vibrio sinaloensis strains from the Pacific shrimp Litopenaeus vannamei (Decapoda: Penaeidae) Ma. del Carmen Flores-Miranda Antonio Luna-González Ángel I. Campa Córdova Jesús A. Fierro-Coronado Blanca O. Partida-Arangure José Pintado Héctor A. González-Ocampo Biología vibriosis Vibrio sinaloensis enzymatic activity hemolytic activity Litopenaeus vannamei Infectious diseases especially those caused by bacterial and viral pathogens are serious loss factors in shrimp farming. In this study, bacteria were isolated from the gut and hepatopancreas of stressed shrimps obtained from a commercial farm. The isolates were screened on Thiosulfate citrate bile salt sucrose (TCBS) agar plates for the selection of Vibrio species. Presumptive vibrios were characterized through tests for hemolytic and enzymatic activity, hydrophobicity, growth and molecular identification. Three experimental infections were conducted in order to confirm the pathogenicity of selected bacterial strains VHPC18, VHPC23, VHPC24 and VIC30. In the third experimental challenge the LD50 was obtained, it lasted 10 days with 10 shrimp, weighing 6.9±1.1g, per tank. The treatments in triplicate were: (1) saline solution (control group); (2) 2×105CFU/shrimp; (3) 4×105CFU/shrimp; (4) 2×106CFU/shrimp; (5) 4×106CFU/shrimp, and (6) 8×106CFU/shrimp. In all challenges, water parameters measured during the experimental period remained within optimum ranges. Pathogenicity tests confirmed that the mixture of four vibrio isolates, identified as Vibrio sinaloensis, was virulent for L. vannamei. The LD50 value was 1.178×105CFU/g body weight. V. sinaloensis may act as opportunistic pathogens for cultured L. vannamei. 2012 artículo científico 0034-7744 https://www.redalyc.org/articulo.oa?id=44923872005 en http://www.redalyc.org/revista.oa?id=449 Revista de Biología Tropical application/pdf Universidad de Costa Rica Revista de Biología Tropical (Costa Rica) Num.2 Vol.60 |
| format | Artículo científico |
| id | redalyc_44923872005 |
| institution | Redalyc |
| language | en |
| publishDate | 2012 |
| publisher | Universidad de Costa Rica |
| spellingShingle | Isolation and characterization of infectious Vibrio sinaloensis strains from the Pacific shrimp Litopenaeus vannamei (Decapoda: Penaeidae) Ma. del Carmen Flores-Miranda Biología vibriosis Vibrio sinaloensis enzymatic activity hemolytic activity Litopenaeus vannamei Isolation and characterization of infectious Vibrio sinaloensis strains from the Pacific shrimp Litopenaeus vannamei (Decapoda: Penaeidae) Ma. del Carmen Flores-Miranda Antonio Luna-González Ángel I. Campa Córdova Jesús A. Fierro-Coronado Blanca O. Partida-Arangure José Pintado Héctor A. González-Ocampo Biología vibriosis Vibrio sinaloensis enzymatic activity hemolytic activity Litopenaeus vannamei Infectious diseases especially those caused by bacterial and viral pathogens are serious loss factors in shrimp farming. In this study, bacteria were isolated from the gut and hepatopancreas of stressed shrimps obtained from a commercial farm. The isolates were screened on Thiosulfate citrate bile salt sucrose (TCBS) agar plates for the selection of Vibrio species. Presumptive vibrios were characterized through tests for hemolytic and enzymatic activity, hydrophobicity, growth and molecular identification. Three experimental infections were conducted in order to confirm the pathogenicity of selected bacterial strains VHPC18, VHPC23, VHPC24 and VIC30. In the third experimental challenge the LD50 was obtained, it lasted 10 days with 10 shrimp, weighing 6.9±1.1g, per tank. The treatments in triplicate were: (1) saline solution (control group); (2) 2×105CFU/shrimp; (3) 4×105CFU/shrimp; (4) 2×106CFU/shrimp; (5) 4×106CFU/shrimp, and (6) 8×106CFU/shrimp. In all challenges, water parameters measured during the experimental period remained within optimum ranges. Pathogenicity tests confirmed that the mixture of four vibrio isolates, identified as Vibrio sinaloensis, was virulent for L. vannamei. The LD50 value was 1.178×105CFU/g body weight. V. sinaloensis may act as opportunistic pathogens for cultured L. vannamei. 2012 artículo científico 0034-7744 https://www.redalyc.org/articulo.oa?id=44923872005 en http://www.redalyc.org/revista.oa?id=449 Revista de Biología Tropical application/pdf Universidad de Costa Rica Revista de Biología Tropical (Costa Rica) Num.2 Vol.60 |
| title | Isolation and characterization of infectious Vibrio sinaloensis strains from the Pacific shrimp Litopenaeus vannamei (Decapoda: Penaeidae) |
| topic | Biología vibriosis Vibrio sinaloensis enzymatic activity hemolytic activity Litopenaeus vannamei |
| url | https://www.redalyc.org/articulo.oa?id=44923872005 |