Mycorrhizal fungi isolated from native terrestrial orchids of pristine regions in Córdoba (Argentina)
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Universidad de Costa Rica
2015
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| _version_ | 1876484752375021568 |
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| author | Agustina Fernández Di Pardo |
| author_facet | Agustina Fernández Di Pardo |
| contents | Mycorrhizal fungi isolated from native terrestrial orchids of pristine regions in Córdoba (Argentina) Agustina Fernández Di Pardo Viviana M. Chiocchio Viviana Barrera Roxana P. Colombo Alicia E. Martinez Laura Gasoni Alicia M. Godeas Biología ITS rDNA Epulorhiza mycorrhiza Rhizoctonia Orchidaceae is a highly dependent group on the Rhizoctonia complex that includes Ceratorhiza, Moniliopsis, Epulorhiza and Rhizoctonia , for seed germination and the development of new orchid plants. Thus, the isolation and identification of orchid mycorrhizal fungi are important to understand the orchid-fungus rela - tionship, which can lead to the development of efficient conservation strategies by in vivo germination of seeds from endangered orchid plants. The aim of our work was to isolate and characterize the different mycorrhizal fungi found in roots of terrestrial orchids from Córdoba (Argentina), and, to learn about the natural habit and fungal associations in the Chaco Serrano woodland pristine region. In this study, bloomed orchid root and rhizo - sphere soil samples were obtained in two times from Valle de Punilla during spring of 2007; samples were kept in plastic bags until processed within 48 hours, and mycorrhizal condition confirmed assessing peloton pres - ence. A total of 23 isolates of the orchideous mycorrhizal Rhizoctonia complex were obtained. The isolates were studied based on morphological characters and ITS-rDNA sequences. Morphological characteristics as color of colonies, texture, growth rate, hyphal diameter and length and presence of sclerotia were observed on culture media. To define the number of nuclei per cell, the isolates were grown in Petri dishes containing water-agar (WA) for three days at 25°C and stained with Safranine-O solution. The mycorrhizal fungi were grouped into binucleate (MSGib, 10 isolates) and multinucleate (MSGim, 13 isolates) based on morphological characteristics of the colonies. We obtained the ITS1-5.8s-ITS4 region that was amplified using primers ITS1 and ITS4. Based on DNA sequencing, isolates Q23 and Q29 were found to be related to species of Ceratobasidium . Isolates Q24 and Q4 were related to the binucleated anastomosis group AG-C of Rhizoctonia sp. The rest of the isolates grouped in the Ceratobasidium clade without grouping. From our knowledge this is the first report of the asso - ciation of the AG-C testers with terrestrial orchids. A high specificity was observed in the symbiotic relationship. As the mycorrhizal fungal isolates were obtained from native orchids, they could be incorporated in conservation programes of endangered orchids in Argentina. 2015 artículo científico 0034-7744 https://www.redalyc.org/articulo.oa?id=44933764022 en http://www.redalyc.org/revista.oa?id=449 Revista de Biología Tropical application/pdf Universidad de Costa Rica Revista de Biología Tropical (Costa Rica) Num.1 Vol.63 |
| format | Artículo científico |
| id | redalyc_44933764022 |
| institution | Redalyc |
| language | en |
| publishDate | 2015 |
| publisher | Universidad de Costa Rica |
| spellingShingle | Mycorrhizal fungi isolated from native terrestrial orchids of pristine regions in Córdoba (Argentina) Agustina Fernández Di Pardo Biología ITS rDNA Epulorhiza mycorrhiza Rhizoctonia Mycorrhizal fungi isolated from native terrestrial orchids of pristine regions in Córdoba (Argentina) Agustina Fernández Di Pardo Viviana M. Chiocchio Viviana Barrera Roxana P. Colombo Alicia E. Martinez Laura Gasoni Alicia M. Godeas Biología ITS rDNA Epulorhiza mycorrhiza Rhizoctonia Orchidaceae is a highly dependent group on the Rhizoctonia complex that includes Ceratorhiza, Moniliopsis, Epulorhiza and Rhizoctonia , for seed germination and the development of new orchid plants. Thus, the isolation and identification of orchid mycorrhizal fungi are important to understand the orchid-fungus rela - tionship, which can lead to the development of efficient conservation strategies by in vivo germination of seeds from endangered orchid plants. The aim of our work was to isolate and characterize the different mycorrhizal fungi found in roots of terrestrial orchids from Córdoba (Argentina), and, to learn about the natural habit and fungal associations in the Chaco Serrano woodland pristine region. In this study, bloomed orchid root and rhizo - sphere soil samples were obtained in two times from Valle de Punilla during spring of 2007; samples were kept in plastic bags until processed within 48 hours, and mycorrhizal condition confirmed assessing peloton pres - ence. A total of 23 isolates of the orchideous mycorrhizal Rhizoctonia complex were obtained. The isolates were studied based on morphological characters and ITS-rDNA sequences. Morphological characteristics as color of colonies, texture, growth rate, hyphal diameter and length and presence of sclerotia were observed on culture media. To define the number of nuclei per cell, the isolates were grown in Petri dishes containing water-agar (WA) for three days at 25°C and stained with Safranine-O solution. The mycorrhizal fungi were grouped into binucleate (MSGib, 10 isolates) and multinucleate (MSGim, 13 isolates) based on morphological characteristics of the colonies. We obtained the ITS1-5.8s-ITS4 region that was amplified using primers ITS1 and ITS4. Based on DNA sequencing, isolates Q23 and Q29 were found to be related to species of Ceratobasidium . Isolates Q24 and Q4 were related to the binucleated anastomosis group AG-C of Rhizoctonia sp. The rest of the isolates grouped in the Ceratobasidium clade without grouping. From our knowledge this is the first report of the asso - ciation of the AG-C testers with terrestrial orchids. A high specificity was observed in the symbiotic relationship. As the mycorrhizal fungal isolates were obtained from native orchids, they could be incorporated in conservation programes of endangered orchids in Argentina. 2015 artículo científico 0034-7744 https://www.redalyc.org/articulo.oa?id=44933764022 en http://www.redalyc.org/revista.oa?id=449 Revista de Biología Tropical application/pdf Universidad de Costa Rica Revista de Biología Tropical (Costa Rica) Num.1 Vol.63 |
| title | Mycorrhizal fungi isolated from native terrestrial orchids of pristine regions in Córdoba (Argentina) |
| topic | Biología ITS rDNA Epulorhiza mycorrhiza Rhizoctonia |
| url | https://www.redalyc.org/articulo.oa?id=44933764022 |