Histochemical differences along the intestine of Corydoras paleatus (Siluriformes: Callichthyidae)
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Universidad de Costa Rica
2016
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| _version_ | 1876460699081768960 |
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| author | Silvia E. Plaul |
| author_facet | Silvia E. Plaul |
| contents | Histochemical differences along the intestine of Corydoras paleatus (Siluriformes: Callichthyidae) Silvia E. Plaul Claudio G. Barbeito Alcira O. Díaz Biología intestine goblet cells histochemistry glycoconjugates Corydoras paleatus The Neotropical catfish Corydoras paleatus is a facultative air-breather and the caudal half of theintestine is involved in gas exchange. In South America, air-breathing fishes are found in tropical or sub-tropicalfreshwaters where the probability of hypoxia is high. The aim of this study was to characterize by traditionalhistochemical and lectinhistochemical methods the pattern of carbohydrate in the intestinal mucosa. Intestinesamples were taken from 25 healthy adult specimens collected in Buenos Aires (Argentina). Samples were fixedby immersion in 10 % buffered formalin and routinely processed and embedded in paraffin wax. Subsequently,these sections were incubated in the biotinylated lectins battery. Labeled Streptavidin-Biotin (LSAB) system wasused for detection, diaminobenzidine as chromogen and haematoxylin as a contrast. To locate and distinguishglycoconjugates (GCs) of the globet cells, we used the following histochemical methods: PAS; PAS*S; KOH/PA*S; PA/Bh/KOH/PAS; KOH/PA*/Bh/PAS; Alcian Blue and Toluidine Blue at different pHs. Microscopically,the general structure of vertebrate intestine was observed and showed all the cell types characteristic of theintestinal epithelium. The cranial sector of catfish intestine is a site of digestion and absorption and its structureis similar to other fish groups. In contrast, enterocytes of the caudal portion are low cuboidal cells; and betweenthese, globet cells and capillaries are observed, these latter may reach the mucosal lumen. Underlying the epithelium,observed a well-developed lamina propria-submucosa made of connective tissue; this layer was highlyvascularized and did not exhibit glands. According to histochemistry, the diverse GCs elaborated and secretedin the intestine are associated with specific functions in relation to their physiological significance, with specialreference to their role in lubrication, buffering effect and prevention of proteolytic damage to the epitheliumtogether with other biological processes, such as osmoregulation and ion exchange. The lectinhistochemicalanalysis of the intestinal mucosa reveals the presence of terminal residues of glucose, mannose and galactose.In conclusion, this study has shown that GCs synthesized in the intestine of C. paleatus exhibit a high level ofhistochemical complexity and that the lectin binding pattern of the intestinal mucosa is characteristic of eachspecies and the variations are related with the multiple functions performed by the mucus in the digestive tract.The information generated here may be a relevant biological tool for comparing and analyzing the possibleglycosidic changes in the intestinal mucus under different conditions, such as changes in diet or different pathologicalstages. 2016 artículo científico 0034-7744 https://www.redalyc.org/articulo.oa?id=44943437028 en http://www.redalyc.org/revista.oa?id=449 Revista de Biología Tropical application/pdf Universidad de Costa Rica Revista de Biología Tropical (Costa Rica) Num.1 Vol.64 |
| format | Artículo científico |
| id | redalyc_44943437028 |
| institution | Redalyc |
| language | en |
| publishDate | 2016 |
| publisher | Universidad de Costa Rica |
| spellingShingle | Histochemical differences along the intestine of Corydoras paleatus (Siluriformes: Callichthyidae) Silvia E. Plaul Biología intestine goblet cells histochemistry glycoconjugates Corydoras paleatus Histochemical differences along the intestine of Corydoras paleatus (Siluriformes: Callichthyidae) Silvia E. Plaul Claudio G. Barbeito Alcira O. Díaz Biología intestine goblet cells histochemistry glycoconjugates Corydoras paleatus The Neotropical catfish Corydoras paleatus is a facultative air-breather and the caudal half of theintestine is involved in gas exchange. In South America, air-breathing fishes are found in tropical or sub-tropicalfreshwaters where the probability of hypoxia is high. The aim of this study was to characterize by traditionalhistochemical and lectinhistochemical methods the pattern of carbohydrate in the intestinal mucosa. Intestinesamples were taken from 25 healthy adult specimens collected in Buenos Aires (Argentina). Samples were fixedby immersion in 10 % buffered formalin and routinely processed and embedded in paraffin wax. Subsequently,these sections were incubated in the biotinylated lectins battery. Labeled Streptavidin-Biotin (LSAB) system wasused for detection, diaminobenzidine as chromogen and haematoxylin as a contrast. To locate and distinguishglycoconjugates (GCs) of the globet cells, we used the following histochemical methods: PAS; PAS*S; KOH/PA*S; PA/Bh/KOH/PAS; KOH/PA*/Bh/PAS; Alcian Blue and Toluidine Blue at different pHs. Microscopically,the general structure of vertebrate intestine was observed and showed all the cell types characteristic of theintestinal epithelium. The cranial sector of catfish intestine is a site of digestion and absorption and its structureis similar to other fish groups. In contrast, enterocytes of the caudal portion are low cuboidal cells; and betweenthese, globet cells and capillaries are observed, these latter may reach the mucosal lumen. Underlying the epithelium,observed a well-developed lamina propria-submucosa made of connective tissue; this layer was highlyvascularized and did not exhibit glands. According to histochemistry, the diverse GCs elaborated and secretedin the intestine are associated with specific functions in relation to their physiological significance, with specialreference to their role in lubrication, buffering effect and prevention of proteolytic damage to the epitheliumtogether with other biological processes, such as osmoregulation and ion exchange. The lectinhistochemicalanalysis of the intestinal mucosa reveals the presence of terminal residues of glucose, mannose and galactose.In conclusion, this study has shown that GCs synthesized in the intestine of C. paleatus exhibit a high level ofhistochemical complexity and that the lectin binding pattern of the intestinal mucosa is characteristic of eachspecies and the variations are related with the multiple functions performed by the mucus in the digestive tract.The information generated here may be a relevant biological tool for comparing and analyzing the possibleglycosidic changes in the intestinal mucus under different conditions, such as changes in diet or different pathologicalstages. 2016 artículo científico 0034-7744 https://www.redalyc.org/articulo.oa?id=44943437028 en http://www.redalyc.org/revista.oa?id=449 Revista de Biología Tropical application/pdf Universidad de Costa Rica Revista de Biología Tropical (Costa Rica) Num.1 Vol.64 |
| title | Histochemical differences along the intestine of Corydoras paleatus (Siluriformes: Callichthyidae) |
| topic | Biología intestine goblet cells histochemistry glycoconjugates Corydoras paleatus |
| url | https://www.redalyc.org/articulo.oa?id=44943437028 |