Endophytic fungi from tropical ethnoveterinary plants and their antibacterial efficacy against Pasteurella multocida Capsular Type A strain
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Universidad de Costa Rica
2016
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| _version_ | 1876445259109498880 |
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| author | Archana Nath |
| author_facet | Archana Nath |
| contents | Endophytic fungi from tropical ethnoveterinary plants and their antibacterial efficacy against Pasteurella multocida Capsular Type A strain Archana Nath Santa Ram Joshi Biología antibacterial endophytic fungi Pasteurella multocida Ethnoveterinary plants scanning electron microscopy Pasteurella multocida is an important veterinary pathogen causing infections in animals and birds. Nowadays, different reports have described the severity of infections, increasing resistance of micro-organisms to antibiotics, and the contribution of ethnoveterinary practices towards the treatment of various ailments of animals. The aim of the present study was to investigate the antibacterial efficacy of the ethanolic extracts of endophytic fungi against P. multocida Capsular Type A strains. A total of six endophytic fungi were isolated from two tropical ethnoveterinary plants: Garcinia xanthochymus H. and Polygonum chinense L. The ethanolic extracts of the endophytic fungi were subjected to in vitro antimicrobial activity by the well diffusion method. Besides, we evaluated the treatment of mice with the potent fungal extract and observed the effects in different organs under electron microscopy. Our results showed that four fungi had antimicrobial activity against the selected pathogen. The best antibacterial activity was showed by the extract of the endophytic fungi, Glomerella magna isolated from G. xanthochymus, with a minimum inhibitory concentration of 46.9 μg/mL and minimum bactericidal concentration of 750 μg/mL. Treatment of mice with the potent fungal extract caused a considerable inhibitory effect on the pathogen growth in vital organs, results that was also confirmed by histopathological studies made by scanning electron microscopy. The present findings indicated that the endophytic fungi G. magna has the potential to provide an effective treatment against infections caused by Pasteurella multocida. However, the isolation of bioactive components needs further investigation. 2016 artículo científico 0034-7744 https://www.redalyc.org/articulo.oa?id=44945722022 https://www.redalyc.org/journal/449/44945722022/ https://www.redalyc.org/journal/449/44945722022/html/ https://www.redalyc.org/journal/449/44945722022/44945722022.epub https://www.redalyc.org/journal/449/44945722022/movil en http://www.redalyc.org/revista.oa?id=449 Revista de Biología Tropical application/pdf Universidad de Costa Rica Revista de Biología Tropical (Costa Rica) Num.2 Vol.64 |
| format | Artículo científico |
| id | redalyc_44945722022 |
| institution | Redalyc |
| language | en |
| publishDate | 2016 |
| publisher | Universidad de Costa Rica |
| spellingShingle | Endophytic fungi from tropical ethnoveterinary plants and their antibacterial efficacy against Pasteurella multocida Capsular Type A strain Archana Nath Biología antibacterial endophytic fungi Pasteurella multocida Ethnoveterinary plants scanning electron microscopy Endophytic fungi from tropical ethnoveterinary plants and their antibacterial efficacy against Pasteurella multocida Capsular Type A strain Archana Nath Santa Ram Joshi Biología antibacterial endophytic fungi Pasteurella multocida Ethnoveterinary plants scanning electron microscopy Pasteurella multocida is an important veterinary pathogen causing infections in animals and birds. Nowadays, different reports have described the severity of infections, increasing resistance of micro-organisms to antibiotics, and the contribution of ethnoveterinary practices towards the treatment of various ailments of animals. The aim of the present study was to investigate the antibacterial efficacy of the ethanolic extracts of endophytic fungi against P. multocida Capsular Type A strains. A total of six endophytic fungi were isolated from two tropical ethnoveterinary plants: Garcinia xanthochymus H. and Polygonum chinense L. The ethanolic extracts of the endophytic fungi were subjected to in vitro antimicrobial activity by the well diffusion method. Besides, we evaluated the treatment of mice with the potent fungal extract and observed the effects in different organs under electron microscopy. Our results showed that four fungi had antimicrobial activity against the selected pathogen. The best antibacterial activity was showed by the extract of the endophytic fungi, Glomerella magna isolated from G. xanthochymus, with a minimum inhibitory concentration of 46.9 μg/mL and minimum bactericidal concentration of 750 μg/mL. Treatment of mice with the potent fungal extract caused a considerable inhibitory effect on the pathogen growth in vital organs, results that was also confirmed by histopathological studies made by scanning electron microscopy. The present findings indicated that the endophytic fungi G. magna has the potential to provide an effective treatment against infections caused by Pasteurella multocida. However, the isolation of bioactive components needs further investigation. 2016 artículo científico 0034-7744 https://www.redalyc.org/articulo.oa?id=44945722022 https://www.redalyc.org/journal/449/44945722022/ https://www.redalyc.org/journal/449/44945722022/html/ https://www.redalyc.org/journal/449/44945722022/44945722022.epub https://www.redalyc.org/journal/449/44945722022/movil en http://www.redalyc.org/revista.oa?id=449 Revista de Biología Tropical application/pdf Universidad de Costa Rica Revista de Biología Tropical (Costa Rica) Num.2 Vol.64 |
| title | Endophytic fungi from tropical ethnoveterinary plants and their antibacterial efficacy against Pasteurella multocida Capsular Type A strain |
| topic | Biología antibacterial endophytic fungi Pasteurella multocida Ethnoveterinary plants scanning electron microscopy |
| url | https://www.redalyc.org/articulo.oa?id=44945722022 https://www.redalyc.org/journal/449/44945722022/ https://www.redalyc.org/journal/449/44945722022/html/ https://www.redalyc.org/journal/449/44945722022/44945722022.epub https://www.redalyc.org/journal/449/44945722022/movil |