In vitro antioxidant activity and qualitative phytochemical analysis of two Vismia (Hypericaceae) species collected in Los Andes, Venezuela
Fuente:
Redalyc
Salvato in:
| Autore principale: | |
|---|---|
| Natura: | Artículo científico |
| Lingua: | en |
| Pubblicazione: |
Universidad de Costa Rica
2016
|
| Soggetti: | |
| Accesso online: | |
| Tags: |
Aggiungi Tag
Nessun Tag, puoi essere il primo ad aggiungerne!!
|
| _version_ | 1876471265751990272 |
|---|---|
| author | Alexis Buitrago D. |
| author_facet | Alexis Buitrago D. |
| contents | In vitro antioxidant activity and qualitative phytochemical analysis of two Vismia (Hypericaceae) species collected in Los Andes, Venezuela Alexis Buitrago D. Janne Rojas-Vera Yonel Peñaloza Biología DPPH cal analysis Vismia baccifera Vismia macrophylla Antioxidant activity Vismia genus is distributed mainly in tropical and subtropical regions of Central, South America and some areas of Africa. According to previous investigations, antioxidant potential of Vismia species might be related to anthrones, anthraquinones, flavonoids and phenol derivatives biosynthesized by these plants. In this investigation, phytochemical screening of Vismia baccifera (VB) from Mérida-Venezuela and Vismia macrophylla (VM) from Táchira-Venezuela methanolic extracts, carried out using various chemical assays, revealed an abundant presence of anthraquinones in both species analyzed. Glycosides were also present while flavones and dehydroflavones were observed abundantly in VB but moderated in VM. Triterpenes were also detected and steroids showed to be abundant in VM but moderate in VB. On the other hand, antioxidant capacity measured by the DPPH assay showed that VM possesses a stronger antioxidant activity than VB with IC50 5.50 μg mL-1. Phenol and flavonoid assays carried out by Folin-Ciocalteu and colorimetric test also revealed that methanol extracts of both species contain high concentrations of these metabolites. A relationship between the antioxidant activity, total phenol and flavonoids content of the extracts analyzed was demonstrated in this investigation since those samples with higher phenolic concentrations showed likewise higher antioxidant activity. Rev. Biol. Trop. 64 (4): 1431-1439. Epub 2016 December 01. 2016 artículo científico 0034-7744 https://www.redalyc.org/articulo.oa?id=44947539006 https://www.redalyc.org/journal/449/44947539006/ https://www.redalyc.org/journal/449/44947539006/html/ https://www.redalyc.org/journal/449/44947539006/44947539006.epub https://www.redalyc.org/journal/449/44947539006/movil en http://www.redalyc.org/revista.oa?id=449 Revista de Biología Tropical application/pdf Universidad de Costa Rica Revista de Biología Tropical (Costa Rica) Num.4 Vol.64 |
| format | Artículo científico |
| id | redalyc_44947539006 |
| institution | Redalyc |
| language | en |
| publishDate | 2016 |
| publisher | Universidad de Costa Rica |
| spellingShingle | In vitro antioxidant activity and qualitative phytochemical analysis of two Vismia (Hypericaceae) species collected in Los Andes, Venezuela Alexis Buitrago D. Biología DPPH cal analysis Vismia baccifera Vismia macrophylla Antioxidant activity In vitro antioxidant activity and qualitative phytochemical analysis of two Vismia (Hypericaceae) species collected in Los Andes, Venezuela Alexis Buitrago D. Janne Rojas-Vera Yonel Peñaloza Biología DPPH cal analysis Vismia baccifera Vismia macrophylla Antioxidant activity Vismia genus is distributed mainly in tropical and subtropical regions of Central, South America and some areas of Africa. According to previous investigations, antioxidant potential of Vismia species might be related to anthrones, anthraquinones, flavonoids and phenol derivatives biosynthesized by these plants. In this investigation, phytochemical screening of Vismia baccifera (VB) from Mérida-Venezuela and Vismia macrophylla (VM) from Táchira-Venezuela methanolic extracts, carried out using various chemical assays, revealed an abundant presence of anthraquinones in both species analyzed. Glycosides were also present while flavones and dehydroflavones were observed abundantly in VB but moderated in VM. Triterpenes were also detected and steroids showed to be abundant in VM but moderate in VB. On the other hand, antioxidant capacity measured by the DPPH assay showed that VM possesses a stronger antioxidant activity than VB with IC50 5.50 μg mL-1. Phenol and flavonoid assays carried out by Folin-Ciocalteu and colorimetric test also revealed that methanol extracts of both species contain high concentrations of these metabolites. A relationship between the antioxidant activity, total phenol and flavonoids content of the extracts analyzed was demonstrated in this investigation since those samples with higher phenolic concentrations showed likewise higher antioxidant activity. Rev. Biol. Trop. 64 (4): 1431-1439. Epub 2016 December 01. 2016 artículo científico 0034-7744 https://www.redalyc.org/articulo.oa?id=44947539006 https://www.redalyc.org/journal/449/44947539006/ https://www.redalyc.org/journal/449/44947539006/html/ https://www.redalyc.org/journal/449/44947539006/44947539006.epub https://www.redalyc.org/journal/449/44947539006/movil en http://www.redalyc.org/revista.oa?id=449 Revista de Biología Tropical application/pdf Universidad de Costa Rica Revista de Biología Tropical (Costa Rica) Num.4 Vol.64 |
| title | In vitro antioxidant activity and qualitative phytochemical analysis of two Vismia (Hypericaceae) species collected in Los Andes, Venezuela |
| topic | Biología DPPH cal analysis Vismia baccifera Vismia macrophylla Antioxidant activity |
| url | https://www.redalyc.org/articulo.oa?id=44947539006 https://www.redalyc.org/journal/449/44947539006/ https://www.redalyc.org/journal/449/44947539006/html/ https://www.redalyc.org/journal/449/44947539006/44947539006.epub https://www.redalyc.org/journal/449/44947539006/movil |