Cinética de crecimiento in vitro de dos cepas de Trypanosoma cruzi (Kinetoplastida: Trypanosomatidae) en células miocárdicas de roedores con diferente susceptibilidad
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| Natura: | Artículo científico |
| Lingua: | en |
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Universidad de Costa Rica
2007
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| _version_ | 1876443853536362496 |
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| author | Rafael-Oswaldo Angel B. |
| author_facet | Rafael-Oswaldo Angel B. |
| contents | Cinética de crecimiento in vitro de dos cepas de Trypanosoma cruzi (Kinetoplastida: Trypanosomatidae) en células miocárdicas de roedores con diferente susceptibilidad Rafael-Oswaldo Angel B. Misael Chinchilla C. Olga Marta Guerrero B. Alfredo Castro C. Biología heart cells cell culture Trypanosomatidae Trypanosoma cruzi In vitro growth kinetics of two Trypanosoma cruzi (Kinetoplastida: Trypanosomatidae) clonesin myocardial cells from rodents of different susceptibility. Two Trypanosoma cruzi isolates, TCR-4 fromCosta Rica and UES-1 from El Salvador, were studied in vitro to compare their infectivity or resistance andintracellular replication in myocardial cells in three strains of mice and rats: NGP white mice, C3 H mice andSprague Dowley rats. Myocardial cells were cultured on coverslips at 37 º C in a humid 10 % CO2 atmosphereand then infected at a ratio of one tripomastigote per cell. Samples were studied after 24, 72, 96 and 120 h ofinfection to determine parasite infection capacity and intracellular multiplication. Both parasites had the highestinfection capacity in C3 H mice, followed by NGP mice cells with a very low infection rate. Lastly, almost noTrypanosoma cruzi multiplication was observed in Sprague Dowley rats, suggesting a strong natural resistancein this animal to both strains of the parasite. The UES-1 isolate presented higher multiplication and greater invasionthan the TCR-4 strain, showing greater virulence of UES-1 in heart cells, at least in vitro. Rev. Biol. Trop.55 (1): 121-126. Epub 2007 March. 31. 2007 artículo científico 0034-7744 https://www.redalyc.org/articulo.oa?id=44955112 en http://www.redalyc.org/revista.oa?id=449 Revista de Biología Tropical application/pdf Universidad de Costa Rica Revista de Biología Tropical (Costa Rica) Num.1 Vol.55 |
| format | Artículo científico |
| id | redalyc_44955112 |
| institution | Redalyc |
| language | en |
| publishDate | 2007 |
| publisher | Universidad de Costa Rica |
| spellingShingle | Cinética de crecimiento in vitro de dos cepas de Trypanosoma cruzi (Kinetoplastida: Trypanosomatidae) en células miocárdicas de roedores con diferente susceptibilidad Rafael-Oswaldo Angel B. Biología heart cells cell culture Trypanosomatidae Trypanosoma cruzi Cinética de crecimiento in vitro de dos cepas de Trypanosoma cruzi (Kinetoplastida: Trypanosomatidae) en células miocárdicas de roedores con diferente susceptibilidad Rafael-Oswaldo Angel B. Misael Chinchilla C. Olga Marta Guerrero B. Alfredo Castro C. Biología heart cells cell culture Trypanosomatidae Trypanosoma cruzi In vitro growth kinetics of two Trypanosoma cruzi (Kinetoplastida: Trypanosomatidae) clonesin myocardial cells from rodents of different susceptibility. Two Trypanosoma cruzi isolates, TCR-4 fromCosta Rica and UES-1 from El Salvador, were studied in vitro to compare their infectivity or resistance andintracellular replication in myocardial cells in three strains of mice and rats: NGP white mice, C3 H mice andSprague Dowley rats. Myocardial cells were cultured on coverslips at 37 º C in a humid 10 % CO2 atmosphereand then infected at a ratio of one tripomastigote per cell. Samples were studied after 24, 72, 96 and 120 h ofinfection to determine parasite infection capacity and intracellular multiplication. Both parasites had the highestinfection capacity in C3 H mice, followed by NGP mice cells with a very low infection rate. Lastly, almost noTrypanosoma cruzi multiplication was observed in Sprague Dowley rats, suggesting a strong natural resistancein this animal to both strains of the parasite. The UES-1 isolate presented higher multiplication and greater invasionthan the TCR-4 strain, showing greater virulence of UES-1 in heart cells, at least in vitro. Rev. Biol. Trop.55 (1): 121-126. Epub 2007 March. 31. 2007 artículo científico 0034-7744 https://www.redalyc.org/articulo.oa?id=44955112 en http://www.redalyc.org/revista.oa?id=449 Revista de Biología Tropical application/pdf Universidad de Costa Rica Revista de Biología Tropical (Costa Rica) Num.1 Vol.55 |
| title | Cinética de crecimiento in vitro de dos cepas de Trypanosoma cruzi (Kinetoplastida: Trypanosomatidae) en células miocárdicas de roedores con diferente susceptibilidad |
| topic | Biología heart cells cell culture Trypanosomatidae Trypanosoma cruzi |
| url | https://www.redalyc.org/articulo.oa?id=44955112 |