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| Format: | Artículo científico |
| Language: | en |
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Universidad de Costa Rica
2007
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| Online Access: | https://www.redalyc.org/articulo.oa?id=44955206 |
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Table of Contents:
- Actividades enzimáticas en aislamientos bacterianos de tractos digestivos de larvas y del contenido de pupas de Automeris zugana y Rothschildia lebeau (Lepidoptera: Saturniidae) Adrián Pinto-Tomás Lorena Uribe-Lorío John Blanco Gustavo Fontecha César Rodríguez Marielos Mora Daniel Janzen Felipe Chavarría Joel Díaz Ana Sittenfeld Biología Costa Rica Saturniidae caterpillar Automeris zugana bacterial enzymes Enzymatic activities of bacteria isolated from the digestive tract of caterpillars and the pupalcontent of Automeris zugana and Rothschildia lebeau (Lepidoptera: Saturniidae). The enzymatic activitiesof bacteria isolated from the digestive tracts of caterpillars and the pupal contents of Automeris zugana andRothschildia lebeau was studied. This digestive tract represents an extreme microenvironment due to its highpH and presence of antimicrobial substances secreted by the insect or derived from ingested plant tissue. At thesame time, it contains large amounts of nutrient-rich food, for which microbes may compete among themselvesand with the caterpillar. There is little information about the microbiota associated with tropical caterpillar guts,although bacteria from different genera have been isolated from gut and pupae samples. The study of the enzymaticactivities generated by these organisms constitutes a starting point to understand their metabolic and physiologicalrelationships with their hosts, and to find enzymes that have potential biotechnological applications. Inthis study we evaluated several enzymatic activities in two collections of bacteria isolated from caterpillar gutsand pupae of the tropical lepidopteran species A. zugana and R. lebeau. Bacteria grown under aerobic conditionswere tested for an array of enzymes, including gelatinases, caseinases, lipases, esterases, cellulases, xylanases,amylases and chitinases. Both collections displayed similar patterns of enzymatic activity. No isolate showedactivity for all enzymatic tests, but as a whole, at least some bacteria in each collection were able to degradeeach substrate tested. Isolates with the same taxonomic identification obtained from caterpillar guts and pupaehad almost the same enzymatic activities. In both collections, it was possible to group bacterial isolates accordingto their enzyme activity pattern. In addition to a heterogeneous ensemble of isolates exhibiting two or lessenzymatic activities, there were two groups with at least five activities that showed an apparent specialization forthe substrates they were able to use. The first consisted exclusively of isolates of the family Enterobacteriaceae,which were positive for lipolytic and chitinolytic activities, but completely lacked amylasic, cellulolytic and xylanolyticactivities. The second group, composed mainly of Gram-positive rods, exhibited the opposite pattern:they were positive for amylasic, cellulolytic and xylanolytic activities, lacked chitinolytic activity and had fewisolates with lipolytic activity. This work forms the foundation for future research to explore the biotechnologicalpotential of bacterial isolates from caterpillar guts. Rev. Biol. Trop. 55 (2): 401-415. Epub 2007 June, 29. 2007 artículo científico 0034-7744 https://www.redalyc.org/articulo.oa?id=44955206 en http://www.redalyc.org/revista.oa?id=449 Revista de Biología Tropical application/pdf Universidad de Costa Rica Revista de Biología Tropical (Costa Rica) Num.2 Vol.55