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| Format: | Artículo científico |
| Language: | en |
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Universidad de Costa Rica
2007
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| Online Access: | https://www.redalyc.org/articulo.oa?id=44955209 |
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Table of Contents:
- Molecular phylogenetic relationships among four species of the mangrove tree genus Bruguiera (Rhizophoraceae), as revealed by chromosome and RAPD markers Pragnya Sahoo Satyanarayan Jena Suprava Mohanty Anath Bandhu Das Biología Bruguiera karyotype tree mangrove 4C DNA content molecular phylogeny Analysis of karyotype, nuclear DNA content and RAPD markers were performed in four speciesof Bruguiera (Rhizophoraceae) of Bhitarkanika mangrove forests, Orissa, India. Detailed karyotype analysisrevealing 2n=34 in B. cylindrica and 2n=36 in B. gymnorrhiza was reported for the first time and 2n=34 in B.parviflora and B. sexangula was confirmed. On the basis of the common types of chromosomes present amongBruguiera, two distinct groups were found; one consists of B. cylindrica and B. parviflora and the other of B.gymnorrhiza and B. sexangula. The symmetrical karyotype with same chromosome types grouped B. cylindricaand B. parviflora together and presence of Type E chromosomes placed B. gymnorrhiza and B. sexangula in aseparate group, suggesting their closer affinity in their respective group. Analysis of chromosome length, volume,INV and 4C DNA content confirmed this division. Nuclear DNA content was two-fold higher (~17.0 pg)in the second group than in the first (~8.0 pg). The amplification products generated through RAPD revealed 1-9amplicons with size variations from 600 bp to 2 500 bp with 49.31% genetic similarity between B. gymnorrhizaand B. sexangula and 47.10% in between B. cylindrica and B. parviflora. The high copy number marker band(~ 1 100 bp) yielded in OPN-15 primer in B. parviflora the characteristic DNA marker, which was cloned andused as probes for assessment of genetic diversity, and demonstrated its close genetic affinity to B. cylindrica.B. gymnorrhiza and B. sexangula also produced similar marker bands of ~600 bp and ~2 200 bp in the sameprimer. All of the cytological, 4C DNA content and RAPD data confirmed the existence of two taxonomicallydistinct groups of Bruguiera: one consisting of B. cylindrica and B. parviflora and the other of B. gymnorrhizaand B. sexangula as placed earlier (1862) in the tribe Rhizophoreae by Bentham and Hooker, on the basis of theflowering habits of Bruguiera. Genetically, the B. sexangula and B. gymnorrhiza group was found to be veryclosely, rather than distantly, related to B. parviflora and B. cylindrica. Our results demonstrate that molecularmarkers together with cytological evidence provide an effective tool to access the existing interspecific geneticpolymorphism in mangrove species, to solve the taxonomic problems and to design their conservation strategy.Rev. Biol. Trop. 55 (2): 437-448. Epub 2007 June, 29. 2007 artículo científico 0034-7744 https://www.redalyc.org/articulo.oa?id=44955209 en http://www.redalyc.org/revista.oa?id=449 Revista de Biología Tropical application/pdf Universidad de Costa Rica Revista de Biología Tropical (Costa Rica) Num.2 Vol.55