Hardening of the national flower of Colombia, the threatened Cattleya trianae (Orchidaceae), from in vitro culture with previous invigoration phase

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Main Author: Marcela Franco
Format: Artículo científico
Language:en
Published: Universidad de Costa Rica 2007
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author Marcela Franco
author_facet Marcela Franco
contents Hardening of the national flower of Colombia, the threatened Cattleya trianae (Orchidaceae), from in vitro culture with previous invigoration phase Marcela Franco Giovany Guevara Neftali Mesa Gloria Urueña Biología orchids hardening substrates ornamental acclimation Cattleya trianae is an endemic species from the tropical rainforest in the Colombian Andes. Itssurvival is currently threatened due to habitat loss and commercial overexploitation. This study evaluates tensubstrates, some organic (pine bark, coconut fiber and wood shavings), some inert icopor (polystyrene foam),vegetable coal and their combinations, and the effects these have on morphometric and phenotypic traits in thehardening phase of 250 plants of C. trianae cultivated in vitro. Recorded data include percent survival, length oflongest leaf, biomass (wet weight) and number of roots and leaves at the beginning and at the end of the experiment.After the hardening phase, the plants were taken to a greenhouse and later to the natural environment.Coconut fiber alone or mixed in equal parts with pine bark and coal was the most efficient substrate when percentsurvival (80±SE=0.3742), biomass, and leaf length were evaluated. Hardened plants displayed qualitativecharacteristics such as vigor, hardness and waxy texture, strength of green coloration in the leaves, and velamenformation. Under greenhouse conditions, plants grew better with filtered light, relative humidity bordering on80 %, permanent aeration, misting with water, and an average temperature of 25±2 °C. Invigorated plants werefirmly anchored on their host trees. Rev. Biol. Trop. 55 (2): 681-691. Epub 2007 June, 29. 2007 artículo científico 0034-7744 https://www.redalyc.org/articulo.oa?id=44955231 en http://www.redalyc.org/revista.oa?id=449 Revista de Biología Tropical application/pdf Universidad de Costa Rica Revista de Biología Tropical (Costa Rica) Num.2 Vol.55
format Artículo científico
id redalyc_44955231
institution Redalyc
language en
publishDate 2007
publisher Universidad de Costa Rica
spellingShingle Hardening of the national flower of Colombia, the threatened Cattleya trianae (Orchidaceae), from in vitro culture with previous invigoration phase
Marcela Franco
Biología
orchids
hardening
substrates
ornamental
acclimation
Hardening of the national flower of Colombia, the threatened Cattleya trianae (Orchidaceae), from in vitro culture with previous invigoration phase Marcela Franco Giovany Guevara Neftali Mesa Gloria Urueña Biología orchids hardening substrates ornamental acclimation Cattleya trianae is an endemic species from the tropical rainforest in the Colombian Andes. Itssurvival is currently threatened due to habitat loss and commercial overexploitation. This study evaluates tensubstrates, some organic (pine bark, coconut fiber and wood shavings), some inert icopor (polystyrene foam),vegetable coal and their combinations, and the effects these have on morphometric and phenotypic traits in thehardening phase of 250 plants of C. trianae cultivated in vitro. Recorded data include percent survival, length oflongest leaf, biomass (wet weight) and number of roots and leaves at the beginning and at the end of the experiment.After the hardening phase, the plants were taken to a greenhouse and later to the natural environment.Coconut fiber alone or mixed in equal parts with pine bark and coal was the most efficient substrate when percentsurvival (80±SE=0.3742), biomass, and leaf length were evaluated. Hardened plants displayed qualitativecharacteristics such as vigor, hardness and waxy texture, strength of green coloration in the leaves, and velamenformation. Under greenhouse conditions, plants grew better with filtered light, relative humidity bordering on80 %, permanent aeration, misting with water, and an average temperature of 25±2 °C. Invigorated plants werefirmly anchored on their host trees. Rev. Biol. Trop. 55 (2): 681-691. Epub 2007 June, 29. 2007 artículo científico 0034-7744 https://www.redalyc.org/articulo.oa?id=44955231 en http://www.redalyc.org/revista.oa?id=449 Revista de Biología Tropical application/pdf Universidad de Costa Rica Revista de Biología Tropical (Costa Rica) Num.2 Vol.55
title Hardening of the national flower of Colombia, the threatened Cattleya trianae (Orchidaceae), from in vitro culture with previous invigoration phase
topic Biología
orchids
hardening
substrates
ornamental
acclimation
url https://www.redalyc.org/articulo.oa?id=44955231