Saved in:
| Main Author: | |
|---|---|
| Format: | Artículo científico |
| Language: | es |
| Published: |
Universidad de Costa Rica
2019
|
| Subjects: | |
| Online Access: | https://www.redalyc.org/articulo.oa?id=44965908005 https://www.redalyc.org/journal/449/44965908005/ https://www.redalyc.org/journal/449/44965908005/html/ https://www.redalyc.org/journal/449/44965908005/44965908005.epub https://www.redalyc.org/journal/449/44965908005/movil |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1866811698361401344 |
|---|---|
| author | Laura Alejandra Mejía-Agudelo |
| author_facet | Laura Alejandra Mejía-Agudelo |
| contents | Morfoanatomía e histoquímica de Hypericum juniperinum (Hypericaceae) Laura Alejandra Mejía-Agudelo Juan Camilo Marín-Loaiza Xavier Marquínez-Casas Biología canals histochemistry phenolic compounds translucent glands Anatomical characterization Hypericum is a genus that belongs to the Hypericaceae family, with has 469 species of herbs, trees and shrubs. Two sections in particular, Brathys and Trygynobrathys are an integral part of the Paramo flora. Hypericum genus has a wide variety of metabolites that are associated with multiple pharmacological activities, and specifically in H. juniperinum antidepressant properties related to the presence of flavonoids and phenolic compounds have been reported. The objective of this research is to relate the anatomical structures with the metabolites detected by histochemical methods present in the vegetative and reproductive organs of Hypericum juniperinum collected in the Colombian Paramo. The aerial organs (stems, leaves and flowers) of Hypericum juniperinum were collected in the village Arbolocos of the municipality of Cuitiva, Boyacá (voucher code No. 589611, COL). For the anatomical analysis, the organs of H. juniperinum were processed with fixation techniques, incorporation in paraffin, cutting in microtome and staining with Astra-blue and basic fuchsin; for the histochemical analysis freehand cuts were made with specific stains for lipids, phenol compounds, alkaloids and pectins. The anatomy of the organs of H. juniperinum should be understood in the context of the living conditions of the Paramo. Leaves are highly xeromorphic as they are chartaceous, rosette-shaped, convolute and upright, with parenchyma in bistratified palisade, thickened walls, thick cuticle and epicuticular wax in both foliar epidermis, stomata on the abaxial side and with epidermal chamber. However, it has some mesomorphic characteristics such as parenchymatic bundle sheath and loose spongy parenchyma. Hypericum juniperinum has translucent glands in leaves, calyx and gynoecium, and lacks black glands typical of the Hypericum section belonging to the medicinal plant Hypericum perforatum. Histochemical analysis showed lipids in leaf canals, radial parenchyma of the stem and in some floral structures. Phenolic compounds were detected along the leaf, in the phloem of the stem and in the anthers. Hypericum juniperinum lacks alkaloids and presents pectins in the parenchyma of the sheath surrounding the vascular bundle of the leaf. The present work contributes by relating the metabolites with their distribution in the tissues of this native species. In addition, the anatomy of H. juniperinum has characteristics of the Paramo plants. Description given here could be used as a preliminary monograph of plants from the Hypericum genus with promising therapeutic properties. 2019 artículo científico 0034-7744 https://www.redalyc.org/articulo.oa?id=44965908005 https://www.redalyc.org/journal/449/44965908005/ https://www.redalyc.org/journal/449/44965908005/html/ https://www.redalyc.org/journal/449/44965908005/44965908005.epub https://www.redalyc.org/journal/449/44965908005/movil DOI 10.15517/RBT.V67I6.36072 es http://www.redalyc.org/revista.oa?id=449 Revista de Biología Tropical application/pdf Universidad de Costa Rica Revista de Biología Tropical (Costa Rica) Num.6 Vol.67 |
| format | Artículo científico |
| id | redalyc_44965908005 |
| language | es |
| publishDate | 2019 |
| publisher | Universidad de Costa Rica |
| spellingShingle | Morfoanatomía e histoquímica de Hypericum juniperinum (Hypericaceae) Laura Alejandra Mejía-Agudelo Biología canals histochemistry phenolic compounds translucent glands Anatomical characterization Morfoanatomía e histoquímica de Hypericum juniperinum (Hypericaceae) Laura Alejandra Mejía-Agudelo Juan Camilo Marín-Loaiza Xavier Marquínez-Casas Biología canals histochemistry phenolic compounds translucent glands Anatomical characterization Hypericum is a genus that belongs to the Hypericaceae family, with has 469 species of herbs, trees and shrubs. Two sections in particular, Brathys and Trygynobrathys are an integral part of the Paramo flora. Hypericum genus has a wide variety of metabolites that are associated with multiple pharmacological activities, and specifically in H. juniperinum antidepressant properties related to the presence of flavonoids and phenolic compounds have been reported. The objective of this research is to relate the anatomical structures with the metabolites detected by histochemical methods present in the vegetative and reproductive organs of Hypericum juniperinum collected in the Colombian Paramo. The aerial organs (stems, leaves and flowers) of Hypericum juniperinum were collected in the village Arbolocos of the municipality of Cuitiva, Boyacá (voucher code No. 589611, COL). For the anatomical analysis, the organs of H. juniperinum were processed with fixation techniques, incorporation in paraffin, cutting in microtome and staining with Astra-blue and basic fuchsin; for the histochemical analysis freehand cuts were made with specific stains for lipids, phenol compounds, alkaloids and pectins. The anatomy of the organs of H. juniperinum should be understood in the context of the living conditions of the Paramo. Leaves are highly xeromorphic as they are chartaceous, rosette-shaped, convolute and upright, with parenchyma in bistratified palisade, thickened walls, thick cuticle and epicuticular wax in both foliar epidermis, stomata on the abaxial side and with epidermal chamber. However, it has some mesomorphic characteristics such as parenchymatic bundle sheath and loose spongy parenchyma. Hypericum juniperinum has translucent glands in leaves, calyx and gynoecium, and lacks black glands typical of the Hypericum section belonging to the medicinal plant Hypericum perforatum. Histochemical analysis showed lipids in leaf canals, radial parenchyma of the stem and in some floral structures. Phenolic compounds were detected along the leaf, in the phloem of the stem and in the anthers. Hypericum juniperinum lacks alkaloids and presents pectins in the parenchyma of the sheath surrounding the vascular bundle of the leaf. The present work contributes by relating the metabolites with their distribution in the tissues of this native species. In addition, the anatomy of H. juniperinum has characteristics of the Paramo plants. Description given here could be used as a preliminary monograph of plants from the Hypericum genus with promising therapeutic properties. 2019 artículo científico 0034-7744 https://www.redalyc.org/articulo.oa?id=44965908005 https://www.redalyc.org/journal/449/44965908005/ https://www.redalyc.org/journal/449/44965908005/html/ https://www.redalyc.org/journal/449/44965908005/44965908005.epub https://www.redalyc.org/journal/449/44965908005/movil DOI 10.15517/RBT.V67I6.36072 es http://www.redalyc.org/revista.oa?id=449 Revista de Biología Tropical application/pdf Universidad de Costa Rica Revista de Biología Tropical (Costa Rica) Num.6 Vol.67 |
| title | Morfoanatomía e histoquímica de Hypericum juniperinum (Hypericaceae) |
| topic | Biología canals histochemistry phenolic compounds translucent glands Anatomical characterization |
| url | https://www.redalyc.org/articulo.oa?id=44965908005 https://www.redalyc.org/journal/449/44965908005/ https://www.redalyc.org/journal/449/44965908005/html/ https://www.redalyc.org/journal/449/44965908005/44965908005.epub https://www.redalyc.org/journal/449/44965908005/movil |