Effects of the fungus Pestalotiopsis maculans (Ascomycota: Amphisphaeriales) on the gametophytic development of the fern Lygodium venustum (Lygodiaceae)
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| Format: | Artículo científico |
| Language: | en |
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Universidad de Costa Rica
2019
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| author | Julieta Bejar |
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| contents | Effects of the fungus Pestalotiopsis maculans (Ascomycota: Amphisphaeriales) on the gametophytic development of the fern Lygodium venustum (Lygodiaceae) Julieta Bejar María Luján Luna Mónica Adriana Murace Mario Carlos Nazareno Saparrat Biología ferns Argentina leaf spots gametophyte Pathogenic fungus Lygodium Sw. is the only genus of the family Lygodiaceae, with around 25 to 40 species inhabiting mostly tropical regions worldwide. In Argentina two species develop; one of them, L. venustum, grows in the Northeast of the country. Some species of Lygodium are invasive whereas medicinal uses have been reported for some taxa. As part of a project to ascertain on the reproductive biology of native ferns in Argentina, a fungus was found during in vitro spore culture of L. venustum. Dark brown spots and eruptive pustules were also observed in fertile leaves. Thus, emerged as objectives of this work: 1) to retrieve and identify the fungus from the sporophyte and from the spores cultures, 2) to inquire on the impact of the fungus on gametophyte generation, and 3) to perform experiments with untreated spores to demonstrate that the fungus is associated to the spores collected from infested sporophytes. Phytopathological techniques were applied in order to isolate the fungus and then to achieve monosporic cultures. The morpho-biometric features of the conidia corresponded with those of Pestalotiopsis maculans (Corda) Nag Raj = Pestalotiopsis guepinii (Desm.) Steyaert (Amphisphaeriales, Ascomycota). Re-inoculation of conidia on healthy gametophytes conducted to their necrosis and death. The percentages of spore germination were initially higher in unsterilized cultures, suggesting that disinfection protocol may have affected spore germination. This is the first report of Pestalotiopsis maculans on the sporophytes of Lygodium venustum. The information that emerges from our work could be useful for the biocontrol of other species of Lygodium, considered weeds in other regions of the world. 2019 artículo científico 0034-7744 https://www.redalyc.org/articulo.oa?id=44965908029 https://www.redalyc.org/journal/449/44965908029/ https://www.redalyc.org/journal/449/44965908029/html/ https://www.redalyc.org/journal/449/44965908029/44965908029.epub https://www.redalyc.org/journal/449/44965908029/movil DOI 10.15517/RBT.V67I6.35860 en http://www.redalyc.org/revista.oa?id=449 Revista de Biología Tropical application/pdf Universidad de Costa Rica Revista de Biología Tropical (Costa Rica) Num.6 Vol.67 |
| format | Artículo científico |
| id | redalyc_44965908029 |
| institution | Redalyc |
| language | en |
| publishDate | 2019 |
| publisher | Universidad de Costa Rica |
| spellingShingle | Effects of the fungus Pestalotiopsis maculans (Ascomycota: Amphisphaeriales) on the gametophytic development of the fern Lygodium venustum (Lygodiaceae) Julieta Bejar Biología ferns Argentina leaf spots gametophyte Pathogenic fungus Effects of the fungus Pestalotiopsis maculans (Ascomycota: Amphisphaeriales) on the gametophytic development of the fern Lygodium venustum (Lygodiaceae) Julieta Bejar María Luján Luna Mónica Adriana Murace Mario Carlos Nazareno Saparrat Biología ferns Argentina leaf spots gametophyte Pathogenic fungus Lygodium Sw. is the only genus of the family Lygodiaceae, with around 25 to 40 species inhabiting mostly tropical regions worldwide. In Argentina two species develop; one of them, L. venustum, grows in the Northeast of the country. Some species of Lygodium are invasive whereas medicinal uses have been reported for some taxa. As part of a project to ascertain on the reproductive biology of native ferns in Argentina, a fungus was found during in vitro spore culture of L. venustum. Dark brown spots and eruptive pustules were also observed in fertile leaves. Thus, emerged as objectives of this work: 1) to retrieve and identify the fungus from the sporophyte and from the spores cultures, 2) to inquire on the impact of the fungus on gametophyte generation, and 3) to perform experiments with untreated spores to demonstrate that the fungus is associated to the spores collected from infested sporophytes. Phytopathological techniques were applied in order to isolate the fungus and then to achieve monosporic cultures. The morpho-biometric features of the conidia corresponded with those of Pestalotiopsis maculans (Corda) Nag Raj = Pestalotiopsis guepinii (Desm.) Steyaert (Amphisphaeriales, Ascomycota). Re-inoculation of conidia on healthy gametophytes conducted to their necrosis and death. The percentages of spore germination were initially higher in unsterilized cultures, suggesting that disinfection protocol may have affected spore germination. This is the first report of Pestalotiopsis maculans on the sporophytes of Lygodium venustum. The information that emerges from our work could be useful for the biocontrol of other species of Lygodium, considered weeds in other regions of the world. 2019 artículo científico 0034-7744 https://www.redalyc.org/articulo.oa?id=44965908029 https://www.redalyc.org/journal/449/44965908029/ https://www.redalyc.org/journal/449/44965908029/html/ https://www.redalyc.org/journal/449/44965908029/44965908029.epub https://www.redalyc.org/journal/449/44965908029/movil DOI 10.15517/RBT.V67I6.35860 en http://www.redalyc.org/revista.oa?id=449 Revista de Biología Tropical application/pdf Universidad de Costa Rica Revista de Biología Tropical (Costa Rica) Num.6 Vol.67 |
| title | Effects of the fungus Pestalotiopsis maculans (Ascomycota: Amphisphaeriales) on the gametophytic development of the fern Lygodium venustum (Lygodiaceae) |
| topic | Biología ferns Argentina leaf spots gametophyte Pathogenic fungus |
| url | https://www.redalyc.org/articulo.oa?id=44965908029 https://www.redalyc.org/journal/449/44965908029/ https://www.redalyc.org/journal/449/44965908029/html/ https://www.redalyc.org/journal/449/44965908029/44965908029.epub https://www.redalyc.org/journal/449/44965908029/movil |