Establishment of the entomopathogenic fungus Beauveria bassiana as an endophyte in Capsicum annuum and its effects on the aphid pest Myzus persicae (Homoptera: Aphididae)
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| Format: | Artículo científico |
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Universidad de Costa Rica
2020
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| author | Natalia Allegrucci |
| author_facet | Natalia Allegrucci |
| contents | Establishment of the entomopathogenic fungus Beauveria bassiana as an endophyte in Capsicum annuum and its effects on the aphid pest Myzus persicae (Homoptera: Aphididae) Natalia Allegrucci Maria-Silvana Velazquez María-Leticia Russo Maria-Florencia Vianna Camila Abarca Ana-Clara Scorsetti Biología aphid pest Endophytic fungi inoculation techniques biological control agents Introduction: Entomopathogenic fungi have been used as biological control agents of insect pests and have demonstrated a promising ability to colonize as endophytes a wide range of plant species. Objective: The aims of this study were to evaluate, in pepper plants, the most effective Beauveria bassiana inoculation techniques. In order to evaluate the presence of natural endophytes and its interaction with B. bassiana. Also to assess the effect of B. bassiana on the aphid Myzus persicae pepper leaf feeding preference and growth. Methods: Three inoculation techniques were evaluated: leaf spraying, seed immersion and root dipping. The pepper plants were inoculated with a conidia suspension adjusted to 1×108 conidia/ml. The presence of B. bassiana in the seedlings was evaluated at 7, 15 and 28 days after inoculation. Results: We found significant statistical differences between inoculation techniques. Foliar spraying showed the highest B. bassiana colonizationfrequency. The natural endophytes presented higher presence in the treatments in which B. bassiana had a low presence. This might indicate the occurrence of antifungal activity that interferes with the establishment of the entomopathogen in pepper tissues. We did not find statistical differences between inoculated and control pepper leaves with regard to the mortality and reproduction of the aphid pest M. persicae. However, the inoculation with B. bassiana showed a tendency to increase the mortality of adults and to decrease the offspring (nymphs). We did not find significant differences in the feeding preference between inoculated and control leaves, although there was a higher number of individuals on control leaves compared to those inoculated. Conclusions: The strain B. bassiana LPSC 1067 was established effectively as an endophyte in the pepper tissues. The most effective inoculation technique was leaf spraying. We did not find differences of statistical significance with regard to the mortality and reproduction of the aphid pest M. persicae. However, the inoculation with B. bassiana showed a tendency to increase the mortality of adults and to decrease the offspring (nymphs). Future studies evaluating the indirect effects of endophytic entomopathogens on both plants and herbivorous insects are needed to further explore their potential use in the management of insect pests. 2020 artículo científico 0034-7744 https://www.redalyc.org/articulo.oa?id=44966323006 https://www.redalyc.org/journal/449/44966323006/ https://www.redalyc.org/journal/449/44966323006/html/ https://www.redalyc.org/journal/449/44966323006/44966323006.epub https://www.redalyc.org/journal/449/44966323006/movil DOI 10.15517/RBT.V68I4.41218 en http://www.redalyc.org/revista.oa?id=449 Revista de Biología Tropical application/pdf Universidad de Costa Rica Revista de Biología Tropical (Costa Rica) Num.4 Vol.68 |
| format | Artículo científico |
| id | redalyc_44966323006 |
| institution | Redalyc |
| language | en |
| publishDate | 2020 |
| publisher | Universidad de Costa Rica |
| spellingShingle | Establishment of the entomopathogenic fungus Beauveria bassiana as an endophyte in Capsicum annuum and its effects on the aphid pest Myzus persicae (Homoptera: Aphididae) Natalia Allegrucci Biología aphid pest Endophytic fungi inoculation techniques biological control agents Establishment of the entomopathogenic fungus Beauveria bassiana as an endophyte in Capsicum annuum and its effects on the aphid pest Myzus persicae (Homoptera: Aphididae) Natalia Allegrucci Maria-Silvana Velazquez María-Leticia Russo Maria-Florencia Vianna Camila Abarca Ana-Clara Scorsetti Biología aphid pest Endophytic fungi inoculation techniques biological control agents Introduction: Entomopathogenic fungi have been used as biological control agents of insect pests and have demonstrated a promising ability to colonize as endophytes a wide range of plant species. Objective: The aims of this study were to evaluate, in pepper plants, the most effective Beauveria bassiana inoculation techniques. In order to evaluate the presence of natural endophytes and its interaction with B. bassiana. Also to assess the effect of B. bassiana on the aphid Myzus persicae pepper leaf feeding preference and growth. Methods: Three inoculation techniques were evaluated: leaf spraying, seed immersion and root dipping. The pepper plants were inoculated with a conidia suspension adjusted to 1×108 conidia/ml. The presence of B. bassiana in the seedlings was evaluated at 7, 15 and 28 days after inoculation. Results: We found significant statistical differences between inoculation techniques. Foliar spraying showed the highest B. bassiana colonizationfrequency. The natural endophytes presented higher presence in the treatments in which B. bassiana had a low presence. This might indicate the occurrence of antifungal activity that interferes with the establishment of the entomopathogen in pepper tissues. We did not find statistical differences between inoculated and control pepper leaves with regard to the mortality and reproduction of the aphid pest M. persicae. However, the inoculation with B. bassiana showed a tendency to increase the mortality of adults and to decrease the offspring (nymphs). We did not find significant differences in the feeding preference between inoculated and control leaves, although there was a higher number of individuals on control leaves compared to those inoculated. Conclusions: The strain B. bassiana LPSC 1067 was established effectively as an endophyte in the pepper tissues. The most effective inoculation technique was leaf spraying. We did not find differences of statistical significance with regard to the mortality and reproduction of the aphid pest M. persicae. However, the inoculation with B. bassiana showed a tendency to increase the mortality of adults and to decrease the offspring (nymphs). Future studies evaluating the indirect effects of endophytic entomopathogens on both plants and herbivorous insects are needed to further explore their potential use in the management of insect pests. 2020 artículo científico 0034-7744 https://www.redalyc.org/articulo.oa?id=44966323006 https://www.redalyc.org/journal/449/44966323006/ https://www.redalyc.org/journal/449/44966323006/html/ https://www.redalyc.org/journal/449/44966323006/44966323006.epub https://www.redalyc.org/journal/449/44966323006/movil DOI 10.15517/RBT.V68I4.41218 en http://www.redalyc.org/revista.oa?id=449 Revista de Biología Tropical application/pdf Universidad de Costa Rica Revista de Biología Tropical (Costa Rica) Num.4 Vol.68 |
| title | Establishment of the entomopathogenic fungus Beauveria bassiana as an endophyte in Capsicum annuum and its effects on the aphid pest Myzus persicae (Homoptera: Aphididae) |
| topic | Biología aphid pest Endophytic fungi inoculation techniques biological control agents |
| url | https://www.redalyc.org/articulo.oa?id=44966323006 https://www.redalyc.org/journal/449/44966323006/ https://www.redalyc.org/journal/449/44966323006/html/ https://www.redalyc.org/journal/449/44966323006/44966323006.epub https://www.redalyc.org/journal/449/44966323006/movil |