Population genetics of the fish Brycon henni (Characiformes: Bryconidae) using species-specific polymorphic microsatellite loci
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| Natura: | Artículo científico |
| Lingua: | en |
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Universidad de Costa Rica
2020
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| _version_ | 1876445096335900672 |
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| author | Ricardo M. Landínez-García |
| author_facet | Ricardo M. Landínez-García |
| contents | Population genetics of the fish Brycon henni (Characiformes: Bryconidae) using species-specific polymorphic microsatellite loci Ricardo M. Landínez-García Edna J. Márquez Biología Next sabaleta conservation Neotropical fish molecular markers Introduction: The freshwater fish Brycon henni (Characiformes: Bryconidae) is endemic to Colombia and currently considered as a “least concern” species according to the International Union for Conservation of Nature (IUCN). Objective: To develop microsatellite markers to examine population genetics in B. henni. Methods: Using a low-coverage sequencing genomic library, this study developed the first set of microsatellite loci to study the population genetics of this Neotropical species. These loci were used to evaluate the genetic diversity and structure of B. henni from three sites of the Magdalena-Cauca Basin (Colombia). Results: A set of 21 polymorphic microsatellite loci was highly informative and revealed that B. henni exhibits genetic diversity (5.143-5.619 alleles/locus, observed and expected heterozygosity = 0.461-0.645 and 0.604-0.662, respectively) and is evenly genetically structured between two tributaries of the Cauca River separated by only 30 km (F’ST = 0.093, Jost’s DEST = 0.311, P < 0.001) a finding that indicates these may be reproductively isolated groups. Conclusions: We reported a set of 21 polymorphic microsatellite loci that allowed the detection of genetic structure at local and regional scales. This population genetic structure, concordant with that found in eight congeners, is relevant when determining the risk categorization of B. henni, as well as management, conservation, and restocking programs for this species. 2020 artículo científico 0034-7744 https://www.redalyc.org/articulo.oa?id=44967847010 https://www.redalyc.org/journal/449/44967847010/ https://www.redalyc.org/journal/449/44967847010/html/ https://www.redalyc.org/journal/449/44967847010/44967847010.epub https://www.redalyc.org/journal/449/44967847010/movil DOI10.15517/RBT.V68I3.38405 en http://www.redalyc.org/revista.oa?id=449 Revista de Biología Tropical application/pdf Universidad de Costa Rica Revista de Biología Tropical (Costa Rica) Num.3 Vol.68 |
| format | Artículo científico |
| id | redalyc_44967847010 |
| institution | Redalyc |
| language | en |
| publishDate | 2020 |
| publisher | Universidad de Costa Rica |
| spellingShingle | Population genetics of the fish Brycon henni (Characiformes: Bryconidae) using species-specific polymorphic microsatellite loci Ricardo M. Landínez-García Biología Next sabaleta conservation Neotropical fish molecular markers Population genetics of the fish Brycon henni (Characiformes: Bryconidae) using species-specific polymorphic microsatellite loci Ricardo M. Landínez-García Edna J. Márquez Biología Next sabaleta conservation Neotropical fish molecular markers Introduction: The freshwater fish Brycon henni (Characiformes: Bryconidae) is endemic to Colombia and currently considered as a “least concern” species according to the International Union for Conservation of Nature (IUCN). Objective: To develop microsatellite markers to examine population genetics in B. henni. Methods: Using a low-coverage sequencing genomic library, this study developed the first set of microsatellite loci to study the population genetics of this Neotropical species. These loci were used to evaluate the genetic diversity and structure of B. henni from three sites of the Magdalena-Cauca Basin (Colombia). Results: A set of 21 polymorphic microsatellite loci was highly informative and revealed that B. henni exhibits genetic diversity (5.143-5.619 alleles/locus, observed and expected heterozygosity = 0.461-0.645 and 0.604-0.662, respectively) and is evenly genetically structured between two tributaries of the Cauca River separated by only 30 km (F’ST = 0.093, Jost’s DEST = 0.311, P < 0.001) a finding that indicates these may be reproductively isolated groups. Conclusions: We reported a set of 21 polymorphic microsatellite loci that allowed the detection of genetic structure at local and regional scales. This population genetic structure, concordant with that found in eight congeners, is relevant when determining the risk categorization of B. henni, as well as management, conservation, and restocking programs for this species. 2020 artículo científico 0034-7744 https://www.redalyc.org/articulo.oa?id=44967847010 https://www.redalyc.org/journal/449/44967847010/ https://www.redalyc.org/journal/449/44967847010/html/ https://www.redalyc.org/journal/449/44967847010/44967847010.epub https://www.redalyc.org/journal/449/44967847010/movil DOI10.15517/RBT.V68I3.38405 en http://www.redalyc.org/revista.oa?id=449 Revista de Biología Tropical application/pdf Universidad de Costa Rica Revista de Biología Tropical (Costa Rica) Num.3 Vol.68 |
| title | Population genetics of the fish Brycon henni (Characiformes: Bryconidae) using species-specific polymorphic microsatellite loci |
| topic | Biología Next sabaleta conservation Neotropical fish molecular markers |
| url | https://www.redalyc.org/articulo.oa?id=44967847010 https://www.redalyc.org/journal/449/44967847010/ https://www.redalyc.org/journal/449/44967847010/html/ https://www.redalyc.org/journal/449/44967847010/44967847010.epub https://www.redalyc.org/journal/449/44967847010/movil |