Toxicological, enzymatic, and immunochemical characterization of Bothrops asper (Serpentes: Viperidae) reference venom from Panama
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| Format: | Artículo científico |
| Sprache: | en |
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Universidad de Costa Rica
2021
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| _version_ | 1876433399929896960 |
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| author | Alina Uribe-Arjona |
| author_facet | Alina Uribe-Arjona |
| contents | Toxicological, enzymatic, and immunochemical characterization of Bothrops asper (Serpentes: Viperidae) reference venom from Panama Alina Uribe-Arjona Hildaura Acosta-de-Patiño Víctor Martínez-Cortés David Correa-Ceballos Abdiel Rodríguez Leandra Gómez-Leija Natalia Vega José-María Gutiérrez Rafael Otero-Patiño Biología venom toxicity Snakebite antivenom immunochemical characterization Introduction: It is estimated that 2 000 snakebites occur in Panama every year, 70 % of which are inflicted by Bothrops asper. Objective: To determine the biochemical and toxicologic effects and to assess the immunochemical characteristics of a reference pool of B. asper venom representative of Panama. Methods: The reference venom was prepared as a homogeneous mixture of the venoms obtained from 78 adult snakes collected in four geographic areas of Panama. Enzymatic and toxicological activities were assessed. The electrophoretic pattern was studied by SDS-PAGE. Immunoreactivity of various antivenoms was analyzed by Western blot. Results: B. asper reference venom has lethal, hemorrhagic, myotoxic, edema-forming, coagulant, defibrinating, proteinase and phospholipase A2 activities. SDS-PAGE showed the presence of protein bands with molecular weights ranging from 8 to 70 kDa, with the presence of predominant bands at ≈ 15 kDa and ≈ 30 to 66 kDa, which likely correspond to phospholipases A2 and metalloproteinases, respectively. Immunoblotting showed a high degree of recognition by various antivenoms, especially by antivenoms from Colombia and Costa Rica. Conclusions: Following recommendations by the World Health Organization, this reference venom of B. asper of Panama will become a useful tool for the preclinical evaluation of antivenoms distributed in this country. 2021 artículo científico 0034-7744 https://www.redalyc.org/articulo.oa?id=44967852011 https://www.redalyc.org/journal/449/44967852011/ https://www.redalyc.org/journal/449/44967852011/html/ https://www.redalyc.org/journal/449/44967852011/44967852011.epub https://www.redalyc.org/journal/449/44967852011/movil 10.15517/rbt.v69i1.39502 en http://www.redalyc.org/revista.oa?id=449 Revista de Biología Tropical application/pdf Universidad de Costa Rica Revista de Biología Tropical (Costa Rica) Num.1 Vol.69 |
| format | Artículo científico |
| id | redalyc_44967852011 |
| institution | Redalyc |
| language | en |
| publishDate | 2021 |
| publisher | Universidad de Costa Rica |
| spellingShingle | Toxicological, enzymatic, and immunochemical characterization of Bothrops asper (Serpentes: Viperidae) reference venom from Panama Alina Uribe-Arjona Biología venom toxicity Snakebite antivenom immunochemical characterization Toxicological, enzymatic, and immunochemical characterization of Bothrops asper (Serpentes: Viperidae) reference venom from Panama Alina Uribe-Arjona Hildaura Acosta-de-Patiño Víctor Martínez-Cortés David Correa-Ceballos Abdiel Rodríguez Leandra Gómez-Leija Natalia Vega José-María Gutiérrez Rafael Otero-Patiño Biología venom toxicity Snakebite antivenom immunochemical characterization Introduction: It is estimated that 2 000 snakebites occur in Panama every year, 70 % of which are inflicted by Bothrops asper. Objective: To determine the biochemical and toxicologic effects and to assess the immunochemical characteristics of a reference pool of B. asper venom representative of Panama. Methods: The reference venom was prepared as a homogeneous mixture of the venoms obtained from 78 adult snakes collected in four geographic areas of Panama. Enzymatic and toxicological activities were assessed. The electrophoretic pattern was studied by SDS-PAGE. Immunoreactivity of various antivenoms was analyzed by Western blot. Results: B. asper reference venom has lethal, hemorrhagic, myotoxic, edema-forming, coagulant, defibrinating, proteinase and phospholipase A2 activities. SDS-PAGE showed the presence of protein bands with molecular weights ranging from 8 to 70 kDa, with the presence of predominant bands at ≈ 15 kDa and ≈ 30 to 66 kDa, which likely correspond to phospholipases A2 and metalloproteinases, respectively. Immunoblotting showed a high degree of recognition by various antivenoms, especially by antivenoms from Colombia and Costa Rica. Conclusions: Following recommendations by the World Health Organization, this reference venom of B. asper of Panama will become a useful tool for the preclinical evaluation of antivenoms distributed in this country. 2021 artículo científico 0034-7744 https://www.redalyc.org/articulo.oa?id=44967852011 https://www.redalyc.org/journal/449/44967852011/ https://www.redalyc.org/journal/449/44967852011/html/ https://www.redalyc.org/journal/449/44967852011/44967852011.epub https://www.redalyc.org/journal/449/44967852011/movil 10.15517/rbt.v69i1.39502 en http://www.redalyc.org/revista.oa?id=449 Revista de Biología Tropical application/pdf Universidad de Costa Rica Revista de Biología Tropical (Costa Rica) Num.1 Vol.69 |
| title | Toxicological, enzymatic, and immunochemical characterization of Bothrops asper (Serpentes: Viperidae) reference venom from Panama |
| topic | Biología venom toxicity Snakebite antivenom immunochemical characterization |
| url | https://www.redalyc.org/articulo.oa?id=44967852011 https://www.redalyc.org/journal/449/44967852011/ https://www.redalyc.org/journal/449/44967852011/html/ https://www.redalyc.org/journal/449/44967852011/44967852011.epub https://www.redalyc.org/journal/449/44967852011/movil |