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| Natura: | Artículo científico |
| Lingua: | en |
| Pubblicazione: |
Universidad de Costa Rica
2021
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| Accesso online: | https://www.redalyc.org/articulo.oa?id=44969403014 https://www.redalyc.org/journal/449/44969403014/ https://www.redalyc.org/journal/449/44969403014/html/ https://www.redalyc.org/journal/449/44969403014/44969403014.epub https://www.redalyc.org/journal/449/44969403014/movil |
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Sommario:
- Action of heparin and acetylcholine modulators on the neurotoxicity of the toad Rhinella schneideri (Anura: Bufonidae) in Brazil Sandro Rostelato-Ferreira Orlando-B. Vettorazzo Natália Tribuiani Allan-P. Leal Cháriston-A. Dal-Belo Léa Rodrigues-Simioni Rafael-S. Floriano Yoko Oshima-Franco Biología reversal Amphibian poison neuromuscular blockade paratoid gland secretion chick biventer cervicis preparation Introduction: Rhinella schneideri is a toad widely distributed in South America and its poison is characterized by inducing cardiotoxicity and neurotoxicity. Objective: In this work, we investigated pharmacological strategies to attenuate the peripheral neurotoxicity induced by R. schneideri poison in avian neuromuscular preparation. Methods: The experiments were carried out using isolated chick biventer cervicis preparation subjected to field stimulation for muscle twitches recordings or exposed to acetylcholine and potassium chloride for contracture responses. Results: Poison (10 μg/ml) produced complete neuromuscular blockade in chick biventer cervicis preparation within approximately 70 min incubation (times for 50 and 90 % blockade: 15 ± 3 min and 40 ± 2 min, respectively; P < 0.05, N= 5); contracture responses to exogenous acetylcholine and KCl were unaffected by poison indicating no specificity with postsynaptic receptors or myotoxicity, respectively. Poison (10 μg/ml)-induced neuromuscular blockade was not prevented by heparin (5 and 150 IU/ml) under pre- or post-treatment conditions. Incubation at low temperature (23-25 °C) abolished the neuromuscular blockade; after raising the temperature to 37 °C, the complete neuromuscular blockade was slightly slower than that seen in preparations directly incubated at 37 °C (times for 50 and 90 % blockade: 23 ± 2 min and 60 ± 2.5 min, respectively; P < 0.05, N= 4). Neostigmine (3.3 μM) did not reverse the neuromuscular blockade in BC preparation whereas 3,4-diaminopyridine (91.6 μM) produced a partial and sustained reversal of the twitch responses (29 ± 7.8 % of maximal reversal reached in approximately 40 min incubation; P < 0.05, N= 4). Conclusions: R. schneideri poison induces potent peripheral neurotoxicity in vitro which can be partially reversible by 3,4-diaminopyridine. 2021 artículo científico 0034-7744 https://www.redalyc.org/articulo.oa?id=44969403014 https://www.redalyc.org/journal/449/44969403014/ https://www.redalyc.org/journal/449/44969403014/html/ https://www.redalyc.org/journal/449/44969403014/44969403014.epub https://www.redalyc.org/journal/449/44969403014/movil 10.15517/rbt.v69i2.44539 en http://www.redalyc.org/revista.oa?id=449 Revista de Biología Tropical application/pdf Universidad de Costa Rica Revista de Biología Tropical (Costa Rica) Num.2 Vol.69