Reverse-Transcription Loop-Mediated Isothermal Amplification and alternative protocols for lower cost, large-scale COVID-19 testing: lessons from an emerging economy
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| Natura: | Artículo científico |
| Lingua: | en |
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Universidad de Costa Rica
2022
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| _version_ | 1876490436830298112 |
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| author | Ismael Segura-Ulate |
| author_facet | Ismael Segura-Ulate |
| contents | Reverse-Transcription Loop-Mediated Isothermal Amplification and alternative protocols for lower cost, large-scale COVID-19 testing: lessons from an emerging economy Ismael Segura-Ulate Alejandro Bolívar-González Germán Madrigal-Redondo Santiago Nuñez-Corrales Andrés Gatica-Arias Biología CoV SARS saliva molecular testing Emerging economies Introduction: Most successful cases of COVID-19 pandemic mitigation and handling have relied on extensive reverse-transcription quantitative polymerase chain reaction (RT-qPCR). However, many emerging economies have struggled with current molecular testing demands due to economic, technical and technological constraints.Objective: To define a potential diagnostic protocol to increase testing capacity in current and post-pandemic conditions.Methods: We reviewed the literature, patents and commercial applications, for alternatives. Results: We found a good potential in saliva samples, viral inactivation and quick RNA extraction by heating; the use of an isothermal technology such as loop mediated isothermal amplification (LAMP) and naked eye test-result visualization by in-tube colorimetry or turbidity.Conclusions: Saliva samples with quick RNA extraction by heating and colorimetric LAMP are promising options for countries with economic and infrastructure limitations. 2022 artículo científico 0034-7744 https://www.redalyc.org/articulo.oa?id=44971236012 https://www.redalyc.org/journal/449/44971236012/ https://www.redalyc.org/journal/449/44971236012/html/ https://www.redalyc.org/journal/449/44971236012/44971236012.epub https://www.redalyc.org/journal/449/44971236012/movil 10.15517/rev.biol.trop..v70i1.47407 en http://www.redalyc.org/revista.oa?id=449 Revista de Biología Tropical application/pdf Universidad de Costa Rica Revista de Biología Tropical (Costa Rica) Num.1 Vol.70 |
| format | Artículo científico |
| id | redalyc_44971236012 |
| institution | Redalyc |
| language | en |
| publishDate | 2022 |
| publisher | Universidad de Costa Rica |
| spellingShingle | Reverse-Transcription Loop-Mediated Isothermal Amplification and alternative protocols for lower cost, large-scale COVID-19 testing: lessons from an emerging economy Ismael Segura-Ulate Biología CoV SARS saliva molecular testing Emerging economies Reverse-Transcription Loop-Mediated Isothermal Amplification and alternative protocols for lower cost, large-scale COVID-19 testing: lessons from an emerging economy Ismael Segura-Ulate Alejandro Bolívar-González Germán Madrigal-Redondo Santiago Nuñez-Corrales Andrés Gatica-Arias Biología CoV SARS saliva molecular testing Emerging economies Introduction: Most successful cases of COVID-19 pandemic mitigation and handling have relied on extensive reverse-transcription quantitative polymerase chain reaction (RT-qPCR). However, many emerging economies have struggled with current molecular testing demands due to economic, technical and technological constraints.Objective: To define a potential diagnostic protocol to increase testing capacity in current and post-pandemic conditions.Methods: We reviewed the literature, patents and commercial applications, for alternatives. Results: We found a good potential in saliva samples, viral inactivation and quick RNA extraction by heating; the use of an isothermal technology such as loop mediated isothermal amplification (LAMP) and naked eye test-result visualization by in-tube colorimetry or turbidity.Conclusions: Saliva samples with quick RNA extraction by heating and colorimetric LAMP are promising options for countries with economic and infrastructure limitations. 2022 artículo científico 0034-7744 https://www.redalyc.org/articulo.oa?id=44971236012 https://www.redalyc.org/journal/449/44971236012/ https://www.redalyc.org/journal/449/44971236012/html/ https://www.redalyc.org/journal/449/44971236012/44971236012.epub https://www.redalyc.org/journal/449/44971236012/movil 10.15517/rev.biol.trop..v70i1.47407 en http://www.redalyc.org/revista.oa?id=449 Revista de Biología Tropical application/pdf Universidad de Costa Rica Revista de Biología Tropical (Costa Rica) Num.1 Vol.70 |
| title | Reverse-Transcription Loop-Mediated Isothermal Amplification and alternative protocols for lower cost, large-scale COVID-19 testing: lessons from an emerging economy |
| topic | Biología CoV SARS saliva molecular testing Emerging economies |
| url | https://www.redalyc.org/articulo.oa?id=44971236012 https://www.redalyc.org/journal/449/44971236012/ https://www.redalyc.org/journal/449/44971236012/html/ https://www.redalyc.org/journal/449/44971236012/44971236012.epub https://www.redalyc.org/journal/449/44971236012/movil |