Anatomy and histology of the digestive tract and immunolocalization of Npy in the fish Cichlasoma dimerus (Cichliformes: Cichlidae)
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| Format: | Artículo científico |
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Universidad de Costa Rica
2022
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| _version_ | 1876447100761276416 |
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| author | Andrés Breccia |
| author_facet | Andrés Breccia |
| contents | Anatomy and histology of the digestive tract and immunolocalization of Npy in the fish Cichlasoma dimerus (Cichliformes: Cichlidae) Andrés Breccia Mónica-Alejandra Palmieri María-Paula Di-Yorio Ariadna-Gabriela Battista Paula-Gabriela Vissio Daniela-Irina Pérez-Sirkin Biología goblet cells histochemistry histomorphology immunohistochemistry neotropical cichlids Introduction: The digestive tract of Neotropical cichlids has not been deeply studied, and it is a fundamental topic for understanding fish physiology, nutrition, trophic associations, and evolution. Objective: To describe anatomically and histologically the digestive tract of the Neotropical cichlid fish Cichlasoma dimerus and to immunolocalize the orexigenic peptide (Npy) along the intestine. Methods: We euthanized 14 adult individuals and fixed the organs in Bouin´s solution; we stained 7 μm thick paraffin sections for general description and with Alcian Blue (pH = 2.5, AB) and Periodic acid-Schiff (PAS) to identify acid or neutral glycoconjugates, respectively. Additionally, we performed immunohistochemistry for Npy in 3 adult individuals. We manually counted PAS- and AB-positive cells, and Npy-immunoreactive cells per fold. Results: There is a short oesophagus, a sac-like stomach, and a tubular intestine with two loops. The oesophagus has a stratified epithelium with a high density of PAS- and AB-positive goblet cells and striated muscle fibers in the tunica muscularis. The stomach mucosa is formed by simple columnar epithelium. The intestine has a simple columnar epithelium, with brush border and interspersed PAS- and AB-positive goblet cells, and Npy-immunoreactive cells. There is an ileorectal valve in the transition between the posterior intestine and the rectum. This last gut portion has goblet cells and a thicker tunica muscularis. Conclusions: C. dimerus shares features with other Neotropical cichlids, but the goblet cells and gastric glands distribution seems to be unique for the species. To our understanding, this is the first work to describe Npy-immunoreactive cells distribution in the intestine of a Neotropical cichlid fish. 2022 artículo científico 0034-7744 https://www.redalyc.org/articulo.oa?id=44971236016 https://www.redalyc.org/journal/449/44971236016/ https://www.redalyc.org/journal/449/44971236016/html/ https://www.redalyc.org/journal/449/44971236016/44971236016.epub https://www.redalyc.org/journal/449/44971236016/movil 10.15517/rev.biol.trop..v70i1.48957 en http://www.redalyc.org/revista.oa?id=449 Revista de Biología Tropical application/pdf Universidad de Costa Rica Revista de Biología Tropical (Costa Rica) Num.1 Vol.70 |
| format | Artículo científico |
| id | redalyc_44971236016 |
| institution | Redalyc |
| language | en |
| publishDate | 2022 |
| publisher | Universidad de Costa Rica |
| spellingShingle | Anatomy and histology of the digestive tract and immunolocalization of Npy in the fish Cichlasoma dimerus (Cichliformes: Cichlidae) Andrés Breccia Biología goblet cells histochemistry histomorphology immunohistochemistry neotropical cichlids Anatomy and histology of the digestive tract and immunolocalization of Npy in the fish Cichlasoma dimerus (Cichliformes: Cichlidae) Andrés Breccia Mónica-Alejandra Palmieri María-Paula Di-Yorio Ariadna-Gabriela Battista Paula-Gabriela Vissio Daniela-Irina Pérez-Sirkin Biología goblet cells histochemistry histomorphology immunohistochemistry neotropical cichlids Introduction: The digestive tract of Neotropical cichlids has not been deeply studied, and it is a fundamental topic for understanding fish physiology, nutrition, trophic associations, and evolution. Objective: To describe anatomically and histologically the digestive tract of the Neotropical cichlid fish Cichlasoma dimerus and to immunolocalize the orexigenic peptide (Npy) along the intestine. Methods: We euthanized 14 adult individuals and fixed the organs in Bouin´s solution; we stained 7 μm thick paraffin sections for general description and with Alcian Blue (pH = 2.5, AB) and Periodic acid-Schiff (PAS) to identify acid or neutral glycoconjugates, respectively. Additionally, we performed immunohistochemistry for Npy in 3 adult individuals. We manually counted PAS- and AB-positive cells, and Npy-immunoreactive cells per fold. Results: There is a short oesophagus, a sac-like stomach, and a tubular intestine with two loops. The oesophagus has a stratified epithelium with a high density of PAS- and AB-positive goblet cells and striated muscle fibers in the tunica muscularis. The stomach mucosa is formed by simple columnar epithelium. The intestine has a simple columnar epithelium, with brush border and interspersed PAS- and AB-positive goblet cells, and Npy-immunoreactive cells. There is an ileorectal valve in the transition between the posterior intestine and the rectum. This last gut portion has goblet cells and a thicker tunica muscularis. Conclusions: C. dimerus shares features with other Neotropical cichlids, but the goblet cells and gastric glands distribution seems to be unique for the species. To our understanding, this is the first work to describe Npy-immunoreactive cells distribution in the intestine of a Neotropical cichlid fish. 2022 artículo científico 0034-7744 https://www.redalyc.org/articulo.oa?id=44971236016 https://www.redalyc.org/journal/449/44971236016/ https://www.redalyc.org/journal/449/44971236016/html/ https://www.redalyc.org/journal/449/44971236016/44971236016.epub https://www.redalyc.org/journal/449/44971236016/movil 10.15517/rev.biol.trop..v70i1.48957 en http://www.redalyc.org/revista.oa?id=449 Revista de Biología Tropical application/pdf Universidad de Costa Rica Revista de Biología Tropical (Costa Rica) Num.1 Vol.70 |
| title | Anatomy and histology of the digestive tract and immunolocalization of Npy in the fish Cichlasoma dimerus (Cichliformes: Cichlidae) |
| topic | Biología goblet cells histochemistry histomorphology immunohistochemistry neotropical cichlids |
| url | https://www.redalyc.org/articulo.oa?id=44971236016 https://www.redalyc.org/journal/449/44971236016/ https://www.redalyc.org/journal/449/44971236016/html/ https://www.redalyc.org/journal/449/44971236016/44971236016.epub https://www.redalyc.org/journal/449/44971236016/movil |