Ontogeny of digestive enzymes in larvae of the Clown Anemonefish, Amphiprion ocellaris (Perciformes: Pomacentridae)
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| Formato: | Artículo científico |
| Lenguaje: | en |
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Universidad de Costa Rica
2023
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| _version_ | 1876476651085234176 |
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| author | Gabriela Velasco-Blanco |
| author_facet | Gabriela Velasco-Blanco |
| contents | Ontogeny of digestive enzymes in larvae of the Clown Anemonefish, Amphiprion ocellaris (Perciformes: Pomacentridae) Gabriela Velasco-Blanco Carlos-Alfonso Álvarez González Maria Isabel Abdo De la Parra Luz-Estela Rodríguez-Ibarra Leonardo Ibarra-Castro Claudia I. Maytorena-Verdugo José-Natividad Arias-Jiménez Emyr-Saul Peña-Marín Biología larvae lipases proteases early ontogeny electrophoresis Introduction: The Clown anemonefish (Amphiprion ocellaris) is the most popular fish species in the marine aquarium trade; however, there is a lack of information on their digestive physiology during larval ontogeny, valuable information needed for diet design and management protocols. Objective: To characterize the early digestive enzymes of A. ocellaris larvae. Methods: We used three pools (10 larvae each) and extracted 10 samples per tank, from just before hatching to the 38th day. We analyzed the specific activity of acid and alkaline proteases, trypsin, chymotrypsin, leucine aminopeptidase and lipase; and did acid and alkaline protease zymograms. Results: We detected all measured enzymes at hatching. Acid proteases increased in activity until the 38th day. Alkaline proteases, trypsin, chymotrypsin, and leucine aminopeptidase had the same pattern, and maximum activity on the 8th day, decreasing at the 38th day. Lipase activity peaked on the 8th and 30th day. The acid zymogram had a single band, appearing on the 8th day. A total of eight alkaline proteases were revealed (154.2, 128.1, 104.0, 59.8, 53.5, 41.9, 36.5 and 25.1 KDa), with seven bands on the 1st day and all bands from the 3rd to 8th day, decreasing at two bands (41.9 and 25.1 KDa) in the 38th day. Conclusion: A. ocellaris has a functional stomach on the 8th day, and, on the 38th day, a digestive omnivore pattern with a tendency to carnivory. 2023 artículo científico 0034-7744 https://www.redalyc.org/articulo.oa?id=44975700002 https://www.redalyc.org/journal/449/44975700002/ https://www.redalyc.org/journal/449/44975700002/html/ https://www.redalyc.org/journal/449/44975700002/44975700002.epub https://www.redalyc.org/journal/449/44975700002/movil 10.15517/rev.biol.trop..v71i1.51085 en http://www.redalyc.org/revista.oa?id=449 Revista de Biología Tropical application/pdf Universidad de Costa Rica Revista de Biología Tropical (Costa Rica) Num.1 Vol.71 |
| format | Artículo científico |
| id | redalyc_44975700002 |
| institution | Redalyc |
| language | en |
| publishDate | 2023 |
| publisher | Universidad de Costa Rica |
| spellingShingle | Ontogeny of digestive enzymes in larvae of the Clown Anemonefish, Amphiprion ocellaris (Perciformes: Pomacentridae) Gabriela Velasco-Blanco Biología larvae lipases proteases early ontogeny electrophoresis Ontogeny of digestive enzymes in larvae of the Clown Anemonefish, Amphiprion ocellaris (Perciformes: Pomacentridae) Gabriela Velasco-Blanco Carlos-Alfonso Álvarez González Maria Isabel Abdo De la Parra Luz-Estela Rodríguez-Ibarra Leonardo Ibarra-Castro Claudia I. Maytorena-Verdugo José-Natividad Arias-Jiménez Emyr-Saul Peña-Marín Biología larvae lipases proteases early ontogeny electrophoresis Introduction: The Clown anemonefish (Amphiprion ocellaris) is the most popular fish species in the marine aquarium trade; however, there is a lack of information on their digestive physiology during larval ontogeny, valuable information needed for diet design and management protocols. Objective: To characterize the early digestive enzymes of A. ocellaris larvae. Methods: We used three pools (10 larvae each) and extracted 10 samples per tank, from just before hatching to the 38th day. We analyzed the specific activity of acid and alkaline proteases, trypsin, chymotrypsin, leucine aminopeptidase and lipase; and did acid and alkaline protease zymograms. Results: We detected all measured enzymes at hatching. Acid proteases increased in activity until the 38th day. Alkaline proteases, trypsin, chymotrypsin, and leucine aminopeptidase had the same pattern, and maximum activity on the 8th day, decreasing at the 38th day. Lipase activity peaked on the 8th and 30th day. The acid zymogram had a single band, appearing on the 8th day. A total of eight alkaline proteases were revealed (154.2, 128.1, 104.0, 59.8, 53.5, 41.9, 36.5 and 25.1 KDa), with seven bands on the 1st day and all bands from the 3rd to 8th day, decreasing at two bands (41.9 and 25.1 KDa) in the 38th day. Conclusion: A. ocellaris has a functional stomach on the 8th day, and, on the 38th day, a digestive omnivore pattern with a tendency to carnivory. 2023 artículo científico 0034-7744 https://www.redalyc.org/articulo.oa?id=44975700002 https://www.redalyc.org/journal/449/44975700002/ https://www.redalyc.org/journal/449/44975700002/html/ https://www.redalyc.org/journal/449/44975700002/44975700002.epub https://www.redalyc.org/journal/449/44975700002/movil 10.15517/rev.biol.trop..v71i1.51085 en http://www.redalyc.org/revista.oa?id=449 Revista de Biología Tropical application/pdf Universidad de Costa Rica Revista de Biología Tropical (Costa Rica) Num.1 Vol.71 |
| title | Ontogeny of digestive enzymes in larvae of the Clown Anemonefish, Amphiprion ocellaris (Perciformes: Pomacentridae) |
| topic | Biología larvae lipases proteases early ontogeny electrophoresis |
| url | https://www.redalyc.org/articulo.oa?id=44975700002 https://www.redalyc.org/journal/449/44975700002/ https://www.redalyc.org/journal/449/44975700002/html/ https://www.redalyc.org/journal/449/44975700002/44975700002.epub https://www.redalyc.org/journal/449/44975700002/movil |