Development and evaluation of an alternative culture medium for mass cultivation of Azospirillum brasilense C16 using sequential statistical designs
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| Format: | Artículo científico |
| Language: | en |
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Corporación Colombiana de Investigación Agropecuaria
2012
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| author | Andrés Moreno-Galván |
| author_facet | Andrés Moreno-Galván |
| contents | Development and evaluation of an alternative culture medium for mass cultivation of Azospirillum brasilense C16 using sequential statistical designs Andrés Moreno-Galván Daniel F. Rojas-Tapias Ruth Bonilla Agrociencias factorial designs factorial designs culture medium design culture medium design bioinoculant production For mass plant growth-promoting inoculant production, a high-yield culture medium is fundamental. sequential application of statistical designs was used to optimize Azospirillum brasilense c16 biomass production. six nutritional (glycerol, glutamate, mannitol, citric acid, yeast extract and K 2 HPO 4 3H 2 O) and three mineral sources (MgsO 4 7H 2 O, Fecl 3 and NaCl) were evaluated using five statistical experiments Placket-burman, factorial design, steepest ascent, response surface analysis, and mineral screening. the optimum medium composition (g L -1 ) was as follows: 28.33 glutamate, 2.92 yeast extract, 1.34 K 2 HPO 4 3H 2 O, 0.5 MgsO 4 7H 2 O and 0.02 Fecl 3 . After 24 hours of incubation, protein (32.04 μg) and dry biomass (1.51 g L -1 ) were 1.72 and 1.68 times higher than in conventional growth medium. 2012 artículo científico 0122-8706 https://www.redalyc.org/articulo.oa?id=449945033010 en http://www.redalyc.org/revista.oa?id=4499 Ciencia y Tecnología Agropecuaria application/pdf Corporación Colombiana de Investigación Agropecuaria Ciencia y Tecnología Agropecuaria (Colombia) Num.2 Vol.13 |
| format | Artículo científico |
| id | redalyc_449945033010 |
| institution | Redalyc |
| language | en |
| publishDate | 2012 |
| publisher | Corporación Colombiana de Investigación Agropecuaria |
| spellingShingle | Development and evaluation of an alternative culture medium for mass cultivation of Azospirillum brasilense C16 using sequential statistical designs Andrés Moreno-Galván Agrociencias factorial designs factorial designs culture medium design culture medium design bioinoculant production Development and evaluation of an alternative culture medium for mass cultivation of Azospirillum brasilense C16 using sequential statistical designs Andrés Moreno-Galván Daniel F. Rojas-Tapias Ruth Bonilla Agrociencias factorial designs factorial designs culture medium design culture medium design bioinoculant production For mass plant growth-promoting inoculant production, a high-yield culture medium is fundamental. sequential application of statistical designs was used to optimize Azospirillum brasilense c16 biomass production. six nutritional (glycerol, glutamate, mannitol, citric acid, yeast extract and K 2 HPO 4 3H 2 O) and three mineral sources (MgsO 4 7H 2 O, Fecl 3 and NaCl) were evaluated using five statistical experiments Placket-burman, factorial design, steepest ascent, response surface analysis, and mineral screening. the optimum medium composition (g L -1 ) was as follows: 28.33 glutamate, 2.92 yeast extract, 1.34 K 2 HPO 4 3H 2 O, 0.5 MgsO 4 7H 2 O and 0.02 Fecl 3 . After 24 hours of incubation, protein (32.04 μg) and dry biomass (1.51 g L -1 ) were 1.72 and 1.68 times higher than in conventional growth medium. 2012 artículo científico 0122-8706 https://www.redalyc.org/articulo.oa?id=449945033010 en http://www.redalyc.org/revista.oa?id=4499 Ciencia y Tecnología Agropecuaria application/pdf Corporación Colombiana de Investigación Agropecuaria Ciencia y Tecnología Agropecuaria (Colombia) Num.2 Vol.13 |
| title | Development and evaluation of an alternative culture medium for mass cultivation of Azospirillum brasilense C16 using sequential statistical designs |
| topic | Agrociencias factorial designs factorial designs culture medium design culture medium design bioinoculant production |
| url | https://www.redalyc.org/articulo.oa?id=449945033010 |