Citotoxicidad del cadmio en hepatocitos de ratón albino y sus posibles implicaciones en ambientes tropicales
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| Format: | Artículo científico |
| Langue: | es |
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Universidad de Costa Rica
2006
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| _version_ | 1876434968803016704 |
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| author | Letty Marcano |
| author_facet | Letty Marcano |
| contents | Citotoxicidad del cadmio en hepatocitos de ratón albino y sus posibles implicaciones en ambientes tropicales Letty Marcano Clarisa de R. Faría Ingrid Carruyo Xiomara Montiel Biología Cadmium histology citotoxity hepatocytes ultrastructure We analyzed phenotypic, structural and ultrastructural alterations induced by Cd+2 in hepatocytes extracted from Swiss Albino mice. Cadmium was given orally in watery solution of CdCl2 during 100 days at concentrations of 50 ppm, 100 ppm and 150 ppm. In controls, distilled water alone was used. The samples were processed with the paraffin inclusion and hematoxilin-eosin coloration techniques for light microscopy. For transmission electron microscopy we used the conventional technique. We found phenotypic (size and weight differences) and physiologic changes (muscular weakness, unrest); at the structural level we noticed loss of trabecular disposition and of lobulillar architecture, lymphocyte agglomeration, vacuolization, dilatation of sinusoid and central vein, among others. The ultrastructural study evidenced alterations coincident with those seen with light microscopy, which were accentuated with the increase of metal concentration: nucleolus with a high number of fibrillar centers (50 ppm); voluminous lipidic drops in the cytoplasm, loose endoplasmic rough reticulum, citoplasmatic vacuolization, altered lisosomes and peroxisomes (100 ppm); contracted nuclei with condensed cromatine, dilatation of intracellular space and mitochondria, and loss of fibrillar areas (150 ppm). Cadmium produces a toxic effect in the hepatic cells; the effect is more severe at higher concentration, leading to cellular necrosis. Rev. Biol. Trop. 54(2): 257-263. Epub 2006 Jun 01. 2006 artículo científico 0034-7744 https://www.redalyc.org/articulo.oa?id=4499844003 es http://www.redalyc.org/revista.oa?id=449 Revista de Biología Tropical application/pdf Universidad de Costa Rica Revista de Biología Tropical (Costa Rica) Num.2 Vol.54 |
| format | Artículo científico |
| id | redalyc_4499844003 |
| institution | Redalyc |
| language | es |
| publishDate | 2006 |
| publisher | Universidad de Costa Rica |
| spellingShingle | Citotoxicidad del cadmio en hepatocitos de ratón albino y sus posibles implicaciones en ambientes tropicales Letty Marcano Biología Cadmium histology citotoxity hepatocytes ultrastructure Citotoxicidad del cadmio en hepatocitos de ratón albino y sus posibles implicaciones en ambientes tropicales Letty Marcano Clarisa de R. Faría Ingrid Carruyo Xiomara Montiel Biología Cadmium histology citotoxity hepatocytes ultrastructure We analyzed phenotypic, structural and ultrastructural alterations induced by Cd+2 in hepatocytes extracted from Swiss Albino mice. Cadmium was given orally in watery solution of CdCl2 during 100 days at concentrations of 50 ppm, 100 ppm and 150 ppm. In controls, distilled water alone was used. The samples were processed with the paraffin inclusion and hematoxilin-eosin coloration techniques for light microscopy. For transmission electron microscopy we used the conventional technique. We found phenotypic (size and weight differences) and physiologic changes (muscular weakness, unrest); at the structural level we noticed loss of trabecular disposition and of lobulillar architecture, lymphocyte agglomeration, vacuolization, dilatation of sinusoid and central vein, among others. The ultrastructural study evidenced alterations coincident with those seen with light microscopy, which were accentuated with the increase of metal concentration: nucleolus with a high number of fibrillar centers (50 ppm); voluminous lipidic drops in the cytoplasm, loose endoplasmic rough reticulum, citoplasmatic vacuolization, altered lisosomes and peroxisomes (100 ppm); contracted nuclei with condensed cromatine, dilatation of intracellular space and mitochondria, and loss of fibrillar areas (150 ppm). Cadmium produces a toxic effect in the hepatic cells; the effect is more severe at higher concentration, leading to cellular necrosis. Rev. Biol. Trop. 54(2): 257-263. Epub 2006 Jun 01. 2006 artículo científico 0034-7744 https://www.redalyc.org/articulo.oa?id=4499844003 es http://www.redalyc.org/revista.oa?id=449 Revista de Biología Tropical application/pdf Universidad de Costa Rica Revista de Biología Tropical (Costa Rica) Num.2 Vol.54 |
| title | Citotoxicidad del cadmio en hepatocitos de ratón albino y sus posibles implicaciones en ambientes tropicales |
| topic | Biología Cadmium histology citotoxity hepatocytes ultrastructure |
| url | https://www.redalyc.org/articulo.oa?id=4499844003 |