Simultaneous enumeration of Phaeodactylum tricornutum (MLB292) and bacteria growing in mixed communities
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| Natura: | Artículo científico |
| Lingua: | en |
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Pontificia Universidad Católica de Valparaíso
2005
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| author | Katia Soto |
| author_facet | Katia Soto |
| contents | Simultaneous enumeration of Phaeodactylum tricornutum (MLB292) and bacteria growing in mixed communities Katia Soto Marcela Zahr Gloria Collantes Juan Kuznar Ciencias de la Tierra growth bacteria enumeration epifluorescence microscopic algae Diatoms play a decisive role in global primary production, have a key role in the marine food web, andserve as food for the industrial culture of aquatic animals. In addition, species of diatoms have been chosen as tools formeasuring sea water quality and for bioassay procedures. Among them, Phaeodactylum tricornutum (Bohlin) Lewin,1958, represents a good biological model due to its ease of handling and also to its physiological, genetic and growthcharacteristics. We investigated the use of the Epifluorescence Microscopy (EPM) to visualize and enumerate P.tricornutum cells and bacteria in cultures containing both kinds of microorganisms. The cell suspension was filtered,stained with SYBR Green and analyzed by EPM. P. tricornutum can be enumerated using EPM with comparableaccuracy as using a hemocytometer. The same kind of growth curve is obtained with either method. EPM is anadvantageous alternative to the classic light microscope counting method; it allows the simultaneous enumeration of P.tricornutum and bacteria in a single sample, measuring red autofluorescence and green fluorescence respectively.Moreover, the methodology is suitable for dynamic studies performed to determine the growth dependence between P.tricornutum and bacteria. Another advantage of this technique is that different shapes, sizes and physiological stagesof the microbiological communities can be observed. 2005 artículo científico 0716-1069 https://www.redalyc.org/articulo.oa?id=45633202 en http://www.redalyc.org/revista.oa?id=456 Investigaciones Marinas application/pdf Pontificia Universidad Católica de Valparaíso Investigaciones Marinas (Chile) Num.2 Vol.33 |
| format | Artículo científico |
| id | redalyc_45633202 |
| institution | Redalyc |
| language | en |
| publishDate | 2005 |
| publisher | Pontificia Universidad Católica de Valparaíso |
| spellingShingle | Simultaneous enumeration of Phaeodactylum tricornutum (MLB292) and bacteria growing in mixed communities Katia Soto Ciencias de la Tierra growth bacteria enumeration epifluorescence microscopic algae Simultaneous enumeration of Phaeodactylum tricornutum (MLB292) and bacteria growing in mixed communities Katia Soto Marcela Zahr Gloria Collantes Juan Kuznar Ciencias de la Tierra growth bacteria enumeration epifluorescence microscopic algae Diatoms play a decisive role in global primary production, have a key role in the marine food web, andserve as food for the industrial culture of aquatic animals. In addition, species of diatoms have been chosen as tools formeasuring sea water quality and for bioassay procedures. Among them, Phaeodactylum tricornutum (Bohlin) Lewin,1958, represents a good biological model due to its ease of handling and also to its physiological, genetic and growthcharacteristics. We investigated the use of the Epifluorescence Microscopy (EPM) to visualize and enumerate P.tricornutum cells and bacteria in cultures containing both kinds of microorganisms. The cell suspension was filtered,stained with SYBR Green and analyzed by EPM. P. tricornutum can be enumerated using EPM with comparableaccuracy as using a hemocytometer. The same kind of growth curve is obtained with either method. EPM is anadvantageous alternative to the classic light microscope counting method; it allows the simultaneous enumeration of P.tricornutum and bacteria in a single sample, measuring red autofluorescence and green fluorescence respectively.Moreover, the methodology is suitable for dynamic studies performed to determine the growth dependence between P.tricornutum and bacteria. Another advantage of this technique is that different shapes, sizes and physiological stagesof the microbiological communities can be observed. 2005 artículo científico 0716-1069 https://www.redalyc.org/articulo.oa?id=45633202 en http://www.redalyc.org/revista.oa?id=456 Investigaciones Marinas application/pdf Pontificia Universidad Católica de Valparaíso Investigaciones Marinas (Chile) Num.2 Vol.33 |
| title | Simultaneous enumeration of Phaeodactylum tricornutum (MLB292) and bacteria growing in mixed communities |
| topic | Ciencias de la Tierra growth bacteria enumeration epifluorescence microscopic algae |
| url | https://www.redalyc.org/articulo.oa?id=45633202 |