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| Format: | Artículo científico |
| Language: | en |
| Published: |
Pontificia Universidad Católica de Valparaíso
2005
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| Online Access: | https://www.redalyc.org/articulo.oa?id=45633206 |
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Table of Contents:
- Determination site of vitellogenin synthesis in freshwater crayfish Cherax quadricarinatus at different maturation stages females Vania Serrano-Pinto María Guadalupe Carrisoza-Valenzuela Martín Ramírez-Orozco Ciencias de la Tierra crayfish vitellogenesis mRNA expression Cherax quadricarinatus vitellogenin synthesis The objective of this study was to determine the site of vitellogenin (Vg) synthesis at different stages offemale maturation of freshwater crayfish Cherax quadricarinatus. PCR products of 1,100 bp and 900 bp were generatedfrom genomic DNA in the first case, and hepatopancreas and ovary cDNAs in the second case. Results from RT-PCRanalyses showed that the mRNA encoding the 3´ end of the Vg cDNA was present in the hepatopancreas from secondaryvitellogenesisat first maturation, previously spawner and ovigerous females. The Vg mRNA was present simultaneouslyin the ovary from secondary-vitellogenesis at first maturation only, but was not detected at previously spawning andovigerous females. This study provided evidence that the ovary plays a significant role in the production of this majoregg yolk protein, but only in some stage of the vitellogenesis cycle. 2005 artículo científico 0716-1069 https://www.redalyc.org/articulo.oa?id=45633206 en http://www.redalyc.org/revista.oa?id=456 Investigaciones Marinas application/pdf Pontificia Universidad Católica de Valparaíso Investigaciones Marinas (Chile) Num.2 Vol.33