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| Format: | Artículo científico |
| Language: | en |
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Universidade Federal de Santa Maria
2009
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| Online Access: | https://www.redalyc.org/articulo.oa?id=467546321010 |
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Table of Contents:
- Ultraviolet and microwave radiation in Trichoderma viride isolates Neiva Braun Lilian Castilho dos Santos Solange Bosio Tedesco Juçara Teresinha Paranhos Antonio Carlos Ferreira da Silva Estudios Ambientales Mutants benomyl radiation trichoderma This paper aimed to determine the exposition time close to 5% of survival of Trichoderma viride (TSM) under ultraviolet light, and TSM and IT isolates of Trichoderma viride to microwave radiation, in order to obtain the mutants resistant to benomyl fungicide. This survival was studied from the irradiation of conidia at different exposition times. 0.1mL aliquots of conidia suspension from the isolates were irradiated at time of 5% of survival and incubated in PDA medium supplemented with doses of 0; 1.0; 5.0; and 10 m g.mL -1 of benomyl. The results showed that the exposition time of the TSM isolate to ultraviolet radiation was about two minutes and for microwave radiation the time was eight seconds for both TSM and IT isolates. Conidia from IT isolate were more resistant to microwave radiation than the TSM isolate. Eight resistant mutants to 5.0 m g.mL -1 benomyl fungicide were obtained from the TSM isolate exposed to ultraviolet radiation, both according to the resistance to the fungicide and the antagonism in vitro to phytopathogens, though with different colors of mycelia. Colonies of mutants resistant to benomyl with the use of microwave radiation were not observed. 2009 artículo científico 0100-8307 https://www.redalyc.org/articulo.oa?id=467546321010 en http://www.redalyc.org/revista.oa?id=4675 Ciência e Natura application/pdf Universidade Federal de Santa Maria Ciência e Natura (Brasil) Num.2 Vol.31