Physico-chemical characterization of collagen scaffolds for tissue engineering

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Main Author: B.H. León-Mancilla
Format: Artículo científico
Language:en
Published: Universidad Nacional Autónoma de México 2016
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author B.H. León-Mancilla
author_facet B.H. León-Mancilla
contents Physico-chemical characterization of collagen scaffolds for tissue engineering B.H. León-Mancilla M.A. Araiza-Téllez J.O. Flores-Flores M.C. Piña-Barba Ingeniería Bone matrix Biomaterials Tissue engineering Collagen scaffolds The objective was to research the physical and chemical properties of collagen scaffolds (CS) obtained from bone matrix Nukbone ® subject to a demineralization process using hydrochloric acid. The CS samples were characterized by optical and scanning electron microscopy, elemental chemical analysis, X-ray diffraction, spectroscopy Infrared, thermal analysis, differential scanning calorimetry and nitrogen adsorption. The microanalysis were used to set the macro- and microstructures of CS. They showed that the CS retained the morphology of Nukbone ® with interconnected pores and their size between 100 and 500 m, and it is composed of 20% by weight of HA and the rest is collagen type I. By infrared spectroscopy the functional groups of collagen type I (amide A – 3285, B – 2917, I – 1633, II – 1553 and III – 1239 cm − 1 ) were identified. By thermal analysis it was determined that the phenomenon of denaturation of the collagen type I began in the range of 75–85 ◦ C and burned above 200 ◦ C. 2016 artículo científico 1665-6423 https://www.redalyc.org/articulo.oa?id=47444851007 en http://www.redalyc.org/revista.oa?id=474 Journal of Applied Research and Technology application/pdf Universidad Nacional Autónoma de México Journal of Applied Research and Technology (México) Num.1 Vol.14
format Artículo científico
id redalyc_47444851007
institution Redalyc
language en
publishDate 2016
publisher Universidad Nacional Autónoma de México
spellingShingle Physico-chemical characterization of collagen scaffolds for tissue engineering
B.H. León-Mancilla
Ingeniería
Bone matrix
Biomaterials
Tissue engineering
Collagen scaffolds
Physico-chemical characterization of collagen scaffolds for tissue engineering B.H. León-Mancilla M.A. Araiza-Téllez J.O. Flores-Flores M.C. Piña-Barba Ingeniería Bone matrix Biomaterials Tissue engineering Collagen scaffolds The objective was to research the physical and chemical properties of collagen scaffolds (CS) obtained from bone matrix Nukbone ® subject to a demineralization process using hydrochloric acid. The CS samples were characterized by optical and scanning electron microscopy, elemental chemical analysis, X-ray diffraction, spectroscopy Infrared, thermal analysis, differential scanning calorimetry and nitrogen adsorption. The microanalysis were used to set the macro- and microstructures of CS. They showed that the CS retained the morphology of Nukbone ® with interconnected pores and their size between 100 and 500 m, and it is composed of 20% by weight of HA and the rest is collagen type I. By infrared spectroscopy the functional groups of collagen type I (amide A – 3285, B – 2917, I – 1633, II – 1553 and III – 1239 cm − 1 ) were identified. By thermal analysis it was determined that the phenomenon of denaturation of the collagen type I began in the range of 75–85 ◦ C and burned above 200 ◦ C. 2016 artículo científico 1665-6423 https://www.redalyc.org/articulo.oa?id=47444851007 en http://www.redalyc.org/revista.oa?id=474 Journal of Applied Research and Technology application/pdf Universidad Nacional Autónoma de México Journal of Applied Research and Technology (México) Num.1 Vol.14
title Physico-chemical characterization of collagen scaffolds for tissue engineering
topic Ingeniería
Bone matrix
Biomaterials
Tissue engineering
Collagen scaffolds
url https://www.redalyc.org/articulo.oa?id=47444851007