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Main Author:	Lorena Morales
Format:	Artículo científico
Language:	en
Published:	Sociedad Química de México 2012
Subjects:	Multidisciplinaria (Ciencias Naturales y Exactas) purification pyrophosphate Phaseolus vulgaris Alkaline phosphatase phosphate deficiency
Online Access:	https://www.redalyc.org/articulo.oa?id=47523284013
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Purification and Characterization of an Alkaline Phosphatase Induced by Phosphorus Starvation in Common Bean (Phaseolus vulgaris L.) Roots Lorena Morales Natalia Gutiérrez Vanessa Maya Carmen Parra Eleazar Martínez-Barajas Patricia Coello Multidisciplinaria (Ciencias Naturales y Exactas) purification pyrophosphate Phaseolus vulgaris Alkaline phosphatase phosphate deficiency Two phosphatase isoforms from roots of the common bean(Phaseolus vulgaris L.) showed an increase in activity in responseto phosphate deficiency. One of them (APIII) was chosen for further purification through ionic exchange chromatography and preparative electrophoresis. The estimated molecular mass of APIII was 35 kDa by both SDS-PAGE and gel filtration analyses, suggesting a monomeric form of the active enzyme. The phosphatase was classified as an alkaline phosphatase based on the requirement of pH 8 for optimum catalysis. It not only exhibited broad substrate specificity, with the most activity against pyrophosphate, but also effectively catalyzed the hydrolysis of polyphosphate, glucose-1-phosphate and phosphoenolpyruvate. Activity was completely inhibited by molybdate, vanadate and phosphate but was only partially inhibited by fluoride. Although divalent cations were not essential for the pyrophosphatase activity of this enzyme, the hydrolysis of pyrophosphate increased substantially in the presence of Mg2+. 2012 artículo científico 1870-249X https://www.redalyc.org/articulo.oa?id=47523284013 en http://www.redalyc.org/revista.oa?id=475 Journal of the Mexican Chemical Society application/pdf Sociedad Química de México Journal of the Mexican Chemical Society (México) Num.1 Vol.56

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