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| Format: | Artículo científico |
| Language: | en |
| Published: |
Sociedad Química de México
2013
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| Online Access: | https://www.redalyc.org/articulo.oa?id=47527412010 |
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Table of Contents:
- Behavior of Two and Three Electrode Configuration and Different Mediators in Working Electrode on Development of Disposable Screen-Printing Biosensors for Determination of Free Cholesterol Mónica Hernández-Cruz Carlos Andrés Galán-Vidal Giaan Arturo Álvarez-Romero María Teresa Ramírez-Silva María Elena Páez-Hernández José Luis González-Vidal Multidisciplinaria (Ciencias Naturales y Exactas) mediator transducer Cholesterol screen printing amperometric biosensor In the present work the development of an amperometric transducer in order to build a free cholesterol biosensor in planar configuration is reported. The one single use disposable biosensors were constructed by screen printing process which is compatible with automated methodologies of production. Based in the incorporation of tetracyanoquinodimethane (TCNQ), Prussian blue (PB) or ferrite (Fe3O4) as mediator or electrocatalytic agent, three types of elec- trochemical transducers were evaluated. The cholesterol biosensors require a sample volume of 7.2 μL, exhibits good reproducibility and selectivity and cover a lineal answer of 2-16 mM with detection limits from 0.3 to 1.6 mM. The characteristics of biosensors are satisfactory for the decentralized analysis of the lipid in blood since enclose the range of clinical interest (3.5-6.5 mM). 2013 artículo científico 1870-249X https://www.redalyc.org/articulo.oa?id=47527412010 en http://www.redalyc.org/revista.oa?id=475 Journal of the Mexican Chemical Society application/pdf Sociedad Química de México Journal of the Mexican Chemical Society (México) Num.1 Vol.57