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| Format: | Artículo científico |
| Sprache: | en |
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Sociedad Química de México
2013
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| Online-Zugang: | https://www.redalyc.org/articulo.oa?id=47529964005 |
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- Binding of Oxovanadium(IV) Complexes to Blood Serum Albumins Enoch Cobbina Sameena Mehtab Isabel Correia Gisela Gonçalves Isabel Tomaz Isabel Cavaco Tamás Jakusch Eva Enyedi Tamás Kiss João Costa Pessoa Multidisciplinaria (Ciencias Naturales y Exactas) maltol Electron Para Oxovanadium(IV) Circular Dichroism magnetic Resonance In this work the binding of V IV O 2+ and V IV O-complexes to serum albumins {human serum albumin (HSA), bovine serum albumin (BSA) and porcine serum albumin (PSA)} are studied using circular dichroism (CD), electron paramagnetic resonance (EPR) and visible absorption spectroscopy. The results confirm previous findings that V IV O 2+ occupies at least two types of binding sites on albumin: ‘the strong vanadium binding site’ (designated by VBS1) and ‘the weak vanadium binding sites’ (designated by VBS2). VBS1 binds 1 mol equivalent of V IV O 2+ . On the other hand VBS2 correspond to binding of several mol equivalents of V IV O, and studies done with PSA in the presence of excess Zn II ions indicate that VSB2 corresponds to two distinct types of sites. The hyperfine coupling constant A z for V IV O 2+ binding at VBS2 on HSA and BSA are all very similar (~168 × 10 - 4 cm - 1 ) but differ slightly on PSA (~166 × 10 -4 cm - 1 ) due to differences in the binding sets. When (V IV O)-HSA systems are titrated with maltol ternary species of (maltol) m (V IV O) m HSA and (maltol) 2m (V IV O) m HSA stoichiometry form which are clearly distinguishable from the binary (V IV O)-HSA system by the type and intensity of the CD spectra recorded. Changes are also observable in the intensity of the X-band EPR spectra, but not much in the hyperfine coupling constants A z , which are all in the range 166-167 × 10 - 4 cm - 1 . The results further demonstrate that the presence of maltol may enhance the binding of V IV O to albumin. 2013 artículo científico 1870-249X https://www.redalyc.org/articulo.oa?id=47529964005 en http://www.redalyc.org/revista.oa?id=475 Journal of the Mexican Chemical Society application/pdf Sociedad Química de México Journal of the Mexican Chemical Society (México) Num.3 Vol.57