Monoenzymatic Lipase Potentiometric Biosensor for the Food Analysis Based on a pH Sensitive Graphite-epoxy Composite as Transducer
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| Natura: | Artículo científico |
| Lingua: | en |
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Sociedad Química de México
2015
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| _version_ | 1876483032413634560 |
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| author | Juan Carlos Escamilla-Mejía |
| author_facet | Juan Carlos Escamilla-Mejía |
| contents | Monoenzymatic Lipase Potentiometric Biosensor for the Food Analysis Based on a pH Sensitive Graphite-epoxy Composite as Transducer Juan Carlos Escamilla-Mejía José Antonio Rodríguez Giaan Arturo Álvarez-Romero Carlos Andrés Galán-Vidal Multidisciplinaria (Ciencias Naturales y Exactas) Graphite Triglycerides Food Analysis epoxy Composite Arabic Gum Extraction In this paper the development of a potentiometric biosen-sor based on the use of the enzyme lipase immobilized in a Nafion membrane on a graphite-epoxy transducer is reported. This device has been used to quantify triglycerides in food samples from the aque-ous extracts obtained by emulsion with Arabic gum. The proposed methodology does not present significant differences with the spec-trophotometric determination used as contrast. This work constitutes the first report of a potentiometric biosensor based on the detection of changes of pH using a graphite-epoxy composite transducer and the first potentiometric triglyceride biosensor which is not based in ISFET transducers. The methodology designed is a simple and inexpensive alternative that minimizes the use of organic solvents without affecting the precision and accuracy of the analysis in complex food samples such as butter, chips and pastries. 2015 artículo científico 1870-249X https://www.redalyc.org/articulo.oa?id=47539242004 en http://www.redalyc.org/revista.oa?id=475 Journal of the Mexican Chemical Society application/pdf Sociedad Química de México Journal of the Mexican Chemical Society (México) Num.1 Vol.59 |
| format | Artículo científico |
| id | redalyc_47539242004 |
| institution | Redalyc |
| language | en |
| publishDate | 2015 |
| publisher | Sociedad Química de México |
| spellingShingle | Monoenzymatic Lipase Potentiometric Biosensor for the Food Analysis Based on a pH Sensitive Graphite-epoxy Composite as Transducer Juan Carlos Escamilla-Mejía Multidisciplinaria (Ciencias Naturales y Exactas) Graphite Triglycerides Food Analysis epoxy Composite Arabic Gum Extraction Monoenzymatic Lipase Potentiometric Biosensor for the Food Analysis Based on a pH Sensitive Graphite-epoxy Composite as Transducer Juan Carlos Escamilla-Mejía José Antonio Rodríguez Giaan Arturo Álvarez-Romero Carlos Andrés Galán-Vidal Multidisciplinaria (Ciencias Naturales y Exactas) Graphite Triglycerides Food Analysis epoxy Composite Arabic Gum Extraction In this paper the development of a potentiometric biosen-sor based on the use of the enzyme lipase immobilized in a Nafion membrane on a graphite-epoxy transducer is reported. This device has been used to quantify triglycerides in food samples from the aque-ous extracts obtained by emulsion with Arabic gum. The proposed methodology does not present significant differences with the spec-trophotometric determination used as contrast. This work constitutes the first report of a potentiometric biosensor based on the detection of changes of pH using a graphite-epoxy composite transducer and the first potentiometric triglyceride biosensor which is not based in ISFET transducers. The methodology designed is a simple and inexpensive alternative that minimizes the use of organic solvents without affecting the precision and accuracy of the analysis in complex food samples such as butter, chips and pastries. 2015 artículo científico 1870-249X https://www.redalyc.org/articulo.oa?id=47539242004 en http://www.redalyc.org/revista.oa?id=475 Journal of the Mexican Chemical Society application/pdf Sociedad Química de México Journal of the Mexican Chemical Society (México) Num.1 Vol.59 |
| title | Monoenzymatic Lipase Potentiometric Biosensor for the Food Analysis Based on a pH Sensitive Graphite-epoxy Composite as Transducer |
| topic | Multidisciplinaria (Ciencias Naturales y Exactas) Graphite Triglycerides Food Analysis epoxy Composite Arabic Gum Extraction |
| url | https://www.redalyc.org/articulo.oa?id=47539242004 |