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| Natura: | Artículo científico |
| Lingua: | en |
| Pubblicazione: |
Sociedad Química de México
2015
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| Accesso online: | https://www.redalyc.org/articulo.oa?id=47545630004 |
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Sommario:
- Label-free Electrochemical Immunosensors for Viruses and Antibodies Detection-Review Hanna Radecka Jerzy Radecki Multidisciplinaria (Ciencias Naturales y Exactas) natural matrixes viruses detection antibodies detection electrode modification redox active monolayers This article reviews the electrochemical immunosensors developed by the successive modification of gold as well as glassy carbon electrodes. Antibody or antigen fragments have been applied as the sensing elements. The complex between virions and specific antibody adsorbing on a surface of an electrode forms an insulating layer. This phenomenon, which is a base of ion – channel mimetic type of immunosensors, can be monitored by Osteryoung square-wave voltammetry (OSWV) and electrochemical impedance spectroscopy (EIS) in the presence of [Fe(CN)6]3-/4- as a redox marker. Another type of immunosensors are based on redox active layers incorporated di-pyrromethene -Cu(II) or phenanthroline – Epoxy - Fe(III) complexes. Changes of electrochemical parameters of redox centres upon target analyte binding are the basis of analytical signal generation. Both type of immunosensors displayed better sensitivity in comparison to ELISA as well as being very selective. The matrix has no influence on the immunosensors performance. These devices could be recommended for the direct electrochemical detection of viruses as well as antibodies in physiological samples. 2015 artículo científico 1870-249X https://www.redalyc.org/articulo.oa?id=47545630004 en http://www.redalyc.org/revista.oa?id=475 Journal of the Mexican Chemical Society application/pdf Sociedad Química de México Journal of the Mexican Chemical Society (México) Num.4 Vol.59