Compositional and Conformational Analysis of Sperm Nucleus Basic Proteins from the Bivalve Mollusk Crassostrea virginica
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| Natura: | Artículo científico |
| Lingua: | en |
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Sociedad Química de México
2002
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| _version_ | 1876432703507660800 |
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| author | M. Zayil Salazar Campos |
| author_facet | M. Zayil Salazar Campos |
| contents | Compositional and Conformational Analysis of Sperm Nucleus Basic Proteins from the Bivalve Mollusk Crassostrea virginica M. Zayil Salazar Campos A. Eneas Chavelas Adame Roberto Arreguín Espinosa Enrique García Hernández Multidisciplinaria (Ciencias Naturales y Exactas) histones composition purification circular dichroism Crassostrea virginica We report a study on the basic proteins from sperm nucleusof the bivalve mollusk Crassostrea virginica. This is the first studyin its type reported for this specie of wide distribution and high commercialinterest. The electrophoretic behavior of total basic proteinswas obtained, revealing the characteristic composition of O-group.Using perchloric acid extraction followed by reverse-phase high performanceliquid chromatography, a germinal histone H1-2 proteinwas isolated. An analysis of the circular dichroism spectrum of thisprotein revealed the lack of regular elements of secondary structure.The H1-2 protein was subjected to a progressive thermal perturbation,observing no abrupt CD-signal change in the scanned temperaturerange; in contrast, a continuous and significant diminution ofoptical activity was observed. Overall, these observations indicatethat at room temperature the germinal histone is a non-compact intermediatethat preserves some secondary structure, and which transitsto a more unfolded state upon temperature increase. 2002 artículo científico 1870-249X https://www.redalyc.org/articulo.oa?id=47546305 en http://www.redalyc.org/revista.oa?id=475 Journal of the Mexican Chemical Society application/pdf Sociedad Química de México Journal of the Mexican Chemical Society (México) Num.3 Vol.46 |
| format | Artículo científico |
| id | redalyc_47546305 |
| institution | Redalyc |
| language | en |
| publishDate | 2002 |
| publisher | Sociedad Química de México |
| spellingShingle | Compositional and Conformational Analysis of Sperm Nucleus Basic Proteins from the Bivalve Mollusk Crassostrea virginica M. Zayil Salazar Campos Multidisciplinaria (Ciencias Naturales y Exactas) histones composition purification circular dichroism Crassostrea virginica Compositional and Conformational Analysis of Sperm Nucleus Basic Proteins from the Bivalve Mollusk Crassostrea virginica M. Zayil Salazar Campos A. Eneas Chavelas Adame Roberto Arreguín Espinosa Enrique García Hernández Multidisciplinaria (Ciencias Naturales y Exactas) histones composition purification circular dichroism Crassostrea virginica We report a study on the basic proteins from sperm nucleusof the bivalve mollusk Crassostrea virginica. This is the first studyin its type reported for this specie of wide distribution and high commercialinterest. The electrophoretic behavior of total basic proteinswas obtained, revealing the characteristic composition of O-group.Using perchloric acid extraction followed by reverse-phase high performanceliquid chromatography, a germinal histone H1-2 proteinwas isolated. An analysis of the circular dichroism spectrum of thisprotein revealed the lack of regular elements of secondary structure.The H1-2 protein was subjected to a progressive thermal perturbation,observing no abrupt CD-signal change in the scanned temperaturerange; in contrast, a continuous and significant diminution ofoptical activity was observed. Overall, these observations indicatethat at room temperature the germinal histone is a non-compact intermediatethat preserves some secondary structure, and which transitsto a more unfolded state upon temperature increase. 2002 artículo científico 1870-249X https://www.redalyc.org/articulo.oa?id=47546305 en http://www.redalyc.org/revista.oa?id=475 Journal of the Mexican Chemical Society application/pdf Sociedad Química de México Journal of the Mexican Chemical Society (México) Num.3 Vol.46 |
| title | Compositional and Conformational Analysis of Sperm Nucleus Basic Proteins from the Bivalve Mollusk Crassostrea virginica |
| topic | Multidisciplinaria (Ciencias Naturales y Exactas) histones composition purification circular dichroism Crassostrea virginica |
| url | https://www.redalyc.org/articulo.oa?id=47546305 |