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| Format: | Artículo científico |
| Language: | en |
| Published: |
Sociedad Química de México
2004
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| Online Access: | https://www.redalyc.org/articulo.oa?id=47548422 |
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Table of Contents:
- Simultaneous spectrophotometric determination of isoperoxisomicine A1 and peroxisomicine A1 using partial least square regression type 1 (PLS-1) Alfredo Piñeyro López Ma. de la Luz Salazar Pedro Luis López de Alba Noemí Waksman de Torres Multidisciplinaria (Ciencias Naturales y Exactas) PLS peroxisomicine spectrophotometry isoperoxisomicine multivariate calibration Multivariate calibration with regression by partial leastsquares (PLS-1) is herein proposed as an alternative method for thespectrophotometric quantification of isoperoxisomicine A1 in peroxisomicineA1 batches. In order to minimize the optimal factors necessaryto obtain the calibration matrix, different parameters were evaluated.The adequate selection of the spectral regions proved to beimportant on the number of factors. In order to simultaneously quantifyboth analytes, the spectral region between 252 and 340 nm wasselected. Recoveries for isoPA1 and PA1 were 97% and 103%respectively. The developed method was applied to two batches ofPA1; the amount of isoPA1 found, showed no significant differenceswith those found by means of 1H RMN. 2004 artículo científico 1870-249X https://www.redalyc.org/articulo.oa?id=47548422 en http://www.redalyc.org/revista.oa?id=475 Journal of the Mexican Chemical Society application/pdf Sociedad Química de México Journal of the Mexican Chemical Society (México) Num.4 Vol.48