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| Format: | Artículo científico |
| Langue: | en |
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Sociedad Química de México
2024
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| Accès en ligne: | https://www.redalyc.org/articulo.oa?id=47581848002 https://www.redalyc.org/journal/475/47581848002/ https://www.redalyc.org/journal/475/47581848002/html/ https://www.redalyc.org/journal/475/47581848002/47581848002.epub https://www.redalyc.org/journal/475/47581848002/movil |
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Table des matières:
- Determination of Sodium, Potassium, Calcium and Magnesium in Urine, Using Microwave Plasma - Atomic Emission Spectrometry and Multi-Energy Calibration Tania Lizeth Espinoza Cruz Kazimierz Wrobel Eunice Yanez Barrientos Alma Rosa Corrales Escobosa Ma. Eugenia Garay-Sevilla Francisco Javier Acevedo-Aguilar Katarzyna Wrobel Multidisciplinaria (Ciencias Naturales y Exactas) AES) multi urine diabetes Microwave plasma Microwave plasma - atomic emission spectrometry with multi-energy calibration (MP-AES-MEC) was used for the determination of four major elements in urine. In the family of atomic emission spectrometry, the distinctive features of MP-AES are: (i) nitrogen-based toroidal-shape plasma; (ii) good plasma tolerance to total solids; (iii) exceptionally low operating cost. On the other hand, due to relatively low plasma temperature, this technique is susceptible to spectral interferences and sample-to-sample fluctuating baseline is typical limitation, if previous acid digestion is not performed. MEC is a non-conventional quantification method, not requiring baseline correction and it has been selected in this work to achieve reliable determination of Na, K, Ca and Mg in simply diluted urine. The principle underlying MEC is the proportionality between signal intensity and analyte concentration, occurring at any emission line for given element. Accordingly, for each sample, only two solutions were prepared likewise in the one-point standard addition and two analytical runs were performed, yet four experimental points were generated for calibration according with the number of emission lines utilized. Based on the results obtained by analyzing urines from different subjects, the sample dilution fold was selected to adjust the analyte concentration below half of the calibration range (150 for K, 200 for Na, 50 for Ca and 25 for Mg), while the addition of standard was done roughly doubling natural concentration in the diluted sample. The evaluated instrumental limits of detection were: 0.009 ± 0.005 mg L-1 for K, 0.131 ± 0.011 mg L-1 for Na, 0.050 ± 0.014 mg L-1 for Ca and 0.059 ± 0.010 mg L-1 for Mg (five replicates in different days). Each analysis was performed in triplicate yielding percentage relative standard deviation ( 11 %. The percentage recoveries calculated taking the results obtained in acid-digested samples by external calibration as reference values were in the range: 83.3-102 % for K, 88.4-110 % for Na, 82.9-113 % for Ca and 85.8-108 % for Mg. The capability of the proposed MP-AES-MEC procedure for monitoring four elements in different clinical conditions was demonstrated by analyzing ten urines from diabetic patients and ten from non-diabetic control subjects; statistical differences between these two groups was found for Na and K. 2024 artículo científico 1870-249X https://www.redalyc.org/articulo.oa?id=47581848002 https://www.redalyc.org/journal/475/47581848002/ https://www.redalyc.org/journal/475/47581848002/html/ https://www.redalyc.org/journal/475/47581848002/47581848002.epub https://www.redalyc.org/journal/475/47581848002/movil 10.29356/jmcs.v68i1.1906 en http://www.redalyc.org/revista.oa?id=475 Journal of the Mexican Chemical Society application/pdf Sociedad Química de México Journal of the Mexican Chemical Society (México) Num.1 Vol.68