Effect of two cytokinins and a growth inhibitor on the in vitro tuberization of two genotypes of Solanum tuberosum L. cvs. Atlantic and Alpha
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| Format: | Artículo científico |
| Sprache: | en |
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Universidad Nacional de Costa Rica
2019
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| _version_ | 1876488769574535168 |
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| author | José Antonio García-García |
| author_facet | José Antonio García-García |
| contents | Effect of two cytokinins and a growth inhibitor on the in vitro tuberization of two genotypes of Solanum tuberosum L. cvs. Atlantic and Alpha José Antonio García-García José Bernal Azofeifa-Bolaños Frank Solano-Campos Rafael Orozco-Rodríguez Multidisciplinaria (Ciencias Naturales y Exactas) potato Microtuberization growth regulators Solanum tuberosum L This investigation was carried out to evaluate the effect of two cytokinins: 6-benzilaminopurine (BAP) (6.5 mg l-1) and kinetin (K) (2.5 mg l-1), as well as the growth inhibitor abscisic acid (ABA) (1.0 mg l-1) on the in vitro tuberization capacity of two potato varieties: Atlantic and Alpha. The basal culture medium MS (1962) was used as a control. The responses were different between varieties. In cv. Atlantic, the analysis of the number (NM), weight (WM) and diameter (DM) of microtubers indicated that the addition of growth regulators did not affect induction and development of microtubers. However, when BAP was used, a non-significant increment of 41 % was observed in the number of the microtubers compared to the control treatment, from 2.6 to 4.4. The addition of cytokinins and ABA to the medium did not have a significant impact on the development of microtubers. In cv. Alpha the cytokinins used without ABA increased the number of microtubers, which were larger and heavier than those of the control treatment. In this variety, ABA significantly reduced the values of the NM, WM and DM variables. The exogenous action of cytokinins in the culture medium is likely to have caused an endogenous hormonal imbalance in the Atlantic and Alpha genotypes which interfered with their innate microtuberization ability, a result that was even more evident for cv. Alpha, which showed the need to continue optimizing protocols of genotype-specific systems in potato tissue culture to increase yield and seed quality. 2019 artículo científico 2215-3470 https://www.redalyc.org/articulo.oa?id=475960592001 https://www.redalyc.org/journal/4759/475960592001/ https://www.redalyc.org/journal/4759/475960592001/html/ https://www.redalyc.org/journal/4759/475960592001/475960592001.epub https://www.redalyc.org/journal/4759/475960592001/movil 10.15359/ru.33-2.1 en http://www.redalyc.org/revista.oa?id=4759 Uniciencia application/pdf Universidad Nacional de Costa Rica Uniciencia (Costa Rica) Num.2 Vol.33 |
| format | Artículo científico |
| id | redalyc_475960592001 |
| institution | Redalyc |
| language | en |
| publishDate | 2019 |
| publisher | Universidad Nacional de Costa Rica |
| spellingShingle | Effect of two cytokinins and a growth inhibitor on the in vitro tuberization of two genotypes of Solanum tuberosum L. cvs. Atlantic and Alpha José Antonio García-García Multidisciplinaria (Ciencias Naturales y Exactas) potato Microtuberization growth regulators Solanum tuberosum L Effect of two cytokinins and a growth inhibitor on the in vitro tuberization of two genotypes of Solanum tuberosum L. cvs. Atlantic and Alpha José Antonio García-García José Bernal Azofeifa-Bolaños Frank Solano-Campos Rafael Orozco-Rodríguez Multidisciplinaria (Ciencias Naturales y Exactas) potato Microtuberization growth regulators Solanum tuberosum L This investigation was carried out to evaluate the effect of two cytokinins: 6-benzilaminopurine (BAP) (6.5 mg l-1) and kinetin (K) (2.5 mg l-1), as well as the growth inhibitor abscisic acid (ABA) (1.0 mg l-1) on the in vitro tuberization capacity of two potato varieties: Atlantic and Alpha. The basal culture medium MS (1962) was used as a control. The responses were different between varieties. In cv. Atlantic, the analysis of the number (NM), weight (WM) and diameter (DM) of microtubers indicated that the addition of growth regulators did not affect induction and development of microtubers. However, when BAP was used, a non-significant increment of 41 % was observed in the number of the microtubers compared to the control treatment, from 2.6 to 4.4. The addition of cytokinins and ABA to the medium did not have a significant impact on the development of microtubers. In cv. Alpha the cytokinins used without ABA increased the number of microtubers, which were larger and heavier than those of the control treatment. In this variety, ABA significantly reduced the values of the NM, WM and DM variables. The exogenous action of cytokinins in the culture medium is likely to have caused an endogenous hormonal imbalance in the Atlantic and Alpha genotypes which interfered with their innate microtuberization ability, a result that was even more evident for cv. Alpha, which showed the need to continue optimizing protocols of genotype-specific systems in potato tissue culture to increase yield and seed quality. 2019 artículo científico 2215-3470 https://www.redalyc.org/articulo.oa?id=475960592001 https://www.redalyc.org/journal/4759/475960592001/ https://www.redalyc.org/journal/4759/475960592001/html/ https://www.redalyc.org/journal/4759/475960592001/475960592001.epub https://www.redalyc.org/journal/4759/475960592001/movil 10.15359/ru.33-2.1 en http://www.redalyc.org/revista.oa?id=4759 Uniciencia application/pdf Universidad Nacional de Costa Rica Uniciencia (Costa Rica) Num.2 Vol.33 |
| title | Effect of two cytokinins and a growth inhibitor on the in vitro tuberization of two genotypes of Solanum tuberosum L. cvs. Atlantic and Alpha |
| topic | Multidisciplinaria (Ciencias Naturales y Exactas) potato Microtuberization growth regulators Solanum tuberosum L |
| url | https://www.redalyc.org/articulo.oa?id=475960592001 https://www.redalyc.org/journal/4759/475960592001/ https://www.redalyc.org/journal/4759/475960592001/html/ https://www.redalyc.org/journal/4759/475960592001/475960592001.epub https://www.redalyc.org/journal/4759/475960592001/movil |