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| Format: | Artículo científico |
| Language: | es |
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Universidad de Valparaíso
2014
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| Online Access: | https://www.redalyc.org/articulo.oa?id=47931255017 |
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Table of Contents:
- Diversidad de nematodos marinos de Chile continental y antártico: una evaluación morfológica y molecular Natalia Valderrama-Aravena Karla Pérez-Araneda Jorge Avaria-Llautureo Cristián E. Hernández Matthew Lee Antonio Brante Ciencias de la Tierra COI 18S nematode Key words Meiofauna Free-living nematodes are an important component of the marine benthos. In Chile there have been few studies on this group, and the majority has focused on the morphological aspect only. In this study, ribosomal 18S RNA and mitochondrial COI genes were used as genetic markers to complement morphological analyses to study the continental and Antarctic Chilean marine nematofauna. Different protocols for fixing, extracting and amplifying DNA were also tested. Not all the possible combinations produced good results. In fact, only the 18S gene showed consistently results. Phylogenetic analyses showed some discordance between classical taxonomy ( i.e., based on morphology) and molecular data. These results suggest that taxonomic classification using integrative approaches including morphological characteristics and molecular information is needed to study the diversity and evolution of this complex group. 2014 artículo científico 0717-3326 https://www.redalyc.org/articulo.oa?id=47931255017 es http://www.redalyc.org/revista.oa?id=479 Revista de Biología Marina y Oceanografía application/pdf Universidad de Valparaíso Revista de Biología Marina y Oceanografía (Chile) Num.1 Vol.49