Cytogenetic characterization of the silverside fish Odontesthes regia (Humboldt, 1833) (Teleostei: Atheriniformes: Atherinopsidae) from Iquique, Chile
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| Format: | Artículo científico |
| Language: | en |
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Universidad de Valparaíso
2006
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| _version_ | 1876440608419086336 |
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| author | Carlos Muñoz |
| author_facet | Carlos Muñoz |
| contents | Cytogenetic characterization of the silverside fish Odontesthes regia (Humboldt, 1833) (Teleostei: Atheriniformes: Atherinopsidae) from Iquique, Chile Carlos Muñoz Mauro Nirchio Julio E. Pérez Ernesto Ron Claudio Oliveira Irani A. Ferreira Ciencias de la Tierra NOR FISH bands Karyotype This paper describes the karyotype of Odontesthes regia by means of Giemsa staining, C-banding, to reveal the distribution of the constitutive heterochromatin, and by Ag-staining and fluorescent in situ hybridization (FISH), to locate ribosomal genes (rDNA). The chromosome diploid modal count in the species was 2n = 48. The karyotype is composed of one submetacentric pair (pair 1), 16 subtelocentric pairs (pairs 2 to 17), and 7 acrocentric pairs (pairs 18 to 24). With the exception of pair 1 it was not possible to classify the homologous chromosomes accurately because differences in chromosome size were too slight between adjacent pairs. The distribution of C-banded heterochromatin allowed for a more accurate matching of the majority of chromosomes of the subtelocentric series. Silver staining of metaphase spreads allowed for the identification of Nucleolus Organizer Regions (Ag-NOR) on pair 1. FISH experiments showed that 18S rDNA sequences were located, as expected, in the same chromosome pair identified as the Ag-NOR-bearing one. 2006 artículo científico 0717-3326 https://www.redalyc.org/articulo.oa?id=47941106 en http://www.redalyc.org/revista.oa?id=479 Revista de Biología Marina y Oceanografía application/pdf Universidad de Valparaíso Revista de Biología Marina y Oceanografía (Chile) Num.1 Vol.41 |
| format | Artículo científico |
| id | redalyc_47941106 |
| institution | Redalyc |
| language | en |
| publishDate | 2006 |
| publisher | Universidad de Valparaíso |
| spellingShingle | Cytogenetic characterization of the silverside fish Odontesthes regia (Humboldt, 1833) (Teleostei: Atheriniformes: Atherinopsidae) from Iquique, Chile Carlos Muñoz Ciencias de la Tierra NOR FISH bands Karyotype Cytogenetic characterization of the silverside fish Odontesthes regia (Humboldt, 1833) (Teleostei: Atheriniformes: Atherinopsidae) from Iquique, Chile Carlos Muñoz Mauro Nirchio Julio E. Pérez Ernesto Ron Claudio Oliveira Irani A. Ferreira Ciencias de la Tierra NOR FISH bands Karyotype This paper describes the karyotype of Odontesthes regia by means of Giemsa staining, C-banding, to reveal the distribution of the constitutive heterochromatin, and by Ag-staining and fluorescent in situ hybridization (FISH), to locate ribosomal genes (rDNA). The chromosome diploid modal count in the species was 2n = 48. The karyotype is composed of one submetacentric pair (pair 1), 16 subtelocentric pairs (pairs 2 to 17), and 7 acrocentric pairs (pairs 18 to 24). With the exception of pair 1 it was not possible to classify the homologous chromosomes accurately because differences in chromosome size were too slight between adjacent pairs. The distribution of C-banded heterochromatin allowed for a more accurate matching of the majority of chromosomes of the subtelocentric series. Silver staining of metaphase spreads allowed for the identification of Nucleolus Organizer Regions (Ag-NOR) on pair 1. FISH experiments showed that 18S rDNA sequences were located, as expected, in the same chromosome pair identified as the Ag-NOR-bearing one. 2006 artículo científico 0717-3326 https://www.redalyc.org/articulo.oa?id=47941106 en http://www.redalyc.org/revista.oa?id=479 Revista de Biología Marina y Oceanografía application/pdf Universidad de Valparaíso Revista de Biología Marina y Oceanografía (Chile) Num.1 Vol.41 |
| title | Cytogenetic characterization of the silverside fish Odontesthes regia (Humboldt, 1833) (Teleostei: Atheriniformes: Atherinopsidae) from Iquique, Chile |
| topic | Ciencias de la Tierra NOR FISH bands Karyotype |
| url | https://www.redalyc.org/articulo.oa?id=47941106 |