Isolation and characterization of culturable bacteria from tropical coastal waters
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Redalyc · Ciencias Marinas
In this study we isolated and characterized some culturable bacteria from tropical coastal waters of Peninsular Malaysia. We obtained between 0.23 and 1.85 × 103 cfu mL–1 in the Zobell 2216E medium, and cultured 0.04% to 0.12% of total bacterial counts. Different bacterial strains were then selected...
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| Pubblicato in: | Ciencias Marinas |
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| Natura: | Artículo científico |
| Lingua: | en |
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Universidad Autónoma de Baja California
2009
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| _version_ | 1878325299650756608 |
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| author | C-W Lee |
| author_facet | C-W Lee |
| collection | Ciencias Marinas |
| container_title | Ciencias Marinas |
| contents | Isolation and characterization of culturable bacteria from tropical coastal waters C-W Lee A Y-F Ng K Narayanan E U-H Sim C-C Ng Ciencias de la Tierra ZoBell 2216E 16S rDNA RFLP marine bacteria South China Sea Straits of Malacca In this study we isolated and characterized some culturable bacteria from tropical coastal waters of Peninsular Malaysia. We obtained between 0.23 and 1.85 × 103 cfu mL-1 in the Zobell 2216E medium, and cultured 0.04% to 0.12% of total bacterial counts. Different bacterial strains were then selected by 16S rDNA RFLP using four restriction enzymes (DdeI, HhaI, RsaI, and Sau3AI), of which HhaI gave the most RFLP patterns. A total of 54 unique strains were obtained and these were identified by their 16S rDNA gene sequence. These bacterial strains could be divided into five classes: 38 strains of -Proteobacteria (61.1%); 3 strains of -Proteobacteria (5.5%); 2 strains of the Cytophaga-Flavobacterium-Bacteroides group (3.7%); 3 strains of high GC, Gram-positive bacteria (5.5%); and 13 strains of low GC, Gram-positive bacteria (24.1%). These isolates have good potential for further biotechnological studies since about 56% of the isolates exhibited amylase activity, whereas 36% and 18% of the isolates had protease and lipase, respectively. Most (>70%) of the isolates also produced poly-B-hydroxybutyrate. 2009 artículo científico 0185-3880 https://www.redalyc.org/articulo.oa?id=48013186003 en http://www.redalyc.org/revista.oa?id=480 Ciencias Marinas application/pdf Universidad Autónoma de Baja California Ciencias Marinas (México) Num.2 Vol.35 |
| ctrlnum | oai:cienciasmarinas.com.mx:article/1525 10.7773/cm.v35i2.1525 |
| description | In this study we isolated and characterized some culturable bacteria from tropical coastal waters of Peninsular Malaysia. We obtained between 0.23 and 1.85 × 103 cfu mL–1 in the Zobell 2216E medium, and cultured 0.04% to 0.12% of total bacterial counts. Different bacterial strains were then selected by 16S rDNA RFLP using four restriction enzymes (DdeI, HhaI, RsaI, and Sau3AI), of which HhaI gave the most RFLP patterns. A total of 54 unique strains were obtained and these were identified by their 16S rDNA gene sequence. These bacterial strains could be divided into five classes: 38 strains of γ-Proteobacteria (61.1%); 3 strains of α-Proteobacteria (5.5%); 2 strains of the Cytophaga-Flavobacterium-Bacteroides group (3.7%); 3 strains of high GC, Gram-positive bacteria (5.5%); and 13 strains of low GC, Gram-positive bacteria (24.1%). These isolates have good potential for further biotechnological studies since about 56% of the isolates exhibited amylase activity, whereas 36% and 18% of the isolates had protease and lipase, respectively. Most (>70%) of the isolates also produced poly-ß-hydroxybutyrate.
En este estudio se aislaron y caracterizaron algunas bacterias cultivables de las aguas costeras tropicales de Malasia Peninsular. Se obtuvieron entre 0.23 y 1.85 × 103 ufc mL–1 en medio de cultivo Zobell 2216E, y se cultivaron 0.04% a 0.12% de los conteos totales de bacterias. Se seleccionaron diferentes cepas bacterianas mediante RFLP del gen 16S rDNA usando cuatro enzimas de restricción (DdeI, HhaI, RsaI y Sau3AI), de las cuales HhaI produjo más patrones de RFLP. Se obtuvieron un total de 54 cepas singulares, las cuales fueron identificadas por su secuencia 16S rDNA. Estas cepas bacterianas fueron divididas en cinco clases: 38 cepas de γ-proteobacterias (61.1%), 3 cepas de α-proteobacterias (5.5%), 2 cepas del grupo Cytophaga-Flavobacter-Bacteroides (3.7%), 3 cepas de bacterias Gram positivas con alto contenido de GC (5.5%) y 13 cepas de bacterias Gram positivas con bajo contenido de GC (24.1%). Estos aislados tienen un buen potencial para futuros estudios biotecnológicos ya que 56% de ellos presentaron actividad de la enzima amilasa, mientras que 36% y 18% presentaron actividad de las enzimas proteasa y lipasa, respectivamente. La mayoría (>70%) de los aislados produjeron poli-ß-hidroxibutirato. |
| format | Artículo científico |
| id | redalyc_48013186003 |
| institution | Redalyc Ciencias Marinas |
| language | en |
| publishDate | 2009 |
| publisher | Universidad Autónoma de Baja California |
| spelling | Isolation and characterization of culturable bacteria from tropical coastal waters Aislamiento y caracterización de bacterias cultivables de aguas costeras tropicales Lee, C-W Ng, A Y-F Narayanan, K Sim, E U-H Ng, C-C 16S rDNA RFLP marine bacteria South China Sea Straits of Malacca ZoBell 2216 16S rDNA RFLP bacterias marinas Estrecho de Malaca Mar de China Meridional ZoBell 2216E In this study we isolated and characterized some culturable bacteria from tropical coastal waters of Peninsular Malaysia. We obtained between 0.23 and 1.85 × 103 cfu mL–1 in the Zobell 2216E medium, and cultured 0.04% to 0.12% of total bacterial counts. Different bacterial strains were then selected by 16S rDNA RFLP using four restriction enzymes (DdeI, HhaI, RsaI, and Sau3AI), of which HhaI gave the most RFLP patterns. A total of 54 unique strains were obtained and these were identified by their 16S rDNA gene sequence. These bacterial strains could be divided into five classes: 38 strains of γ-Proteobacteria (61.1%); 3 strains of α-Proteobacteria (5.5%); 2 strains of the Cytophaga-Flavobacterium-Bacteroides group (3.7%); 3 strains of high GC, Gram-positive bacteria (5.5%); and 13 strains of low GC, Gram-positive bacteria (24.1%). These isolates have good potential for further biotechnological studies since about 56% of the isolates exhibited amylase activity, whereas 36% and 18% of the isolates had protease and lipase, respectively. Most (>70%) of the isolates also produced poly-ß-hydroxybutyrate. En este estudio se aislaron y caracterizaron algunas bacterias cultivables de las aguas costeras tropicales de Malasia Peninsular. Se obtuvieron entre 0.23 y 1.85 × 103 ufc mL–1 en medio de cultivo Zobell 2216E, y se cultivaron 0.04% a 0.12% de los conteos totales de bacterias. Se seleccionaron diferentes cepas bacterianas mediante RFLP del gen 16S rDNA usando cuatro enzimas de restricción (DdeI, HhaI, RsaI y Sau3AI), de las cuales HhaI produjo más patrones de RFLP. Se obtuvieron un total de 54 cepas singulares, las cuales fueron identificadas por su secuencia 16S rDNA. Estas cepas bacterianas fueron divididas en cinco clases: 38 cepas de γ-proteobacterias (61.1%), 3 cepas de α-proteobacterias (5.5%), 2 cepas del grupo Cytophaga-Flavobacter-Bacteroides (3.7%), 3 cepas de bacterias Gram positivas con alto contenido de GC (5.5%) y 13 cepas de bacterias Gram positivas con bajo contenido de GC (24.1%). Estos aislados tienen un buen potencial para futuros estudios biotecnológicos ya que 56% de ellos presentaron actividad de la enzima amilasa, mientras que 36% y 18% presentaron actividad de las enzimas proteasa y lipasa, respectivamente. La mayoría (>70%) de los aislados produjeron poli-ß-hidroxibutirato. Universidad Autónoma de Baja California, Instituto de Investigaciones Oceanológicas 2009-06-09 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article Artículo Arbitrado application/pdf https://cienciasmarinas.com.mx/index.php/cmarinas/article/view/1525 10.7773/cm.v35i2.1525 Ciencias Marinas; Vol. 35 No. 2 (2009); 153-167 Ciencias Marinas; Vol. 35 Núm. 2 (2009); 153-167 2395-9053 0185-3880 eng https://cienciasmarinas.com.mx/index.php/cmarinas/article/view/1525/1800 ['Redalyc'] redalyc_48013186003 Isolation and characterization of culturable bacteria from tropical coastal waters ['Isolation and characterization of culturable bacteria from tropical coastal waters', 'C-W Lee', 'Ciencias de la Tierra', 'ZoBell 2216E', '16S rDNA RFLP', 'marine bacteria', 'South China Sea', 'Straits of Malacca', 'Isolation and characterization of culturable bacteria from tropical coastal waters C-W Lee A Y-F Ng K Narayanan E U-H Sim C-C Ng Ciencias de la Tierra ZoBell 2216E 16S rDNA RFLP marine bacteria South China Sea Straits of Malacca In this study we isolated and characterized some culturable bacteria from tropical coastal waters of Peninsular Malaysia. We obtained between 0.23 and 1.85 × 103 cfu mL-1 in the Zobell 2216E medium, and cultured 0.04% to 0.12% of total bacterial counts. Different bacterial strains were then selected by 16S rDNA RFLP using four restriction enzymes (DdeI, HhaI, RsaI, and Sau3AI), of which HhaI gave the most RFLP patterns. A total of 54 unique strains were obtained and these were identified by their 16S rDNA gene sequence. These bacterial strains could be divided into five classes: 38 strains of -Proteobacteria (61.1%); 3 strains of -Proteobacteria (5.5%); 2 strains of the Cytophaga-Flavobacterium-Bacteroides group (3.7%); 3 strains of high GC, Gram-positive bacteria (5.5%); and 13 strains of low GC, Gram-positive bacteria (24.1%). These isolates have good potential for further biotechnological studies since about 56% of the isolates exhibited amylase activity, whereas 36% and 18% of the isolates had protease and lipase, respectively. Most (>70%) of the isolates also produced poly-B-hydroxybutyrate. 2009 artículo científico 0185-3880 https://www.redalyc.org/articulo.oa?id=48013186003 en http://www.redalyc.org/revista.oa?id=480 Ciencias Marinas application/pdf Universidad Autónoma de Baja California Ciencias Marinas (México) Num.2 Vol.35'] ['C-W Lee'] ['C-W Lee'] ['Artículo científico'] ['2009'] ['Universidad Autónoma de Baja California'] ['en'] ['Ciencias de la Tierra', 'ZoBell 2216E', '16S rDNA RFLP', 'marine bacteria', 'South China Sea', 'Straits of Malacca'] ['https://www.redalyc.org/articulo.oa?id=48013186003'] ['Isolation and characterization of culturable bacteria from tropical coastal waters C-W Lee A Y-F Ng K Narayanan E U-H Sim C-C Ng Ciencias de la Tierra ZoBell 2216E 16S rDNA RFLP marine bacteria South China Sea Straits of Malacca In this study we isolated and characterized some culturable bacteria from tropical coastal waters of Peninsular Malaysia. We obtained between 0.23 and 1.85 × 103 cfu mL-1 in the Zobell 2216E medium, and cultured 0.04% to 0.12% of total bacterial counts. Different bacterial strains were then selected by 16S rDNA RFLP using four restriction enzymes (DdeI, HhaI, RsaI, and Sau3AI), of which HhaI gave the most RFLP patterns. A total of 54 unique strains were obtained and these were identified by their 16S rDNA gene sequence. These bacterial strains could be divided into five classes: 38 strains of -Proteobacteria (61.1%); 3 strains of -Proteobacteria (5.5%); 2 strains of the Cytophaga-Flavobacterium-Bacteroides group (3.7%); 3 strains of high GC, Gram-positive bacteria (5.5%); and 13 strains of low GC, Gram-positive bacteria (24.1%). These isolates have good potential for further biotechnological studies since about 56% of the isolates exhibited amylase activity, whereas 36% and 18% of the isolates had protease and lipase, respectively. Most (>70%) of the isolates also produced poly-B-hydroxybutyrate. 2009 artículo científico 0185-3880 https://www.redalyc.org/articulo.oa?id=48013186003 en http://www.redalyc.org/revista.oa?id=480 Ciencias Marinas application/pdf Universidad Autónoma de Baja California Ciencias Marinas (México) Num.2 Vol.35'] |
| spellingShingle | Isolation and characterization of culturable bacteria from tropical coastal waters C-W Lee Ciencias de la Tierra ZoBell 2216E 16S rDNA RFLP marine bacteria South China Sea Straits of Malacca Isolation and characterization of culturable bacteria from tropical coastal waters C-W Lee A Y-F Ng K Narayanan E U-H Sim C-C Ng Ciencias de la Tierra ZoBell 2216E 16S rDNA RFLP marine bacteria South China Sea Straits of Malacca In this study we isolated and characterized some culturable bacteria from tropical coastal waters of Peninsular Malaysia. We obtained between 0.23 and 1.85 × 103 cfu mL-1 in the Zobell 2216E medium, and cultured 0.04% to 0.12% of total bacterial counts. Different bacterial strains were then selected by 16S rDNA RFLP using four restriction enzymes (DdeI, HhaI, RsaI, and Sau3AI), of which HhaI gave the most RFLP patterns. A total of 54 unique strains were obtained and these were identified by their 16S rDNA gene sequence. These bacterial strains could be divided into five classes: 38 strains of -Proteobacteria (61.1%); 3 strains of -Proteobacteria (5.5%); 2 strains of the Cytophaga-Flavobacterium-Bacteroides group (3.7%); 3 strains of high GC, Gram-positive bacteria (5.5%); and 13 strains of low GC, Gram-positive bacteria (24.1%). These isolates have good potential for further biotechnological studies since about 56% of the isolates exhibited amylase activity, whereas 36% and 18% of the isolates had protease and lipase, respectively. Most (>70%) of the isolates also produced poly-B-hydroxybutyrate. 2009 artículo científico 0185-3880 https://www.redalyc.org/articulo.oa?id=48013186003 en http://www.redalyc.org/revista.oa?id=480 Ciencias Marinas application/pdf Universidad Autónoma de Baja California Ciencias Marinas (México) Num.2 Vol.35 |
| title | Isolation and characterization of culturable bacteria from tropical coastal waters |
| title_alt | Aislamiento y caracterización de bacterias cultivables de aguas costeras tropicales |
| topic | Ciencias de la Tierra ZoBell 2216E 16S rDNA RFLP marine bacteria South China Sea Straits of Malacca |
| topic_facet | 16S rDNA RFLP marine bacteria South China Sea Straits of Malacca ZoBell 2216 bacterias marinas Estrecho de Malaca Mar de China Meridional ZoBell 2216E |
| url | https://www.redalyc.org/articulo.oa?id=48013186003 https://cienciasmarinas.com.mx/index.php/cmarinas/article/view/1525 |