Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Artículo científico |
| Sprache: | en |
| Veröffentlicht: |
Universidad Autónoma de Baja California
2011
|
| Schlagworte: | |
| Online-Zugang: | https://www.redalyc.org/articulo.oa?id=48020755010 |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
Inhaltsangabe:
- Isolation and prepurification of active compounds in venom from Pelagia noctiluca (Scyphozoa: Pelagiidae) from the Caribbean Sea J Sánchez-Rodríguez NL Lucio-Martínez Ciencias de la Tierra venom Isolation Caribbean Sea Pelagia nuctiluca Crude extract from Pelagia noctiluca was purified using liquid chromatography. The isolated fractions were tested in sea crabs (Ocypode quadrata) and neurotoxic activity was observed. The protein content of the crude extract, quantified by the Bradford method, was 0.25 mg mL¿1. Polyacrylamide gel electrophoresis was used to determine the apparent molecular weight of the different fractions of the crude extract, and several bands were identified: 34, 48, and between 90 and 200 kDa. Haemolytic activity in human blood type A+ was 100% with 1.1 ug mL¿1 and 50% with 0.98 ug mL¿1 of crude extract. The crude extract was observed under a microscope and three types of nematocysts were found: heterotrichous microbasic eurytele, heterotrichous isorhiza, and holotrichous isorhiza. 2011 artículo científico 0185-3880 https://www.redalyc.org/articulo.oa?id=48020755010 en http://www.redalyc.org/revista.oa?id=480 Ciencias Marinas application/pdf Universidad Autónoma de Baja California Ciencias Marinas (México) Num.3 Vol.37