Heavy metals and related biomarkers in Perna viridis (Bivalvia: Mytilidae) collected on the coast of Sucre State, Venezuela
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Redalyc · Ciencias Marinas
The green mussel Perna viridis has been used as a sentinel organism in marine and coastal ecosystem health assessments. In this study the degree of contamination at three sites on the coast of Sucre State (Venezuela) was analyzed using molecular, cellular, and immunological biomarkers in P. viridis....
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| Publicado en: | Ciencias Marinas |
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| Formato: | Artículo científico |
| Lenguaje: | en |
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Universidad Autónoma de Baja California
2012
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| _version_ | 1878325299962183680 |
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| author | E. Zapata-Vívenes |
| author_facet | E. Zapata-Vívenes |
| collection | Ciencias Marinas |
| container_title | Ciencias Marinas |
| contents | Heavy metals and related biomarkers in Perna viridis (Bivalvia: Mytilidae) collected on the coast of Sucre State, Venezuela E. Zapata-Vívenes L. Rojas de Astudillo G. Sánchez M. Barreto Ciencias de la Tierra hemocytes lysozymes heavy metals Perna viridis Antioxidant defenses The green mussel Perna viridis has been used as a sentinel organism in marine and coastal ecosystem health assessments. In this study the degree of contamination at three sites on the coast of Sucre State (Venezuela) was analyzed using molecular, cellular, and immunological biomarkers in P. viridis. Specimens were collected from Chacopata-Guayacán (CG, Araya Peninsula), Río Caribe (RC, Paria Peninsula), and San Antonio del Golfo (SAG, southern Gulf of Cariaco). We determined the concentration of heavy metals in the soft tissue of each individual, as well as the total number, viability, phagocytosis, and stability of the lysosomal membranes in hemocytes. The oxidative damage to lipids (TBARS), sulfhydryl group levels, total proteins, and lysozyme activity were quantified in the digestive gland. The results revealed high concentrations of Zn, Cr, and Cd in the organisms from CG. The total hemolymph cell count was lower in organisms from RC and CG. A slight increase in the number of phagocytes was observed in the organisms from RC and CG relative to those from SAG. The highest percentage of hemocytes with destabilized lysosomal membranes was recorded for the organisms from CG, followed by RC, indicating a weak association with the content of peroxidized lipids (TBARS); these values could be associated with the body loads of heavy metals. According to the results, the mussels from CG show slight signs of contamination by heavy metals, likely originated by human activities. The use of biological markers to estimate the effect of heavy metals on sentinel organisms is recommended. 2012 artículo científico 0185-3880 https://www.redalyc.org/articulo.oa?id=48024401004 en http://www.redalyc.org/revista.oa?id=480 Ciencias Marinas application/pdf Universidad Autónoma de Baja California Ciencias Marinas (México) Num.3 Vol.38 |
| ctrlnum | oai:cienciasmarinas.com.mx:article/2046 10.7773/cm.v38i3.2046 |
| description | The green mussel Perna viridis has been used as a sentinel organism in marine and coastal ecosystem health assessments. In this study the degree of contamination at three sites on the coast of Sucre State (Venezuela) was analyzed using molecular, cellular, and immunological biomarkers in P. viridis. Specimens were collected from Chacopata-Guayacán (CG, Araya Peninsula), Río Caribe (RC, Paria Peninsula), and San Antonio del Golfo (SAG, southern Gulf of Cariaco). We determined the concentration of heavy metals in the soft tissue of each individual, as well as the total number, viability, phagocytosis, and stability of the lysosomal membranes in hemocytes. The oxidative damage to lipids (TBARS), sulfhydryl group levels, total proteins, and lysozyme activity were quantified in the digestive gland. The results revealed high concentrations of Zn, Cr, and Cd in the organisms from CG. The total hemolymph cell count was lower in organisms from RC and CG. A slight increase in the number of phagocytes was observed in the organisms from RC and CG relative to those from SAG. The highest percentage of hemocytes with destabilized lysosomal membranes was recorded for the organisms from CG, followed by RC, indicating a weak association with the content of peroxidized lipids (TBARS); these values could be associated with the body loads of heavy metals. According to the results, the mussels from CG show slight signs of contamination by heavy metals, likely originated by human activities. The use of biological markers to estimate the effect of heavy metals on sentinel organisms is recommended.
El mejillón verde Perna viridis ha sido usado como organismo biosensor de la salud de ecosistemas marino-costeros. Por tal motivo se procedió a evaluar el grado de contaminación de tres localidades costeras del estado Sucre (Venezuela) determinando biomarcadores a nivel molecular, celular e inmunológico en P. viridis. Los ejemplares fueron recolectados en las localidades de Chacopata-Guayacán (CG, península de Araya), Río Caribe (RC, península de Paria) y San Antonio del Golfo (SAG, región sur del golfo de Cariaco). Se determinó la carga de metales pesados en el tejido blando de cada individuo. Además, se valoró el número total, la viabilidad, la fagocitosis y la estabilidad de las membranas lisosomales en los hemocitos. El daño oxidativo de lípidos (TBARS), los niveles de grupos sulfhídrilos, las proteínas totales y la actividad de la lisozima fueron cuantificados en la glándula digestiva. Los resultados evidencian altas concentraciones de Zn, Cr y Cd en organismos provenientes de CG. El número de células totales en hemolinfa fue menor en los organismos de RC y CG. En estas últimas zonas los mejillones presentaron un ligero incremento en número de fagocitos con respecto a SAG. El mayor porcentaje de hemocitos con membranas lisosomales desestabilizadas fue encontrado en los organismos de CG, seguido de RC, evidenciándose una débil asociación con el contenido de lípidos peroxidados (TBARS); estos valores podrían tener correspondencia con la acumulación corporal de metales pesados. Los resultados revelan que los mejillones de CG muestran ligeros indicios de contaminación por metales pesados, originados presumiblemente por actividades antropogénicas. Esta investigación sugiere la utilidad de marcadores biológicos destinados a estimar el impacto de metales pesados en organismos centinelas. |
| format | Artículo científico |
| id | redalyc_48024401004 |
| institution | Redalyc Ciencias Marinas |
| language | en |
| publishDate | 2012 |
| publisher | Universidad Autónoma de Baja California |
| spelling | Heavy metals and related biomarkers in Perna viridis (Bivalvia: Mytilidae) collected on the coast of Sucre State, Venezuela Metales pesados y biomarcadores relacionados en Perna viridis (Bivalvia: Mytilidae) recolectado en las costas del estado Sucre, Venezuela Zapata-Vívenes, E Rojas de Astudillo, L Sánchez, G Barreto, M The green mussel Perna viridis has been used as a sentinel organism in marine and coastal ecosystem health assessments. In this study the degree of contamination at three sites on the coast of Sucre State (Venezuela) was analyzed using molecular, cellular, and immunological biomarkers in P. viridis. Specimens were collected from Chacopata-Guayacán (CG, Araya Peninsula), Río Caribe (RC, Paria Peninsula), and San Antonio del Golfo (SAG, southern Gulf of Cariaco). We determined the concentration of heavy metals in the soft tissue of each individual, as well as the total number, viability, phagocytosis, and stability of the lysosomal membranes in hemocytes. The oxidative damage to lipids (TBARS), sulfhydryl group levels, total proteins, and lysozyme activity were quantified in the digestive gland. The results revealed high concentrations of Zn, Cr, and Cd in the organisms from CG. The total hemolymph cell count was lower in organisms from RC and CG. A slight increase in the number of phagocytes was observed in the organisms from RC and CG relative to those from SAG. The highest percentage of hemocytes with destabilized lysosomal membranes was recorded for the organisms from CG, followed by RC, indicating a weak association with the content of peroxidized lipids (TBARS); these values could be associated with the body loads of heavy metals. According to the results, the mussels from CG show slight signs of contamination by heavy metals, likely originated by human activities. The use of biological markers to estimate the effect of heavy metals on sentinel organisms is recommended. El mejillón verde Perna viridis ha sido usado como organismo biosensor de la salud de ecosistemas marino-costeros. Por tal motivo se procedió a evaluar el grado de contaminación de tres localidades costeras del estado Sucre (Venezuela) determinando biomarcadores a nivel molecular, celular e inmunológico en P. viridis. Los ejemplares fueron recolectados en las localidades de Chacopata-Guayacán (CG, península de Araya), Río Caribe (RC, península de Paria) y San Antonio del Golfo (SAG, región sur del golfo de Cariaco). Se determinó la carga de metales pesados en el tejido blando de cada individuo. Además, se valoró el número total, la viabilidad, la fagocitosis y la estabilidad de las membranas lisosomales en los hemocitos. El daño oxidativo de lípidos (TBARS), los niveles de grupos sulfhídrilos, las proteínas totales y la actividad de la lisozima fueron cuantificados en la glándula digestiva. Los resultados evidencian altas concentraciones de Zn, Cr y Cd en organismos provenientes de CG. El número de células totales en hemolinfa fue menor en los organismos de RC y CG. En estas últimas zonas los mejillones presentaron un ligero incremento en número de fagocitos con respecto a SAG. El mayor porcentaje de hemocitos con membranas lisosomales desestabilizadas fue encontrado en los organismos de CG, seguido de RC, evidenciándose una débil asociación con el contenido de lípidos peroxidados (TBARS); estos valores podrían tener correspondencia con la acumulación corporal de metales pesados. Los resultados revelan que los mejillones de CG muestran ligeros indicios de contaminación por metales pesados, originados presumiblemente por actividades antropogénicas. Esta investigación sugiere la utilidad de marcadores biológicos destinados a estimar el impacto de metales pesados en organismos centinelas. Universidad Autónoma de Baja California, Instituto de Investigaciones Oceanológicas 2012-06-06 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article Artículo Arbitrado application/pdf https://cienciasmarinas.com.mx/index.php/cmarinas/article/view/2046 10.7773/cm.v38i3.2046 Ciencias Marinas; Vol. 38 No. 3 (2012); 517-528 Ciencias Marinas; Vol. 38 Núm. 3 (2012); 517-528 2395-9053 0185-3880 eng https://cienciasmarinas.com.mx/index.php/cmarinas/article/view/2046/1413 ['Redalyc'] redalyc_48024401004 Heavy metals and related biomarkers in Perna viridis (Bivalvia: Mytilidae) collected on the coast of Sucre State, Venezuela ['Heavy metals and related biomarkers in Perna viridis (Bivalvia: Mytilidae) collected on the coast of Sucre State, Venezuela', 'E. Zapata-Vívenes', 'Ciencias de la Tierra', 'hemocytes', 'lysozymes', 'heavy metals', 'Perna viridis', 'Antioxidant defenses', 'Heavy metals and related biomarkers in Perna viridis (Bivalvia: Mytilidae) collected on the coast of Sucre State, Venezuela E. Zapata-Vívenes L. Rojas de Astudillo G. Sánchez M. Barreto Ciencias de la Tierra hemocytes lysozymes heavy metals Perna viridis Antioxidant defenses The green mussel Perna viridis has been used as a sentinel organism in marine and coastal ecosystem health assessments. In this study the degree of contamination at three sites on the coast of Sucre State (Venezuela) was analyzed using molecular, cellular, and immunological biomarkers in P. viridis. Specimens were collected from Chacopata-Guayacán (CG, Araya Peninsula), Río Caribe (RC, Paria Peninsula), and San Antonio del Golfo (SAG, southern Gulf of Cariaco). We determined the concentration of heavy metals in the soft tissue of each individual, as well as the total number, viability, phagocytosis, and stability of the lysosomal membranes in hemocytes. The oxidative damage to lipids (TBARS), sulfhydryl group levels, total proteins, and lysozyme activity were quantified in the digestive gland. The results revealed high concentrations of Zn, Cr, and Cd in the organisms from CG. The total hemolymph cell count was lower in organisms from RC and CG. A slight increase in the number of phagocytes was observed in the organisms from RC and CG relative to those from SAG. The highest percentage of hemocytes with destabilized lysosomal membranes was recorded for the organisms from CG, followed by RC, indicating a weak association with the content of peroxidized lipids (TBARS); these values could be associated with the body loads of heavy metals. According to the results, the mussels from CG show slight signs of contamination by heavy metals, likely originated by human activities. The use of biological markers to estimate the effect of heavy metals on sentinel organisms is recommended. 2012 artículo científico 0185-3880 https://www.redalyc.org/articulo.oa?id=48024401004 en http://www.redalyc.org/revista.oa?id=480 Ciencias Marinas application/pdf Universidad Autónoma de Baja California Ciencias Marinas (México) Num.3 Vol.38'] ['E. Zapata-Vívenes'] ['E. Zapata-Vívenes'] ['Artículo científico'] ['2012'] ['Universidad Autónoma de Baja California'] ['en'] ['Ciencias de la Tierra', 'hemocytes', 'lysozymes', 'heavy metals', 'Perna viridis', 'Antioxidant defenses'] ['https://www.redalyc.org/articulo.oa?id=48024401004'] ['Heavy metals and related biomarkers in Perna viridis (Bivalvia: Mytilidae) collected on the coast of Sucre State, Venezuela E. Zapata-Vívenes L. Rojas de Astudillo G. Sánchez M. Barreto Ciencias de la Tierra hemocytes lysozymes heavy metals Perna viridis Antioxidant defenses The green mussel Perna viridis has been used as a sentinel organism in marine and coastal ecosystem health assessments. In this study the degree of contamination at three sites on the coast of Sucre State (Venezuela) was analyzed using molecular, cellular, and immunological biomarkers in P. viridis. Specimens were collected from Chacopata-Guayacán (CG, Araya Peninsula), Río Caribe (RC, Paria Peninsula), and San Antonio del Golfo (SAG, southern Gulf of Cariaco). We determined the concentration of heavy metals in the soft tissue of each individual, as well as the total number, viability, phagocytosis, and stability of the lysosomal membranes in hemocytes. The oxidative damage to lipids (TBARS), sulfhydryl group levels, total proteins, and lysozyme activity were quantified in the digestive gland. The results revealed high concentrations of Zn, Cr, and Cd in the organisms from CG. The total hemolymph cell count was lower in organisms from RC and CG. A slight increase in the number of phagocytes was observed in the organisms from RC and CG relative to those from SAG. The highest percentage of hemocytes with destabilized lysosomal membranes was recorded for the organisms from CG, followed by RC, indicating a weak association with the content of peroxidized lipids (TBARS); these values could be associated with the body loads of heavy metals. According to the results, the mussels from CG show slight signs of contamination by heavy metals, likely originated by human activities. The use of biological markers to estimate the effect of heavy metals on sentinel organisms is recommended. 2012 artículo científico 0185-3880 https://www.redalyc.org/articulo.oa?id=48024401004 en http://www.redalyc.org/revista.oa?id=480 Ciencias Marinas application/pdf Universidad Autónoma de Baja California Ciencias Marinas (México) Num.3 Vol.38'] |
| spellingShingle | Heavy metals and related biomarkers in Perna viridis (Bivalvia: Mytilidae) collected on the coast of Sucre State, Venezuela E. Zapata-Vívenes Ciencias de la Tierra hemocytes lysozymes heavy metals Perna viridis Antioxidant defenses Heavy metals and related biomarkers in Perna viridis (Bivalvia: Mytilidae) collected on the coast of Sucre State, Venezuela E. Zapata-Vívenes L. Rojas de Astudillo G. Sánchez M. Barreto Ciencias de la Tierra hemocytes lysozymes heavy metals Perna viridis Antioxidant defenses The green mussel Perna viridis has been used as a sentinel organism in marine and coastal ecosystem health assessments. In this study the degree of contamination at three sites on the coast of Sucre State (Venezuela) was analyzed using molecular, cellular, and immunological biomarkers in P. viridis. Specimens were collected from Chacopata-Guayacán (CG, Araya Peninsula), Río Caribe (RC, Paria Peninsula), and San Antonio del Golfo (SAG, southern Gulf of Cariaco). We determined the concentration of heavy metals in the soft tissue of each individual, as well as the total number, viability, phagocytosis, and stability of the lysosomal membranes in hemocytes. The oxidative damage to lipids (TBARS), sulfhydryl group levels, total proteins, and lysozyme activity were quantified in the digestive gland. The results revealed high concentrations of Zn, Cr, and Cd in the organisms from CG. The total hemolymph cell count was lower in organisms from RC and CG. A slight increase in the number of phagocytes was observed in the organisms from RC and CG relative to those from SAG. The highest percentage of hemocytes with destabilized lysosomal membranes was recorded for the organisms from CG, followed by RC, indicating a weak association with the content of peroxidized lipids (TBARS); these values could be associated with the body loads of heavy metals. According to the results, the mussels from CG show slight signs of contamination by heavy metals, likely originated by human activities. The use of biological markers to estimate the effect of heavy metals on sentinel organisms is recommended. 2012 artículo científico 0185-3880 https://www.redalyc.org/articulo.oa?id=48024401004 en http://www.redalyc.org/revista.oa?id=480 Ciencias Marinas application/pdf Universidad Autónoma de Baja California Ciencias Marinas (México) Num.3 Vol.38 |
| title | Heavy metals and related biomarkers in Perna viridis (Bivalvia: Mytilidae) collected on the coast of Sucre State, Venezuela |
| title_alt | Metales pesados y biomarcadores relacionados en Perna viridis (Bivalvia: Mytilidae) recolectado en las costas del estado Sucre, Venezuela |
| topic | Ciencias de la Tierra hemocytes lysozymes heavy metals Perna viridis Antioxidant defenses |
| url | https://www.redalyc.org/articulo.oa?id=48024401004 https://cienciasmarinas.com.mx/index.php/cmarinas/article/view/2046 |