Evaluation of the Gulf of California as a potential source of bioactive marine actinobacteria
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Redalyc · Ciencias Marinas
Actinobacteria produce many bioactive compounds currently used as antibiotics and anticancer drugs. The objective of this project was to evaluate the Gulf of California as a novel source of bioactive actinobacterial strains. A total of 235 actinobacterial strains were isolated from marine sediment c...
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| Publié dans: | Ciencias Marinas |
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| Format: | Artículo científico |
| Langue: | en |
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Universidad Autónoma de Baja California
2012
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| _version_ | 1878325299990495232 |
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| author | M Torres-Beltrán |
| author_facet | M Torres-Beltrán |
| collection | Ciencias Marinas |
| container_title | Ciencias Marinas |
| contents | Evaluation of the Gulf of California as a potential source of bioactive marine actinobacteria M Torres-Beltrán F Cardoso-Martínez N Millán-Aguiñaga A Becerril-Espinosa IE Soria-Mercado Ciencias de la Tierra cytotoxic antibiotic Salinispora Gulf of California Marine actinobacteria Actinobacteria produce many bioactive compounds currently used as antibiotics and anticancer drugs. The objective of this project was to evaluate the Gulf of California as a novel source of bioactive actinobacterial strains. A total of 235 actinobacterial strains were isolated from marine sediment collected in Concepción and los Ángeles bays (Mexico). Based on their morphology, seawater requirements, and 16S rRNA sequencing, actinobacterial strains were classified as Streptomyces, Micromonospora, and Salinispora. Sixty-nine organic and aqueous extracts were obtained using liquid-liquid extraction with ethyl acetate; 17 showed cytotoxic activity against breast cancer cells (MCF7) and cervical cancer cells (HeLa). The highest activity values observed, expressed as survival percentage, were 20–25% against MCF7 cells (strains S-365, S-355, and S-361) and 24–25% against HeLa cells (strains S-165, S-361, and S-353). Only three aqueous extracts showed antibiotic activity towards methicillin-resistant Staphylococcus aureus, with activity values of 3% and 6% for strains S-370 and S-369, respectively. Molecular weights found by liquid chromatography-mass spectrometry analysis are reported for Micromonospora species isolated from soil, but no species-specific secondary metabolite evidence was observed for Salinispora isolates. The biological activity observed in this work offers opportunities for further chemical studies to define the compounds responsible for this activity in order to contribute to the discovery of new drugs and to acknowledge the Gulf of California as a reservoir of marine bioactive actinobacteria strains that are important for human health. 2012 artículo científico 0185-3880 https://www.redalyc.org/articulo.oa?id=48025112003 en http://www.redalyc.org/revista.oa?id=480 Ciencias Marinas application/pdf Universidad Autónoma de Baja California Ciencias Marinas (México) Num.4 Vol.38 |
| ctrlnum | oai:cienciasmarinas.com.mx:article/2131 10.7773/cm.v38i4.2131 |
| description | Actinobacteria produce many bioactive compounds currently used as antibiotics and anticancer drugs. The objective of this project was to evaluate the Gulf of California as a novel source of bioactive actinobacterial strains. A total of 235 actinobacterial strains were isolated from marine sediment collected in Concepción and los Ángeles bays (Mexico). Based on their morphology, seawater requirements, and 16S rRNA sequencing, actinobacterial strains were classified as Streptomyces, Micromonospora, and Salinispora. Sixty-nine organic and aqueous extracts were obtained using liquid-liquid extraction with ethyl acetate; 17 showed cytotoxic activity against breast cancer cells (MCF7) and cervical cancer cells (HeLa). The highest activity values observed, expressed as survival percentage, were 20–25% against MCF7 cells (strains S-365, S-355, and S-361) and 24–25% against HeLa cells (strains S-165, S-361, and S-353). Only three aqueous extracts showed antibiotic activity towards methicillin-resistant Staphylococcus aureus, with activity values of 3% and 6% for strains S-370 and S-369, respectively. Molecular weights found by liquid chromatography-mass spectrometry analysis are reported for Micromonospora species isolated from soil, but no species specific secondary metabolite evidence was observed for Salinispora isolates. The biological activity observed in this work offers opportunities for further chemical studies to define the compounds responsible for this activity in order to contribute to the discovery of new drugs and to acknowledge the Gulf of California as a reservoir of marine bioactive actinobacteria strains that are important for human health.
Las actinobacterias son productoras de una gran variedad de compuestos utilizados actualmente como antibióticos y anticancerígenos. En este trabajo se evaluó el potencial del golfo de California como fuente de cepas de actinobacterias bioactivas. En total, se aislaron 235 cepas de actinobacterias de los sedimentos de bahía Concepción y bahía de los Ángeles, (México). Con base en su morfología,requerimiento de agua de mar para su crecimiento y secuenciación del gen 16S del ARNr, las cepas se clasificaron como Streptomyces, Micromonospora y Salinispora. Mediante la técnica de extracción líquido-líquido con acetato de etilo como disolvente, se obtuvieron 69 extractos orgánicos y acuosos; 17 mostraron actividad citotóxica contra las líneas celulares de cáncer de mama (MCF7) y cérvix (HeLa). Los valores máximos de actividad, expresados como porcentaje de supervivencia, fueron de 20–25% contra MCF7 (cepas S-365, S-355 y S-361) y de 24–25% contra HeLa (cepas S-165, S-361 y S-353). Tres extractos en su fracción acuosa mostraron actividad antibiótica contra Staphylococcus aureus, que es resistente a la meticilina, con valores de actividad de 3% y 6% (cepas S-370 y S-369, respectivamente). Con base en el análisis de cromatografía líquida con detector de masas, se encontraron pesos moleculares de compuestos previamente documentadospara especies terrestres del genero Micromonospora; sin embargo, no se identificaron los pesos moleculares de metabolitos específicospreviamente documentados para las cepas del género Salinispora. La bioactividad observada en este trabajo ofrece la oportunidad de un estudio químico posterior para definir los metabolitos responsables de la actividad a fin de contribuir con el descubrimiento de nuevos fármacos y ubicar al golfo de California como una fuente potencial de cepas de actinobacterias productoras de compuestos bioactivos de importancia para la salud humana. |
| format | Artículo científico |
| id | redalyc_48025112003 |
| institution | Redalyc Ciencias Marinas |
| language | en |
| publishDate | 2012 |
| publisher | Universidad Autónoma de Baja California |
| spelling | Evaluation of the Gulf of California as a potential source of bioactive marine actinobacteria Evaluación del golfo de California como una fuente potencial de actinobacterias marinas bioactivas Torres-Beltrán, M Cardoso-Martínez, F Millán-Aguiñaga, N Becerril-Espinosa, A Soria-Mercado, IE Actinobacteria produce many bioactive compounds currently used as antibiotics and anticancer drugs. The objective of this project was to evaluate the Gulf of California as a novel source of bioactive actinobacterial strains. A total of 235 actinobacterial strains were isolated from marine sediment collected in Concepción and los Ángeles bays (Mexico). Based on their morphology, seawater requirements, and 16S rRNA sequencing, actinobacterial strains were classified as Streptomyces, Micromonospora, and Salinispora. Sixty-nine organic and aqueous extracts were obtained using liquid-liquid extraction with ethyl acetate; 17 showed cytotoxic activity against breast cancer cells (MCF7) and cervical cancer cells (HeLa). The highest activity values observed, expressed as survival percentage, were 20–25% against MCF7 cells (strains S-365, S-355, and S-361) and 24–25% against HeLa cells (strains S-165, S-361, and S-353). Only three aqueous extracts showed antibiotic activity towards methicillin-resistant Staphylococcus aureus, with activity values of 3% and 6% for strains S-370 and S-369, respectively. Molecular weights found by liquid chromatography-mass spectrometry analysis are reported for Micromonospora species isolated from soil, but no species specific secondary metabolite evidence was observed for Salinispora isolates. The biological activity observed in this work offers opportunities for further chemical studies to define the compounds responsible for this activity in order to contribute to the discovery of new drugs and to acknowledge the Gulf of California as a reservoir of marine bioactive actinobacteria strains that are important for human health. Las actinobacterias son productoras de una gran variedad de compuestos utilizados actualmente como antibióticos y anticancerígenos. En este trabajo se evaluó el potencial del golfo de California como fuente de cepas de actinobacterias bioactivas. En total, se aislaron 235 cepas de actinobacterias de los sedimentos de bahía Concepción y bahía de los Ángeles, (México). Con base en su morfología,requerimiento de agua de mar para su crecimiento y secuenciación del gen 16S del ARNr, las cepas se clasificaron como Streptomyces, Micromonospora y Salinispora. Mediante la técnica de extracción líquido-líquido con acetato de etilo como disolvente, se obtuvieron 69 extractos orgánicos y acuosos; 17 mostraron actividad citotóxica contra las líneas celulares de cáncer de mama (MCF7) y cérvix (HeLa). Los valores máximos de actividad, expresados como porcentaje de supervivencia, fueron de 20–25% contra MCF7 (cepas S-365, S-355 y S-361) y de 24–25% contra HeLa (cepas S-165, S-361 y S-353). Tres extractos en su fracción acuosa mostraron actividad antibiótica contra Staphylococcus aureus, que es resistente a la meticilina, con valores de actividad de 3% y 6% (cepas S-370 y S-369, respectivamente). Con base en el análisis de cromatografía líquida con detector de masas, se encontraron pesos moleculares de compuestos previamente documentadospara especies terrestres del genero Micromonospora; sin embargo, no se identificaron los pesos moleculares de metabolitos específicospreviamente documentados para las cepas del género Salinispora. La bioactividad observada en este trabajo ofrece la oportunidad de un estudio químico posterior para definir los metabolitos responsables de la actividad a fin de contribuir con el descubrimiento de nuevos fármacos y ubicar al golfo de California como una fuente potencial de cepas de actinobacterias productoras de compuestos bioactivos de importancia para la salud humana. Universidad Autónoma de Baja California, Instituto de Investigaciones Oceanológicas 2012-12-01 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article Artículo Arbitrado application/pdf https://cienciasmarinas.com.mx/index.php/cmarinas/article/view/2131 10.7773/cm.v38i4.2131 Ciencias Marinas; Vol. 38 No. 4 (2012); 609-624 Ciencias Marinas; Vol. 38 Núm. 4 (2012); 609-624 2395-9053 0185-3880 eng https://cienciasmarinas.com.mx/index.php/cmarinas/article/view/2131/1418 ['Redalyc'] redalyc_48025112003 Evaluation of the Gulf of California as a potential source of bioactive marine actinobacteria ['Evaluation of the Gulf of California as a potential source of bioactive marine actinobacteria', 'M Torres-Beltrán', 'Ciencias de la Tierra', 'cytotoxic', 'antibiotic', 'Salinispora', 'Gulf of California', 'Marine actinobacteria', 'Evaluation of the Gulf of California as a potential source of bioactive marine actinobacteria M Torres-Beltrán F Cardoso-Martínez N Millán-Aguiñaga A Becerril-Espinosa IE Soria-Mercado Ciencias de la Tierra cytotoxic antibiotic Salinispora Gulf of California Marine actinobacteria Actinobacteria produce many bioactive compounds currently used as antibiotics and anticancer drugs. The objective of this project was to evaluate the Gulf of California as a novel source of bioactive actinobacterial strains. A total of 235 actinobacterial strains were isolated from marine sediment collected in Concepción and los Ángeles bays (Mexico). Based on their morphology, seawater requirements, and 16S rRNA sequencing, actinobacterial strains were classified as Streptomyces, Micromonospora, and Salinispora. Sixty-nine organic and aqueous extracts were obtained using liquid-liquid extraction with ethyl acetate; 17 showed cytotoxic activity against breast cancer cells (MCF7) and cervical cancer cells (HeLa). The highest activity values observed, expressed as survival percentage, were 20–25% against MCF7 cells (strains S-365, S-355, and S-361) and 24–25% against HeLa cells (strains S-165, S-361, and S-353). Only three aqueous extracts showed antibiotic activity towards methicillin-resistant Staphylococcus aureus, with activity values of 3% and 6% for strains S-370 and S-369, respectively. Molecular weights found by liquid chromatography-mass spectrometry analysis are reported for Micromonospora species isolated from soil, but no species-specific secondary metabolite evidence was observed for Salinispora isolates. The biological activity observed in this work offers opportunities for further chemical studies to define the compounds responsible for this activity in order to contribute to the discovery of new drugs and to acknowledge the Gulf of California as a reservoir of marine bioactive actinobacteria strains that are important for human health. 2012 artículo científico 0185-3880 https://www.redalyc.org/articulo.oa?id=48025112003 en http://www.redalyc.org/revista.oa?id=480 Ciencias Marinas application/pdf Universidad Autónoma de Baja California Ciencias Marinas (México) Num.4 Vol.38'] ['M Torres-Beltrán'] ['M Torres-Beltrán'] ['Artículo científico'] ['2012'] ['Universidad Autónoma de Baja California'] ['en'] ['Ciencias de la Tierra', 'cytotoxic', 'antibiotic', 'Salinispora', 'Gulf of California', 'Marine actinobacteria'] ['https://www.redalyc.org/articulo.oa?id=48025112003'] ['Evaluation of the Gulf of California as a potential source of bioactive marine actinobacteria M Torres-Beltrán F Cardoso-Martínez N Millán-Aguiñaga A Becerril-Espinosa IE Soria-Mercado Ciencias de la Tierra cytotoxic antibiotic Salinispora Gulf of California Marine actinobacteria Actinobacteria produce many bioactive compounds currently used as antibiotics and anticancer drugs. The objective of this project was to evaluate the Gulf of California as a novel source of bioactive actinobacterial strains. A total of 235 actinobacterial strains were isolated from marine sediment collected in Concepción and los Ángeles bays (Mexico). Based on their morphology, seawater requirements, and 16S rRNA sequencing, actinobacterial strains were classified as Streptomyces, Micromonospora, and Salinispora. Sixty-nine organic and aqueous extracts were obtained using liquid-liquid extraction with ethyl acetate; 17 showed cytotoxic activity against breast cancer cells (MCF7) and cervical cancer cells (HeLa). The highest activity values observed, expressed as survival percentage, were 20–25% against MCF7 cells (strains S-365, S-355, and S-361) and 24–25% against HeLa cells (strains S-165, S-361, and S-353). Only three aqueous extracts showed antibiotic activity towards methicillin-resistant Staphylococcus aureus, with activity values of 3% and 6% for strains S-370 and S-369, respectively. Molecular weights found by liquid chromatography-mass spectrometry analysis are reported for Micromonospora species isolated from soil, but no species-specific secondary metabolite evidence was observed for Salinispora isolates. The biological activity observed in this work offers opportunities for further chemical studies to define the compounds responsible for this activity in order to contribute to the discovery of new drugs and to acknowledge the Gulf of California as a reservoir of marine bioactive actinobacteria strains that are important for human health. 2012 artículo científico 0185-3880 https://www.redalyc.org/articulo.oa?id=48025112003 en http://www.redalyc.org/revista.oa?id=480 Ciencias Marinas application/pdf Universidad Autónoma de Baja California Ciencias Marinas (México) Num.4 Vol.38'] |
| spellingShingle | Evaluation of the Gulf of California as a potential source of bioactive marine actinobacteria M Torres-Beltrán Ciencias de la Tierra cytotoxic antibiotic Salinispora Gulf of California Marine actinobacteria Evaluation of the Gulf of California as a potential source of bioactive marine actinobacteria M Torres-Beltrán F Cardoso-Martínez N Millán-Aguiñaga A Becerril-Espinosa IE Soria-Mercado Ciencias de la Tierra cytotoxic antibiotic Salinispora Gulf of California Marine actinobacteria Actinobacteria produce many bioactive compounds currently used as antibiotics and anticancer drugs. The objective of this project was to evaluate the Gulf of California as a novel source of bioactive actinobacterial strains. A total of 235 actinobacterial strains were isolated from marine sediment collected in Concepción and los Ángeles bays (Mexico). Based on their morphology, seawater requirements, and 16S rRNA sequencing, actinobacterial strains were classified as Streptomyces, Micromonospora, and Salinispora. Sixty-nine organic and aqueous extracts were obtained using liquid-liquid extraction with ethyl acetate; 17 showed cytotoxic activity against breast cancer cells (MCF7) and cervical cancer cells (HeLa). The highest activity values observed, expressed as survival percentage, were 20–25% against MCF7 cells (strains S-365, S-355, and S-361) and 24–25% against HeLa cells (strains S-165, S-361, and S-353). Only three aqueous extracts showed antibiotic activity towards methicillin-resistant Staphylococcus aureus, with activity values of 3% and 6% for strains S-370 and S-369, respectively. Molecular weights found by liquid chromatography-mass spectrometry analysis are reported for Micromonospora species isolated from soil, but no species-specific secondary metabolite evidence was observed for Salinispora isolates. The biological activity observed in this work offers opportunities for further chemical studies to define the compounds responsible for this activity in order to contribute to the discovery of new drugs and to acknowledge the Gulf of California as a reservoir of marine bioactive actinobacteria strains that are important for human health. 2012 artículo científico 0185-3880 https://www.redalyc.org/articulo.oa?id=48025112003 en http://www.redalyc.org/revista.oa?id=480 Ciencias Marinas application/pdf Universidad Autónoma de Baja California Ciencias Marinas (México) Num.4 Vol.38 |
| title | Evaluation of the Gulf of California as a potential source of bioactive marine actinobacteria |
| title_alt | Evaluación del golfo de California como una fuente potencial de actinobacterias marinas bioactivas |
| topic | Ciencias de la Tierra cytotoxic antibiotic Salinispora Gulf of California Marine actinobacteria |
| url | https://www.redalyc.org/articulo.oa?id=48025112003 https://cienciasmarinas.com.mx/index.php/cmarinas/article/view/2131 |