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| Format: | Artículo científico |
| Language: | en |
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Universidad Autónoma de Baja California
2000
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| Online Access: | https://www.redalyc.org/articulo.oa?id=48026208 |
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| _version_ | 1866815547439579136 |
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| author | María de Jesús Ferrara Guerrero |
| author_facet | María de Jesús Ferrara Guerrero |
| contents | Microaerophilic bacterial biomass measurements based on adenosine triphosphate and direct counting techniques in a semi-solid agar column María de Jesús Ferrara Guerrero Armand Bianchi Ciencias de la Tierra ATP biomass direct counting microaerophilic bacteria Microaerophilic-diazotrophic bacterial biomass was measured by Adenosine Triphosphate (ATP)extraction and direct counting/biovolume techniques. Microaerophilic bacterial growth in a semi-solidagar column is visible in the form of sharp, well-defined growth rings at different depths within thecolumn according to oxygen requirements. The microaerophilic zone is found between 3 and 15 mmbelow the agar surface, where the dissolved oxygen concentration varies from 4 to 172 µM. Biomassmeasurements calculated using a conventional conversion factor C/ATP of 250 were around ten timeslower than those calculated according to the direct counting/biovolume technique. In the present study,we propose a conversion factor of 356 µg C/mL ATP that gives biomass measurements nearer to thoseobtained by direct counting. The highest bacterial metabolic activity was found between 5 and 10 mmdepth (0.15 ± 0.029 µg ATP mL1), coinciding with optimum oxygen concentration levels for microaerophilicgrowth (6.5 to 114 µM) 2000 artículo científico 0185-3880 https://www.redalyc.org/articulo.oa?id=48026208 en http://www.redalyc.org/revista.oa?id=480 Ciencias Marinas application/pdf Universidad Autónoma de Baja California Ciencias Marinas (México) Num.2 Vol.26 |
| format | Artículo científico |
| id | redalyc_48026208 |
| language | en |
| publishDate | 2000 |
| publisher | Universidad Autónoma de Baja California |
| spellingShingle | Microaerophilic bacterial biomass measurements based on adenosine triphosphate and direct counting techniques in a semi-solid agar column María de Jesús Ferrara Guerrero Ciencias de la Tierra ATP biomass direct counting microaerophilic bacteria Microaerophilic bacterial biomass measurements based on adenosine triphosphate and direct counting techniques in a semi-solid agar column María de Jesús Ferrara Guerrero Armand Bianchi Ciencias de la Tierra ATP biomass direct counting microaerophilic bacteria Microaerophilic-diazotrophic bacterial biomass was measured by Adenosine Triphosphate (ATP)extraction and direct counting/biovolume techniques. Microaerophilic bacterial growth in a semi-solidagar column is visible in the form of sharp, well-defined growth rings at different depths within thecolumn according to oxygen requirements. The microaerophilic zone is found between 3 and 15 mmbelow the agar surface, where the dissolved oxygen concentration varies from 4 to 172 µM. Biomassmeasurements calculated using a conventional conversion factor C/ATP of 250 were around ten timeslower than those calculated according to the direct counting/biovolume technique. In the present study,we propose a conversion factor of 356 µg C/mL ATP that gives biomass measurements nearer to thoseobtained by direct counting. The highest bacterial metabolic activity was found between 5 and 10 mmdepth (0.15 ± 0.029 µg ATP mL1), coinciding with optimum oxygen concentration levels for microaerophilicgrowth (6.5 to 114 µM) 2000 artículo científico 0185-3880 https://www.redalyc.org/articulo.oa?id=48026208 en http://www.redalyc.org/revista.oa?id=480 Ciencias Marinas application/pdf Universidad Autónoma de Baja California Ciencias Marinas (México) Num.2 Vol.26 |
| title | Microaerophilic bacterial biomass measurements based on adenosine triphosphate and direct counting techniques in a semi-solid agar column |
| topic | Ciencias de la Tierra ATP biomass direct counting microaerophilic bacteria |
| url | https://www.redalyc.org/articulo.oa?id=48026208 |