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| Format: | Artículo científico |
| Language: | en |
| Published: |
Universidad Autónoma de Baja California
2000
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| Online Access: | https://www.redalyc.org/articulo.oa?id=48026208 |
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Table of Contents:
- Microaerophilic bacterial biomass measurements based on adenosine triphosphate and direct counting techniques in a semi-solid agar column María de Jesús Ferrara Guerrero Armand Bianchi Ciencias de la Tierra ATP biomass direct counting microaerophilic bacteria Microaerophilic-diazotrophic bacterial biomass was measured by Adenosine Triphosphate (ATP)extraction and direct counting/biovolume techniques. Microaerophilic bacterial growth in a semi-solidagar column is visible in the form of sharp, well-defined growth rings at different depths within thecolumn according to oxygen requirements. The microaerophilic zone is found between 3 and 15 mmbelow the agar surface, where the dissolved oxygen concentration varies from 4 to 172 µM. Biomassmeasurements calculated using a conventional conversion factor C/ATP of 250 were around ten timeslower than those calculated according to the direct counting/biovolume technique. In the present study,we propose a conversion factor of 356 µg C/mL ATP that gives biomass measurements nearer to thoseobtained by direct counting. The highest bacterial metabolic activity was found between 5 and 10 mmdepth (0.15 ± 0.029 µg ATP mL1), coinciding with optimum oxygen concentration levels for microaerophilicgrowth (6.5 to 114 µM) 2000 artículo científico 0185-3880 https://www.redalyc.org/articulo.oa?id=48026208 en http://www.redalyc.org/revista.oa?id=480 Ciencias Marinas application/pdf Universidad Autónoma de Baja California Ciencias Marinas (México) Num.2 Vol.26