Induction of metamorphosis with KCl in three species of sea urchins and its implications in the production of juveniles
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Redalyc · Ciencias Marinas
A common practice for inducing metamorphosis in larvae of marine invertebrates is the use of a wide variety of artificial inducers. Excess K+ and Cs+ in seawater have proved to be very effective for this purpose, but not universally effective to induce metamorphosis in different phyla of marine inve...
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Universidad Autónoma de Baja California
2002
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| author | E. Carpizo Ituarte |
| author_facet | E. Carpizo Ituarte |
| collection | Ciencias Marinas |
| container_title | Ciencias Marinas |
| contents | Induction of metamorphosis with KCl in three species of sea urchins and its implications in the production of juveniles E. Carpizo Ituarte A. Salas Garza G. Parés Sierra Ciencias de la Tierra sea urchin metamorphosis excess potassium signal transduction A common practice for inducing metamorphosis in larvae of marine invertebrates is the use of a wide variety of artificialinducers. Excess K+ and Cs+ in seawater have proved to be very effective for this purpose, but not universally effective to inducemetamorphosis in different phyla of marine invertebrates. In the present study we tested the response of three species of seaurchins to excess K+ in seawater as an artificial inducer. Excess K+ in seawater was presented to the larvae as continuous (24 h) orpulse exposure in concentrations ranging from 0.01 to 100 mM. Results showed that excess K+ in the form of KCl added toseawater was an effective metamorphic inducer of the three species of sea urchins tested; the purple sea urchin Strongylocentrotuspurpuratus, the red sea urchin S. franciscanus and the white sea urchin Lytechinus pictus. The response was dependent onconcentration and time of exposure. Continuous exposure (24 h or more) to excess K+ (100 mM) resulted lethal to the larvae ofthe three species. Excess K+ (100 mM) for 15 min resulted the most effective pulse-period to induce metamorphosis in the threespecies. This method of induction allows us to produce synchronous metamorphosed postlarvae useful for aquaculture and studiesof signal transduction mechanisms during metamorphosis of sea urchins. 2002 artículo científico 0185-3880 https://www.redalyc.org/articulo.oa?id=48028204 en http://www.redalyc.org/revista.oa?id=480 Ciencias Marinas application/pdf Universidad Autónoma de Baja California Ciencias Marinas (México) Num.2 Vol.28 |
| ctrlnum | oai:cienciasmarinas.com.mx:article/217 10.7773/cm.v28i2.217 |
| description | A common practice for inducing metamorphosis in larvae of marine invertebrates is the use of a wide variety of artificial inducers. Excess K+ and Cs+ in seawater have proved to be very effective for this purpose, but not universally effective to induce metamorphosis in different phyla of marine invertebrates. In the present study we tested the response of three species of sea urchins to excess K+ in seawater as an artificial inducer. Excess K+ in seawater was presented to the larvae as continuous (24 h) or pulse exposure in concentrations ranging from 0.01 to 100 mM. Results showed that excess K+ in the form of KCl added to seawater was an effective metamorphic inducer of the three species of sea urchins tested; the purple sea urchin Strongylocentrotus purpuratus, the red sea urchin S. franciscanus and the white sea urchin Lytechinus pictus. The response was dependent on concentration and time of exposure. Continuous exposure (24 h or more) to excess K+ (100 mM) resulted lethal to the larvae of the three species. Excess K+ (100 mM) for 15 min resulted the most effective pulse-period to induce metamorphosis in the three species. This method of induction allows us to produce synchronous metamorphosed postlarvae useful for aquaculture and studies of signal transduction mechanisms during metamorphosis of sea urchins.
La utilización de inductores artificiales es una práctica común para inducir la metamorfosis en larvas de invertebrados marinos. Potasio (K+) en exceso y Cs+ en el agua de mar, han demostrado ser muy efectivos para estos propósitos, pero no universalmente efectivos para inducir la metamorfosis en diversos fila de invertebrados marinos. En el presente estudio, se puso a prueba la respuesta de tres especies de erizo de mar en presencia de exceso de K+ en el agua de mar, como inductor artificial. Las larvas fueron expuestas a un exceso de K+ en el agua de mar en forma continua (24 h) o en pulsos de concentraciones de 0.01 a 100 mM. Los resultados mostraron que el exceso de K+ agregado al agua de mar en forma de KCl fue un inductor efectivo para las tres especies de erizo, el erizo morado Strongylocentrotus purpuratus, el rojo S. franciscanus y el blanco Lytechinus pictus. La respuesta fue en función de la concentración y el tiempo de exposición. Las larvas de las tres especies murieron cuando fueron expuestas en forma continua (24 h o más) al exceso de K+ (100 mM). La exposición a un exceso de K+ (100 mM) por 15 minutos resultó la forma más efectiva para inducir la metamorfosis en las tres especies. Este método de inducción permite la obtención de poslarvas que se metamorfizan en forma sincrónica, y resulta útil para fines acuaculturales o para estudios relacionados con rutas de transducción y expresión genética durante la metamorfosis de erizos de mar. |
| format | Artículo científico |
| id | redalyc_48028204 |
| institution | Redalyc Ciencias Marinas |
| language | en |
| publishDate | 2002 |
| publisher | Universidad Autónoma de Baja California |
| spelling | Induction of metamorphosis with KCl in three species of sea urchins and its implications in the production of juveniles Inducción de la metamorfosis con KCl en tres especies de erizos de mar y sus implicaciones en la producción de juveniles Carpizo-Ituarte, E Salas-Garza, A Parés-Sierra, G metamorphosis sea urchin excess potassium signal transduction metamorfosis erizo de mar exceso de potasio rutas de transducción A common practice for inducing metamorphosis in larvae of marine invertebrates is the use of a wide variety of artificial inducers. Excess K+ and Cs+ in seawater have proved to be very effective for this purpose, but not universally effective to induce metamorphosis in different phyla of marine invertebrates. In the present study we tested the response of three species of sea urchins to excess K+ in seawater as an artificial inducer. Excess K+ in seawater was presented to the larvae as continuous (24 h) or pulse exposure in concentrations ranging from 0.01 to 100 mM. Results showed that excess K+ in the form of KCl added to seawater was an effective metamorphic inducer of the three species of sea urchins tested; the purple sea urchin Strongylocentrotus purpuratus, the red sea urchin S. franciscanus and the white sea urchin Lytechinus pictus. The response was dependent on concentration and time of exposure. Continuous exposure (24 h or more) to excess K+ (100 mM) resulted lethal to the larvae of the three species. Excess K+ (100 mM) for 15 min resulted the most effective pulse-period to induce metamorphosis in the three species. This method of induction allows us to produce synchronous metamorphosed postlarvae useful for aquaculture and studies of signal transduction mechanisms during metamorphosis of sea urchins. La utilización de inductores artificiales es una práctica común para inducir la metamorfosis en larvas de invertebrados marinos. Potasio (K+) en exceso y Cs+ en el agua de mar, han demostrado ser muy efectivos para estos propósitos, pero no universalmente efectivos para inducir la metamorfosis en diversos fila de invertebrados marinos. En el presente estudio, se puso a prueba la respuesta de tres especies de erizo de mar en presencia de exceso de K+ en el agua de mar, como inductor artificial. Las larvas fueron expuestas a un exceso de K+ en el agua de mar en forma continua (24 h) o en pulsos de concentraciones de 0.01 a 100 mM. Los resultados mostraron que el exceso de K+ agregado al agua de mar en forma de KCl fue un inductor efectivo para las tres especies de erizo, el erizo morado Strongylocentrotus purpuratus, el rojo S. franciscanus y el blanco Lytechinus pictus. La respuesta fue en función de la concentración y el tiempo de exposición. Las larvas de las tres especies murieron cuando fueron expuestas en forma continua (24 h o más) al exceso de K+ (100 mM). La exposición a un exceso de K+ (100 mM) por 15 minutos resultó la forma más efectiva para inducir la metamorfosis en las tres especies. Este método de inducción permite la obtención de poslarvas que se metamorfizan en forma sincrónica, y resulta útil para fines acuaculturales o para estudios relacionados con rutas de transducción y expresión genética durante la metamorfosis de erizos de mar. Universidad Autónoma de Baja California, Instituto de Investigaciones Oceanológicas 2002-03-06 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article Artículo Arbitrado application/pdf https://cienciasmarinas.com.mx/index.php/cmarinas/article/view/217 10.7773/cm.v28i2.217 Ciencias Marinas; Vol. 28 No. 2 (2002); 157-166 Ciencias Marinas; Vol. 28 Núm. 2 (2002); 157-166 2395-9053 0185-3880 eng https://cienciasmarinas.com.mx/index.php/cmarinas/article/view/217/184 ['Redalyc'] redalyc_48028204 Induction of metamorphosis with KCl in three species of sea urchins and its implications in the production of juveniles ['Induction of metamorphosis with KCl in three species of sea urchins and its implications in the production of juveniles', 'E. Carpizo Ituarte', 'Ciencias de la Tierra', 'sea urchin', 'metamorphosis', 'excess potassium', 'signal transduction', 'Induction of metamorphosis with KCl in three species of sea urchins and its implications in the production of juveniles E. Carpizo Ituarte A. Salas Garza G. Parés Sierra Ciencias de la Tierra sea urchin metamorphosis excess potassium signal transduction A common practice for inducing metamorphosis in larvae of marine invertebrates is the use of a wide variety of artificialinducers. Excess K+ and Cs+ in seawater have proved to be very effective for this purpose, but not universally effective to inducemetamorphosis in different phyla of marine invertebrates. In the present study we tested the response of three species of seaurchins to excess K+ in seawater as an artificial inducer. Excess K+ in seawater was presented to the larvae as continuous (24 h) orpulse exposure in concentrations ranging from 0.01 to 100 mM. Results showed that excess K+ in the form of KCl added toseawater was an effective metamorphic inducer of the three species of sea urchins tested; the purple sea urchin Strongylocentrotuspurpuratus, the red sea urchin S. franciscanus and the white sea urchin Lytechinus pictus. The response was dependent onconcentration and time of exposure. Continuous exposure (24 h or more) to excess K+ (100 mM) resulted lethal to the larvae ofthe three species. Excess K+ (100 mM) for 15 min resulted the most effective pulse-period to induce metamorphosis in the threespecies. This method of induction allows us to produce synchronous metamorphosed postlarvae useful for aquaculture and studiesof signal transduction mechanisms during metamorphosis of sea urchins. 2002 artículo científico 0185-3880 https://www.redalyc.org/articulo.oa?id=48028204 en http://www.redalyc.org/revista.oa?id=480 Ciencias Marinas application/pdf Universidad Autónoma de Baja California Ciencias Marinas (México) Num.2 Vol.28'] ['E. Carpizo Ituarte'] ['E. Carpizo Ituarte'] ['Artículo científico'] ['2002'] ['Universidad Autónoma de Baja California'] ['en'] ['Ciencias de la Tierra', 'sea urchin', 'metamorphosis', 'excess potassium', 'signal transduction'] ['https://www.redalyc.org/articulo.oa?id=48028204'] ['Induction of metamorphosis with KCl in three species of sea urchins and its implications in the production of juveniles E. Carpizo Ituarte A. Salas Garza G. Parés Sierra Ciencias de la Tierra sea urchin metamorphosis excess potassium signal transduction A common practice for inducing metamorphosis in larvae of marine invertebrates is the use of a wide variety of artificialinducers. Excess K+ and Cs+ in seawater have proved to be very effective for this purpose, but not universally effective to inducemetamorphosis in different phyla of marine invertebrates. In the present study we tested the response of three species of seaurchins to excess K+ in seawater as an artificial inducer. Excess K+ in seawater was presented to the larvae as continuous (24 h) orpulse exposure in concentrations ranging from 0.01 to 100 mM. Results showed that excess K+ in the form of KCl added toseawater was an effective metamorphic inducer of the three species of sea urchins tested; the purple sea urchin Strongylocentrotuspurpuratus, the red sea urchin S. franciscanus and the white sea urchin Lytechinus pictus. The response was dependent onconcentration and time of exposure. Continuous exposure (24 h or more) to excess K+ (100 mM) resulted lethal to the larvae ofthe three species. Excess K+ (100 mM) for 15 min resulted the most effective pulse-period to induce metamorphosis in the threespecies. This method of induction allows us to produce synchronous metamorphosed postlarvae useful for aquaculture and studiesof signal transduction mechanisms during metamorphosis of sea urchins. 2002 artículo científico 0185-3880 https://www.redalyc.org/articulo.oa?id=48028204 en http://www.redalyc.org/revista.oa?id=480 Ciencias Marinas application/pdf Universidad Autónoma de Baja California Ciencias Marinas (México) Num.2 Vol.28'] |
| spellingShingle | Induction of metamorphosis with KCl in three species of sea urchins and its implications in the production of juveniles E. Carpizo Ituarte Ciencias de la Tierra sea urchin metamorphosis excess potassium signal transduction Induction of metamorphosis with KCl in three species of sea urchins and its implications in the production of juveniles E. Carpizo Ituarte A. Salas Garza G. Parés Sierra Ciencias de la Tierra sea urchin metamorphosis excess potassium signal transduction A common practice for inducing metamorphosis in larvae of marine invertebrates is the use of a wide variety of artificialinducers. Excess K+ and Cs+ in seawater have proved to be very effective for this purpose, but not universally effective to inducemetamorphosis in different phyla of marine invertebrates. In the present study we tested the response of three species of seaurchins to excess K+ in seawater as an artificial inducer. Excess K+ in seawater was presented to the larvae as continuous (24 h) orpulse exposure in concentrations ranging from 0.01 to 100 mM. Results showed that excess K+ in the form of KCl added toseawater was an effective metamorphic inducer of the three species of sea urchins tested; the purple sea urchin Strongylocentrotuspurpuratus, the red sea urchin S. franciscanus and the white sea urchin Lytechinus pictus. The response was dependent onconcentration and time of exposure. Continuous exposure (24 h or more) to excess K+ (100 mM) resulted lethal to the larvae ofthe three species. Excess K+ (100 mM) for 15 min resulted the most effective pulse-period to induce metamorphosis in the threespecies. This method of induction allows us to produce synchronous metamorphosed postlarvae useful for aquaculture and studiesof signal transduction mechanisms during metamorphosis of sea urchins. 2002 artículo científico 0185-3880 https://www.redalyc.org/articulo.oa?id=48028204 en http://www.redalyc.org/revista.oa?id=480 Ciencias Marinas application/pdf Universidad Autónoma de Baja California Ciencias Marinas (México) Num.2 Vol.28 |
| title | Induction of metamorphosis with KCl in three species of sea urchins and its implications in the production of juveniles |
| title_alt | Inducción de la metamorfosis con KCl en tres especies de erizos de mar y sus implicaciones en la producción de juveniles |
| topic | Ciencias de la Tierra sea urchin metamorphosis excess potassium signal transduction |
| topic_facet | metamorphosis sea urchin excess potassium signal transduction metamorfosis erizo de mar exceso de potasio rutas de transducción |
| url | https://www.redalyc.org/articulo.oa?id=48028204 https://cienciasmarinas.com.mx/index.php/cmarinas/article/view/217 |