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| Natura: | Artículo científico |
| Lingua: | en |
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Universidad Autónoma de Baja California
2002
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| Accesso online: | https://www.redalyc.org/articulo.oa?id=48028409 |
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| _version_ | 1866815550301143040 |
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| author | Carmen Piñuela |
| author_facet | Carmen Piñuela |
| contents | Histochemical characteristics of the vitellogenic oocytes of the bluefin tuna, Thunnus thynnus L. Carmen Piñuela J. Antonio Muñoz Cueto Carmen Rendón Evaristo L. Mañanós F. J. Rodríguez Gómez Emilio Pascual Carmen Sarasquete M. Luisa González de Canales Ciencias de la Tierra tuna ovary lipids proteins vitellogenin Very little information is available with respect to dynamic aspects of oocyte development and to yolk composition of tunaspecies. Since histological approaches can offer considerable information about the ovarian development of fish, and becausedifferent cytochemical/immunohistochemical tests are suitable tools for proving the presence of different macromolecules inseveral organs/tissues/cells, they can be good biomarkers of the reproductive mechanism in fish. The present study investigatesthe presence and distribution of carbohydrates, proteins, glycoconjugates, lipids, calcium, iron and vitellogenin in the bluefintuna, Thunnus thynnus oocytes during the synthesis of yolk (globules and granules) in the vitellogenic phase. During the initialvitellogenic phase, oocytes (diameter: 249 ± 21.87 mm) contain oil globules (or vacuoles) which consist exclusively of neutrallipids. During this phase very small yolk granules appear around the cytoplasm. Glycolipids, phospholipids, glycoproteins (Nacetyl-D-galactosamine GalNAc sugar residues), calcium, proteins rich in tyrosine, tryptophan, lysine, arginine, cysteine andcystine, as well as vitellogenin are the main constituents of the yolk granules and the follicular envelope of these real vitellogenicoocytes (diameter: 386 ± 79.04 mm). The intergranular cytoplasm of the vitellogenic oocytes also contain glycogen andglycoconjugates with Mannose Man and/or Glucose Glc; N-acetyl-D-galactosamine GalNAc; L-Fucose Fuc and Nacetyl-D-glucosamine GlcNAc and/or sialic acid NANA sugar residues. The zona radiata is mainly composed of proteins;in this cellular layer, scarce neutral glycoproteins and lipids (phospholipids/glycolipids) are also present 2002 artículo científico 0185-3880 https://www.redalyc.org/articulo.oa?id=48028409 en http://www.redalyc.org/revista.oa?id=480 Ciencias Marinas application/pdf Universidad Autónoma de Baja California Ciencias Marinas (México) Num.4 Vol.28 |
| format | Artículo científico |
| id | redalyc_48028409 |
| language | en |
| publishDate | 2002 |
| publisher | Universidad Autónoma de Baja California |
| spellingShingle | Histochemical characteristics of the vitellogenic oocytes of the bluefin tuna, Thunnus thynnus L. Carmen Piñuela Ciencias de la Tierra tuna ovary lipids proteins vitellogenin Histochemical characteristics of the vitellogenic oocytes of the bluefin tuna, Thunnus thynnus L. Carmen Piñuela J. Antonio Muñoz Cueto Carmen Rendón Evaristo L. Mañanós F. J. Rodríguez Gómez Emilio Pascual Carmen Sarasquete M. Luisa González de Canales Ciencias de la Tierra tuna ovary lipids proteins vitellogenin Very little information is available with respect to dynamic aspects of oocyte development and to yolk composition of tunaspecies. Since histological approaches can offer considerable information about the ovarian development of fish, and becausedifferent cytochemical/immunohistochemical tests are suitable tools for proving the presence of different macromolecules inseveral organs/tissues/cells, they can be good biomarkers of the reproductive mechanism in fish. The present study investigatesthe presence and distribution of carbohydrates, proteins, glycoconjugates, lipids, calcium, iron and vitellogenin in the bluefintuna, Thunnus thynnus oocytes during the synthesis of yolk (globules and granules) in the vitellogenic phase. During the initialvitellogenic phase, oocytes (diameter: 249 ± 21.87 mm) contain oil globules (or vacuoles) which consist exclusively of neutrallipids. During this phase very small yolk granules appear around the cytoplasm. Glycolipids, phospholipids, glycoproteins (Nacetyl-D-galactosamine GalNAc sugar residues), calcium, proteins rich in tyrosine, tryptophan, lysine, arginine, cysteine andcystine, as well as vitellogenin are the main constituents of the yolk granules and the follicular envelope of these real vitellogenicoocytes (diameter: 386 ± 79.04 mm). The intergranular cytoplasm of the vitellogenic oocytes also contain glycogen andglycoconjugates with Mannose Man and/or Glucose Glc; N-acetyl-D-galactosamine GalNAc; L-Fucose Fuc and Nacetyl-D-glucosamine GlcNAc and/or sialic acid NANA sugar residues. The zona radiata is mainly composed of proteins;in this cellular layer, scarce neutral glycoproteins and lipids (phospholipids/glycolipids) are also present 2002 artículo científico 0185-3880 https://www.redalyc.org/articulo.oa?id=48028409 en http://www.redalyc.org/revista.oa?id=480 Ciencias Marinas application/pdf Universidad Autónoma de Baja California Ciencias Marinas (México) Num.4 Vol.28 |
| title | Histochemical characteristics of the vitellogenic oocytes of the bluefin tuna, Thunnus thynnus L. |
| topic | Ciencias de la Tierra tuna ovary lipids proteins vitellogenin |
| url | https://www.redalyc.org/articulo.oa?id=48028409 |