Genetic connectivity of Stegastes partitus in the South Caribbean evidenced by microsatellite analysis
Fuente:
Redalyc · Ciencias Marinas
Bicolor damselfish Stegastes partitus (Poey 1986) has been used as a model for studies of the population structure of reef species as an aid for the designing and monitoring of marine protected areas. Studies using allozymes have shown gene flow between populations, but it has been suggested that th...
Saved in:
| Published in: | Ciencias Marinas |
|---|---|
| Main Author: | |
| Format: | Artículo científico |
| Language: | en |
| Published: |
Universidad Autónoma de Baja California
2008
|
| Subjects: | |
| Online Access: | |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1878325299525976064 |
|---|---|
| author | SP Ospina-Guerrero |
| author_facet | SP Ospina-Guerrero |
| collection | Ciencias Marinas |
| container_title | Ciencias Marinas |
| contents | Genetic connectivity of Stegastes partitus in the South Caribbean evidenced by microsatellite analysis SP Ospina-Guerrero RM Landinez-García DJ Rodríguez-Castro R Arango E Márquez Ciencias de la Tierra Gene flow genetic diversity bicolor damselfish marine protected areas Bicolor damselfish Stegastes partitus (Poey 1986) has been used as a model for studies of the population structure of reef species as an aid for the designing and monitoring of marine protected areas. Studies using allozymes have shown gene flow between populations, but it has been suggested that the lack of structuring could be a consequence of the insensitivity of these markers. We used 11 polymorphic microsatellite loci to evaluate the genetic connectivity of 299 bicolor damselfish from four representative areas of the Colombian coast in the South Caribbean (Santa Marta, Rosario Islands, Capurganá, and San Andrés Island). Genotyping was made using detection by fluorescence of multiple alleles. Genetic differentiation among geographic populations and different oceanographic scenarios was analyzed using the statistic ΦST and analysis of molecular variance. The correlation between genetic and geographic distance was explored using the Mantel test. All loci were polymorphic, and showed a high number of alleles per locus and heterozygosity deficit with a consequent departure from Hardy-Weinberg equilibrium. We found evidence of gene flow between the geographic and oceanographic populations examined and a lack of correlation between the genetic and geographic distances. The information obtained can be used by the agencies responsible for the design and conservation of marine protected areas and as reference in the monitoring of the genetic diversity and structure of the population under study. 2008 artículo científico 0185-3880 https://www.redalyc.org/articulo.oa?id=48030191002 en http://www.redalyc.org/revista.oa?id=480 Ciencias Marinas application/pdf Universidad Autónoma de Baja California Ciencias Marinas (México) Num.2 Vol.34 |
| ctrlnum | oai:cienciasmarinas.com.mx:article/1237 10.7773/cm.v34i2.1237 |
| description | Bicolor damselfish Stegastes partitus (Poey 1986) has been used as a model for studies of the population structure of reef species as an aid for the designing and monitoring of marine protected areas. Studies using allozymes have shown gene flow between populations, but it has been suggested that the lack of structuring could be a consequence of the insensitivity of these markers. We used 11 polymorphic microsatellite loci to evaluate the genetic connectivity of 299 bicolor damselfish from four representative areas of the Colombian coast in the South Caribbean (Santa Marta, Rosario Islands, Capurganá, and San Andrés Island). Genotyping was made using detection by fluorescence of multiple alleles. Genetic differentiation among geographic populations and different oceanographic scenarios was analyzed using the statistic ΦST and analysis of molecular variance. The correlation between genetic and geographic distance was explored using the Mantel test. All loci were polymorphic, and showed a high number of alleles per locus and heterozygosity deficit with a consequent departure from Hardy-Weinberg equilibrium. We found evidence of gene flow between the geographic and oceanographic populations examined and a lack of correlation between the genetic and geographic distances. The information obtained can be used by the agencies responsible for the design and conservation of marine protected areas and as reference in the monitoring of the genetic diversity and structure of the population under study.
La damisela bicolor Stegastes partitus (Poey 1986) ha sido utilizada como modelo en estudios de estructura poblacional de especies arrecifales para el diseño y monitoreo de áreas marinas protegidas. Los estudios con aloenzimas reflejan flujo genético entre poblaciones pero se ha sugerido que la ausencia de estructuración puede ser una consecuencia de la insensibilidad de los marcadores utilizados. En el presente trabajo se utilizaron 11 loci microsatélites polimórficos para evaluar la conectividad genética de la damisela bicolor en 299 individuos provenientes de cuatro áreas representativas del sur del Mar Caribe en Colombia (Santa Marta, Islas del Rosario, Capurgana e Isla de San Andrés). La genotipificación se realizó adoptando la metodología de detección por fluorescencia de múltiples alelos. La diferenciación genética entre las poblaciones geográficas y entre los diferentes escenarios oceanográficos se analizó utilizando el estadístico ΦST y el análisis de varianza molecular. La correlación entre la distancia genética y la distancia geográfica se exploró mediante el uso de la prueba de Mantel. Todos los loci fueron polimórficos, presentaron elevado número de alelos por locus y déficit de heterocigosidad con consecuente desviación del equilibrio Hardy-Weinberg. Se encontró evidencia de flujo genético entre las poblaciones geográficas y entre los escenarios oceanográficos analizados y ausencia de correlación entre la distancia genética y la geográfica. La información obtenida puede ser utilizada por las instancias encargadas del diseño y la conservación de áreas marinas protegidas y constituye un punto de partida para el seguimiento de la diversidad genética y la estructura de las poblaciones bajo estudio. |
| format | Artículo científico |
| id | redalyc_48030191002 |
| institution | Redalyc Ciencias Marinas |
| language | en |
| publishDate | 2008 |
| publisher | Universidad Autónoma de Baja California |
| spelling | Genetic connectivity of Stegastes partitus in the South Caribbean evidenced by microsatellite analysis Conectividad genética de Stegastes partitus en el Caribe Sur evidenciada por análisis microsatélite Ospina-Guerrero, SP Landnez-García, RM Rodríguez-Castro, DJ Arango, R Márquez, E gene flow genetic diversity marine protected areas bicolor damselfish flujo genético diversidad genética areas marinas protegidas damisela bicolor Bicolor damselfish Stegastes partitus (Poey 1986) has been used as a model for studies of the population structure of reef species as an aid for the designing and monitoring of marine protected areas. Studies using allozymes have shown gene flow between populations, but it has been suggested that the lack of structuring could be a consequence of the insensitivity of these markers. We used 11 polymorphic microsatellite loci to evaluate the genetic connectivity of 299 bicolor damselfish from four representative areas of the Colombian coast in the South Caribbean (Santa Marta, Rosario Islands, Capurganá, and San Andrés Island). Genotyping was made using detection by fluorescence of multiple alleles. Genetic differentiation among geographic populations and different oceanographic scenarios was analyzed using the statistic ΦST and analysis of molecular variance. The correlation between genetic and geographic distance was explored using the Mantel test. All loci were polymorphic, and showed a high number of alleles per locus and heterozygosity deficit with a consequent departure from Hardy-Weinberg equilibrium. We found evidence of gene flow between the geographic and oceanographic populations examined and a lack of correlation between the genetic and geographic distances. The information obtained can be used by the agencies responsible for the design and conservation of marine protected areas and as reference in the monitoring of the genetic diversity and structure of the population under study. La damisela bicolor Stegastes partitus (Poey 1986) ha sido utilizada como modelo en estudios de estructura poblacional de especies arrecifales para el diseño y monitoreo de áreas marinas protegidas. Los estudios con aloenzimas reflejan flujo genético entre poblaciones pero se ha sugerido que la ausencia de estructuración puede ser una consecuencia de la insensibilidad de los marcadores utilizados. En el presente trabajo se utilizaron 11 loci microsatélites polimórficos para evaluar la conectividad genética de la damisela bicolor en 299 individuos provenientes de cuatro áreas representativas del sur del Mar Caribe en Colombia (Santa Marta, Islas del Rosario, Capurgana e Isla de San Andrés). La genotipificación se realizó adoptando la metodología de detección por fluorescencia de múltiples alelos. La diferenciación genética entre las poblaciones geográficas y entre los diferentes escenarios oceanográficos se analizó utilizando el estadístico ΦST y el análisis de varianza molecular. La correlación entre la distancia genética y la distancia geográfica se exploró mediante el uso de la prueba de Mantel. Todos los loci fueron polimórficos, presentaron elevado número de alelos por locus y déficit de heterocigosidad con consecuente desviación del equilibrio Hardy-Weinberg. Se encontró evidencia de flujo genético entre las poblaciones geográficas y entre los escenarios oceanográficos analizados y ausencia de correlación entre la distancia genética y la geográfica. La información obtenida puede ser utilizada por las instancias encargadas del diseño y la conservación de áreas marinas protegidas y constituye un punto de partida para el seguimiento de la diversidad genética y la estructura de las poblaciones bajo estudio. Universidad Autónoma de Baja California, Instituto de Investigaciones Oceanológicas 2008-06-05 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article Artículo Arbitrado application/pdf https://cienciasmarinas.com.mx/index.php/cmarinas/article/view/1237 10.7773/cm.v34i2.1237 Ciencias Marinas; Vol. 34 No. 2 (2008); 155–163 Ciencias Marinas; Vol. 34 Núm. 2 (2008); 155–163 2395-9053 0185-3880 eng https://cienciasmarinas.com.mx/index.php/cmarinas/article/view/1237/1843 ['Redalyc'] redalyc_48030191002 Genetic connectivity of Stegastes partitus in the South Caribbean evidenced by microsatellite analysis ['Genetic connectivity of Stegastes partitus in the South Caribbean evidenced by microsatellite analysis', 'SP Ospina-Guerrero', 'Ciencias de la Tierra', 'Gene flow', 'genetic diversity', 'bicolor damselfish', 'marine protected areas', 'Genetic connectivity of Stegastes partitus in the South Caribbean evidenced by microsatellite analysis SP Ospina-Guerrero RM Landinez-García DJ Rodríguez-Castro R Arango E Márquez Ciencias de la Tierra Gene flow genetic diversity bicolor damselfish marine protected areas Bicolor damselfish Stegastes partitus (Poey 1986) has been used as a model for studies of the population structure of reef species as an aid for the designing and monitoring of marine protected areas. Studies using allozymes have shown gene flow between populations, but it has been suggested that the lack of structuring could be a consequence of the insensitivity of these markers. We used 11 polymorphic microsatellite loci to evaluate the genetic connectivity of 299 bicolor damselfish from four representative areas of the Colombian coast in the South Caribbean (Santa Marta, Rosario Islands, Capurganá, and San Andrés Island). Genotyping was made using detection by fluorescence of multiple alleles. Genetic differentiation among geographic populations and different oceanographic scenarios was analyzed using the statistic ΦST and analysis of molecular variance. The correlation between genetic and geographic distance was explored using the Mantel test. All loci were polymorphic, and showed a high number of alleles per locus and heterozygosity deficit with a consequent departure from Hardy-Weinberg equilibrium. We found evidence of gene flow between the geographic and oceanographic populations examined and a lack of correlation between the genetic and geographic distances. The information obtained can be used by the agencies responsible for the design and conservation of marine protected areas and as reference in the monitoring of the genetic diversity and structure of the population under study. 2008 artículo científico 0185-3880 https://www.redalyc.org/articulo.oa?id=48030191002 en http://www.redalyc.org/revista.oa?id=480 Ciencias Marinas application/pdf Universidad Autónoma de Baja California Ciencias Marinas (México) Num.2 Vol.34'] ['SP Ospina-Guerrero'] ['SP Ospina-Guerrero'] ['Artículo científico'] ['2008'] ['Universidad Autónoma de Baja California'] ['en'] ['Ciencias de la Tierra', 'Gene flow', 'genetic diversity', 'bicolor damselfish', 'marine protected areas'] ['https://www.redalyc.org/articulo.oa?id=48030191002'] ['Genetic connectivity of Stegastes partitus in the South Caribbean evidenced by microsatellite analysis SP Ospina-Guerrero RM Landinez-García DJ Rodríguez-Castro R Arango E Márquez Ciencias de la Tierra Gene flow genetic diversity bicolor damselfish marine protected areas Bicolor damselfish Stegastes partitus (Poey 1986) has been used as a model for studies of the population structure of reef species as an aid for the designing and monitoring of marine protected areas. Studies using allozymes have shown gene flow between populations, but it has been suggested that the lack of structuring could be a consequence of the insensitivity of these markers. We used 11 polymorphic microsatellite loci to evaluate the genetic connectivity of 299 bicolor damselfish from four representative areas of the Colombian coast in the South Caribbean (Santa Marta, Rosario Islands, Capurganá, and San Andrés Island). Genotyping was made using detection by fluorescence of multiple alleles. Genetic differentiation among geographic populations and different oceanographic scenarios was analyzed using the statistic ΦST and analysis of molecular variance. The correlation between genetic and geographic distance was explored using the Mantel test. All loci were polymorphic, and showed a high number of alleles per locus and heterozygosity deficit with a consequent departure from Hardy-Weinberg equilibrium. We found evidence of gene flow between the geographic and oceanographic populations examined and a lack of correlation between the genetic and geographic distances. The information obtained can be used by the agencies responsible for the design and conservation of marine protected areas and as reference in the monitoring of the genetic diversity and structure of the population under study. 2008 artículo científico 0185-3880 https://www.redalyc.org/articulo.oa?id=48030191002 en http://www.redalyc.org/revista.oa?id=480 Ciencias Marinas application/pdf Universidad Autónoma de Baja California Ciencias Marinas (México) Num.2 Vol.34'] |
| spellingShingle | Genetic connectivity of Stegastes partitus in the South Caribbean evidenced by microsatellite analysis SP Ospina-Guerrero Ciencias de la Tierra Gene flow genetic diversity bicolor damselfish marine protected areas Genetic connectivity of Stegastes partitus in the South Caribbean evidenced by microsatellite analysis SP Ospina-Guerrero RM Landinez-García DJ Rodríguez-Castro R Arango E Márquez Ciencias de la Tierra Gene flow genetic diversity bicolor damselfish marine protected areas Bicolor damselfish Stegastes partitus (Poey 1986) has been used as a model for studies of the population structure of reef species as an aid for the designing and monitoring of marine protected areas. Studies using allozymes have shown gene flow between populations, but it has been suggested that the lack of structuring could be a consequence of the insensitivity of these markers. We used 11 polymorphic microsatellite loci to evaluate the genetic connectivity of 299 bicolor damselfish from four representative areas of the Colombian coast in the South Caribbean (Santa Marta, Rosario Islands, Capurganá, and San Andrés Island). Genotyping was made using detection by fluorescence of multiple alleles. Genetic differentiation among geographic populations and different oceanographic scenarios was analyzed using the statistic ΦST and analysis of molecular variance. The correlation between genetic and geographic distance was explored using the Mantel test. All loci were polymorphic, and showed a high number of alleles per locus and heterozygosity deficit with a consequent departure from Hardy-Weinberg equilibrium. We found evidence of gene flow between the geographic and oceanographic populations examined and a lack of correlation between the genetic and geographic distances. The information obtained can be used by the agencies responsible for the design and conservation of marine protected areas and as reference in the monitoring of the genetic diversity and structure of the population under study. 2008 artículo científico 0185-3880 https://www.redalyc.org/articulo.oa?id=48030191002 en http://www.redalyc.org/revista.oa?id=480 Ciencias Marinas application/pdf Universidad Autónoma de Baja California Ciencias Marinas (México) Num.2 Vol.34 |
| title | Genetic connectivity of Stegastes partitus in the South Caribbean evidenced by microsatellite analysis |
| title_alt | Conectividad genética de Stegastes partitus en el Caribe Sur evidenciada por análisis microsatélite |
| topic | Ciencias de la Tierra Gene flow genetic diversity bicolor damselfish marine protected areas |
| topic_facet | gene flow genetic diversity marine protected areas bicolor damselfish flujo genético diversidad genética areas marinas protegidas damisela bicolor |
| url | https://www.redalyc.org/articulo.oa?id=48030191002 https://cienciasmarinas.com.mx/index.php/cmarinas/article/view/1237 |