A technique for the separation of the most recently deposited nacreous layer in Mytilus californianus shells for trace metal analysis
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Redalyc · Ciencias Marinas
A new method to separate a section of the most recently deposited nacreous layer from the rest of the shell of Mytilus californianus was developed. Techniques described for other mussel species proved unsuitable for this species. In this new method, the shells are soaked in 30% hydrogen peroxide for...
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| Veröffentlicht in: | Ciencias Marinas |
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| Format: | Artículo científico |
| Sprache: | en |
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Universidad Autónoma de Baja California
2004
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| _version_ | 1878325298423922688 |
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| author | L. E. Rivero |
| author_facet | L. E. Rivero |
| collection | Ciencias Marinas |
| container_title | Ciencias Marinas |
| contents | A technique for the separation of the most recently deposited nacreous layer in Mytilus californianus shells for trace metal analysis L. E. Rivero M. L. Lares Ciencias de la Tierra shell cadmium nacreous layer Mytilus californianus A new method to separate a section of the most recently deposited nacreous layer from the rest of the shell of Mytiluscalifornianus was developed. Techniques described for other mussel species proved unsuitable for this species. In this newmethod, the shells are soaked in 30% hydrogen peroxide for 1015 min at 8085ºC to eliminate the periostracum, a potentialsource of trace metal contamination. Next, the shells are heated in a muffle furnace at 350ºC for 1 h to separate the nacreous layerfrom the calcite layer. Finally, the nacreous band is easily separated from the rest of the shell by gently tapping it with a stainlesssteel needle. This is a simple and fast method that is proposed for trace metal monitoring purposes using M. californianus shells.To illustrate the application of the method, results from an experiment performed in our laboratory, in which mussels wereexposed to two different Cd concentrations for 60 days and their shells processed by this technique, are included. The mostrecently deposited nacreous layer showed significant increases in Cd concentrations, which were proportional to the exposedambient concentrations. 2004 artículo científico 0185-3880 https://www.redalyc.org/articulo.oa?id=48030207 en http://www.redalyc.org/revista.oa?id=480 Ciencias Marinas application/pdf Universidad Autónoma de Baja California Ciencias Marinas (México) Num.2 Vol.30 |
| ctrlnum | oai:cienciasmarinas.com.mx:article/181 10.7773/cm.v30i2.181 |
| description | A new method to separate a section of the most recently deposited nacreous layer from the rest of the shell of Mytilus californianus was developed. Techniques described for other mussel species proved unsuitable for this species. In this new method, the shells are soaked in 30% hydrogen peroxide for 10–15 min at 80–85ºC to eliminate the periostracum, a potential source of trace metal contamination. Next, the shells are heated in a muffle furnace at 350ºC for 1 h to separate the nacreous layer from the calcite layer. Finally, the nacreous band is easily separated from the rest of the shell by gently tapping it with a stainless steel needle. This is a simple and fast method that is proposed for trace metal monitoring purposes using M. californianus shells. To illustrate the application of the method, results from an experiment performed in our laboratory, in which mussels were exposed to two different Cd concentrations for 60 days and their shells processed by this technique, are included. The most recently deposited nacreous layer showed significant increases in Cd concentrations, which were proportional to the exposed ambient concentrations.
Se desarrolló un nuevo método para separar una sección de la capa de nácar recientemente depositada del resto de la concha de Mytilus californianus. Los métodos descritos para otras especies de mejillones demostraron no ser apropiados para las conchas de esta especie. En este nuevo método, las conchas son sumergidas en peróxido de hidrógeno al 30% por 10–15 min a 80–85ºC para eliminar el periostraco, una fuente potencial de contaminación por metales traza. Enseguida, las conchas son calentadas en una mufla a 350ºC por 1 h para separar la capa de nácar de la calcítica. Finalmente, la banda de nácar recientemente depositada es fácilmente separada del resto de la concha, al golpearla suavemente con una aguja de acero inoxidable. Éste es un método simple y rápido propuesto para el monitoreo ambiental de metales traza utilizando las conchas de M. californianus. Como un ejemplo de la aplicación de este método, se presentan los resultados de un experimento realizado en laboratorio, en el que los mejillones fueron expuestos a dos diferentes concentraciones de Cd por 60 días y sus conchas fueron procesadas por medio de esta técnica. La capa de nácar más recientemente depositada mostró incrementos significativos en sus concentraciones de Cd, los cuales fueron proporcionales al aumento en las concentraciones ambientales de exposición. |
| format | Artículo científico |
| id | redalyc_48030207 |
| institution | Redalyc Ciencias Marinas |
| language | en |
| publishDate | 2004 |
| publisher | Universidad Autónoma de Baja California |
| spelling | A technique for the separation of the most recently deposited nacreous layer in Mytilus californianus shells for trace metal analysis Técnica para la separación de la capa de nácar recientemente depositada en la concha de Mytilus californianus para el análisis de metales traza Rivero, LE Lares, ML Mytilus californianus shell nacreous layer cadmium Mytilus californianus concha capa de nácar cadmio A new method to separate a section of the most recently deposited nacreous layer from the rest of the shell of Mytilus californianus was developed. Techniques described for other mussel species proved unsuitable for this species. In this new method, the shells are soaked in 30% hydrogen peroxide for 10–15 min at 80–85ºC to eliminate the periostracum, a potential source of trace metal contamination. Next, the shells are heated in a muffle furnace at 350ºC for 1 h to separate the nacreous layer from the calcite layer. Finally, the nacreous band is easily separated from the rest of the shell by gently tapping it with a stainless steel needle. This is a simple and fast method that is proposed for trace metal monitoring purposes using M. californianus shells. To illustrate the application of the method, results from an experiment performed in our laboratory, in which mussels were exposed to two different Cd concentrations for 60 days and their shells processed by this technique, are included. The most recently deposited nacreous layer showed significant increases in Cd concentrations, which were proportional to the exposed ambient concentrations. Se desarrolló un nuevo método para separar una sección de la capa de nácar recientemente depositada del resto de la concha de Mytilus californianus. Los métodos descritos para otras especies de mejillones demostraron no ser apropiados para las conchas de esta especie. En este nuevo método, las conchas son sumergidas en peróxido de hidrógeno al 30% por 10–15 min a 80–85ºC para eliminar el periostraco, una fuente potencial de contaminación por metales traza. Enseguida, las conchas son calentadas en una mufla a 350ºC por 1 h para separar la capa de nácar de la calcítica. Finalmente, la banda de nácar recientemente depositada es fácilmente separada del resto de la concha, al golpearla suavemente con una aguja de acero inoxidable. Éste es un método simple y rápido propuesto para el monitoreo ambiental de metales traza utilizando las conchas de M. californianus. Como un ejemplo de la aplicación de este método, se presentan los resultados de un experimento realizado en laboratorio, en el que los mejillones fueron expuestos a dos diferentes concentraciones de Cd por 60 días y sus conchas fueron procesadas por medio de esta técnica. La capa de nácar más recientemente depositada mostró incrementos significativos en sus concentraciones de Cd, los cuales fueron proporcionales al aumento en las concentraciones ambientales de exposición. Universidad Autónoma de Baja California, Instituto de Investigaciones Oceanológicas 2004-03-06 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article Artículo Arbitrado application/pdf https://cienciasmarinas.com.mx/index.php/cmarinas/article/view/181 10.7773/cm.v30i2.181 Ciencias Marinas; Vol. 30 No. 2 (2004); 343-347 Ciencias Marinas; Vol. 30 Núm. 2 (2004); 343-347 2395-9053 0185-3880 eng https://cienciasmarinas.com.mx/index.php/cmarinas/article/view/181/156 ['Redalyc'] redalyc_48030207 A technique for the separation of the most recently deposited nacreous layer in Mytilus californianus shells for trace metal analysis ['A technique for the separation of the most recently deposited nacreous layer in Mytilus californianus shells for trace metal analysis', 'L. E. Rivero', 'Ciencias de la Tierra', 'shell', 'cadmium', 'nacreous layer', 'Mytilus californianus', 'A technique for the separation of the most recently deposited nacreous layer in Mytilus californianus shells for trace metal analysis L. E. Rivero M. L. Lares Ciencias de la Tierra shell cadmium nacreous layer Mytilus californianus A new method to separate a section of the most recently deposited nacreous layer from the rest of the shell of Mytiluscalifornianus was developed. Techniques described for other mussel species proved unsuitable for this species. In this newmethod, the shells are soaked in 30% hydrogen peroxide for 10\x9615 min at 80\x9685ºC to eliminate the periostracum, a potentialsource of trace metal contamination. Next, the shells are heated in a muffle furnace at 350ºC for 1 h to separate the nacreous layerfrom the calcite layer. Finally, the nacreous band is easily separated from the rest of the shell by gently tapping it with a stainlesssteel needle. This is a simple and fast method that is proposed for trace metal monitoring purposes using M. californianus shells.To illustrate the application of the method, results from an experiment performed in our laboratory, in which mussels wereexposed to two different Cd concentrations for 60 days and their shells processed by this technique, are included. The mostrecently deposited nacreous layer showed significant increases in Cd concentrations, which were proportional to the exposedambient concentrations. 2004 artículo científico 0185-3880 https://www.redalyc.org/articulo.oa?id=48030207 en http://www.redalyc.org/revista.oa?id=480 Ciencias Marinas application/pdf Universidad Autónoma de Baja California Ciencias Marinas (México) Num.2 Vol.30'] ['L. E. Rivero'] ['L. E. Rivero'] ['Artículo científico'] ['2004'] ['Universidad Autónoma de Baja California'] ['en'] ['Ciencias de la Tierra', 'shell', 'cadmium', 'nacreous layer', 'Mytilus californianus'] ['https://www.redalyc.org/articulo.oa?id=48030207'] ['A technique for the separation of the most recently deposited nacreous layer in Mytilus californianus shells for trace metal analysis L. E. Rivero M. L. Lares Ciencias de la Tierra shell cadmium nacreous layer Mytilus californianus A new method to separate a section of the most recently deposited nacreous layer from the rest of the shell of Mytiluscalifornianus was developed. Techniques described for other mussel species proved unsuitable for this species. In this newmethod, the shells are soaked in 30% hydrogen peroxide for 10\x9615 min at 80\x9685ºC to eliminate the periostracum, a potentialsource of trace metal contamination. Next, the shells are heated in a muffle furnace at 350ºC for 1 h to separate the nacreous layerfrom the calcite layer. Finally, the nacreous band is easily separated from the rest of the shell by gently tapping it with a stainlesssteel needle. This is a simple and fast method that is proposed for trace metal monitoring purposes using M. californianus shells.To illustrate the application of the method, results from an experiment performed in our laboratory, in which mussels wereexposed to two different Cd concentrations for 60 days and their shells processed by this technique, are included. The mostrecently deposited nacreous layer showed significant increases in Cd concentrations, which were proportional to the exposedambient concentrations. 2004 artículo científico 0185-3880 https://www.redalyc.org/articulo.oa?id=48030207 en http://www.redalyc.org/revista.oa?id=480 Ciencias Marinas application/pdf Universidad Autónoma de Baja California Ciencias Marinas (México) Num.2 Vol.30'] |
| spellingShingle | A technique for the separation of the most recently deposited nacreous layer in Mytilus californianus shells for trace metal analysis L. E. Rivero Ciencias de la Tierra shell cadmium nacreous layer Mytilus californianus A technique for the separation of the most recently deposited nacreous layer in Mytilus californianus shells for trace metal analysis L. E. Rivero M. L. Lares Ciencias de la Tierra shell cadmium nacreous layer Mytilus californianus A new method to separate a section of the most recently deposited nacreous layer from the rest of the shell of Mytiluscalifornianus was developed. Techniques described for other mussel species proved unsuitable for this species. In this newmethod, the shells are soaked in 30% hydrogen peroxide for 1015 min at 8085ºC to eliminate the periostracum, a potentialsource of trace metal contamination. Next, the shells are heated in a muffle furnace at 350ºC for 1 h to separate the nacreous layerfrom the calcite layer. Finally, the nacreous band is easily separated from the rest of the shell by gently tapping it with a stainlesssteel needle. This is a simple and fast method that is proposed for trace metal monitoring purposes using M. californianus shells.To illustrate the application of the method, results from an experiment performed in our laboratory, in which mussels wereexposed to two different Cd concentrations for 60 days and their shells processed by this technique, are included. The mostrecently deposited nacreous layer showed significant increases in Cd concentrations, which were proportional to the exposedambient concentrations. 2004 artículo científico 0185-3880 https://www.redalyc.org/articulo.oa?id=48030207 en http://www.redalyc.org/revista.oa?id=480 Ciencias Marinas application/pdf Universidad Autónoma de Baja California Ciencias Marinas (México) Num.2 Vol.30 |
| title | A technique for the separation of the most recently deposited nacreous layer in Mytilus californianus shells for trace metal analysis |
| title_alt | Técnica para la separación de la capa de nácar recientemente depositada en la concha de Mytilus californianus para el análisis de metales traza |
| topic | Ciencias de la Tierra shell cadmium nacreous layer Mytilus californianus |
| topic_facet | Mytilus californianus shell nacreous layer cadmium concha capa de nácar cadmio |
| url | https://www.redalyc.org/articulo.oa?id=48030207 https://cienciasmarinas.com.mx/index.php/cmarinas/article/view/181 |