Phosphatase activity in salt-ponds of the Bay of Cádiz
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| Format: | Artículo científico |
| Language: | en |
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Universidad Autónoma de Baja California
2004
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| author | M.D. Frutos |
| author_facet | M.D. Frutos |
| contents | Phosphatase activity in salt-ponds of the Bay of Cádiz M.D. Frutos A. Gómez Parra J. Blasco Ciencias de la Tierra salt ponds seawater sediment phosphatases Acid and alkaline phosphatase activities (E.C.3.1.3.2 and E.C.3.1.3.1, respectively) were measured in seawater and sedimentsfrom different sites of a salt-pond of the Bay of Cádiz. The profiles of enzymatic activity relative to depth were alsoanalysed. Initially, the optimum conditions (pH, temperature, substrate concentration and amount of sample) for determiningenzymatic activities were studied. The apparent activation energies for alkaline phosphatase activity in seawater and sedimentswere 53.8 and 48.6 kJ mol1, respectively. The Km and Vmax were 10.6 mM and 89 µg of 4-nitrophenol g1 h1 and 8.42 mM and145 µg of 4-nitrophenol L1 h1 for sediments and seawater, respectively. Both types of enzymatic activity reached high values inseawater and sediments; in the confined areas of the salt-pond the values were around 100 µg of 4-nitrophenol L1 h1 forseawater and higher for sediments. In general, clear seasonal evolutions for both types of enzymatic activity were found in theseawater, with maxima at the end of summer and minima in winter. No seasonal pattern was evident in the sediment.Stratification of phosphorus regeneration was observed in the sediment column but there was no stratification at depths greaterthan 15 cm 2004 artículo científico 0185-3880 https://www.redalyc.org/articulo.oa?id=48030303 en http://www.redalyc.org/revista.oa?id=480 Ciencias Marinas application/pdf Universidad Autónoma de Baja California Ciencias Marinas (México) Num.3 Vol.30 |
| format | Artículo científico |
| id | redalyc_48030303 |
| institution | Redalyc |
| language | en |
| publishDate | 2004 |
| publisher | Universidad Autónoma de Baja California |
| spellingShingle | Phosphatase activity in salt-ponds of the Bay of Cádiz M.D. Frutos Ciencias de la Tierra salt ponds seawater sediment phosphatases Phosphatase activity in salt-ponds of the Bay of Cádiz M.D. Frutos A. Gómez Parra J. Blasco Ciencias de la Tierra salt ponds seawater sediment phosphatases Acid and alkaline phosphatase activities (E.C.3.1.3.2 and E.C.3.1.3.1, respectively) were measured in seawater and sedimentsfrom different sites of a salt-pond of the Bay of Cádiz. The profiles of enzymatic activity relative to depth were alsoanalysed. Initially, the optimum conditions (pH, temperature, substrate concentration and amount of sample) for determiningenzymatic activities were studied. The apparent activation energies for alkaline phosphatase activity in seawater and sedimentswere 53.8 and 48.6 kJ mol1, respectively. The Km and Vmax were 10.6 mM and 89 µg of 4-nitrophenol g1 h1 and 8.42 mM and145 µg of 4-nitrophenol L1 h1 for sediments and seawater, respectively. Both types of enzymatic activity reached high values inseawater and sediments; in the confined areas of the salt-pond the values were around 100 µg of 4-nitrophenol L1 h1 forseawater and higher for sediments. In general, clear seasonal evolutions for both types of enzymatic activity were found in theseawater, with maxima at the end of summer and minima in winter. No seasonal pattern was evident in the sediment.Stratification of phosphorus regeneration was observed in the sediment column but there was no stratification at depths greaterthan 15 cm 2004 artículo científico 0185-3880 https://www.redalyc.org/articulo.oa?id=48030303 en http://www.redalyc.org/revista.oa?id=480 Ciencias Marinas application/pdf Universidad Autónoma de Baja California Ciencias Marinas (México) Num.3 Vol.30 |
| title | Phosphatase activity in salt-ponds of the Bay of Cádiz |
| topic | Ciencias de la Tierra salt ponds seawater sediment phosphatases |
| url | https://www.redalyc.org/articulo.oa?id=48030303 |