Evaluation of the estrogenic effects of UV filters on the sergeant major damselfish, Abudefduf saxatilis
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Oxybenzone, octyl salicylate, and octinoxate are compounds used in a variety of products as a protection against exposure to ultraviolet radiation. Concerns have emerged regarding their environmental safety as previous studies have shown that they tend to bioaccumulate and act as potential xenoestro...
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| Format: | Artículo científico |
| Sprache: | en |
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Universidad Autónoma de Baja California
2014
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| _version_ | 1878325300094304256 |
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| author | Mélina Soto |
| author_facet | Mélina Soto |
| collection | Ciencias Marinas |
| container_title | Ciencias Marinas |
| contents | Evaluation of the estrogenic effects of UV filters on the sergeant major damselfish, Abudefduf saxatilis Mélina Soto Gabriela Rodríguez-Fuentes Ciencias de la Tierra sunscreen vitellogenin Abudefduf saxatilis Oxybenzone, octyl salicyla te, and octinoxate are compounds used in a variet y of products as a prot ection against exposure to ultraviolet radiation. Concerns have emerged regarding their envi ronmental safety as previous st udies have shown that they tend to bioaccumulate and act as potentia l xenoestrogens in aquatic orga nisms. To our knowledge no studies have investigated their impa ct on tropical reef-associated species. Thus, the aim of the present study was to evaluate the estrogenic effects of these three compounds on the abundant, reef-associated sergea nt major damselfish, Abudefduf saxatilis . As no genomic information of A. saxatilis was available, the first part of the study was to isolate and to sequ ence the vitellogenin (VTG) and -actin genes. In the second part, the potential estrogenicity of the three compounds in juvenile fish (<5 cm) was st udied using gene expression and protein synt hesis analyses to ev aluate the induction of VTG. Fish were exposed to doses of 5, 25, and 50 g g –1 of oxybenzone, octyl salicylate, oc tinoxate, and to a mixture of them in a similar proportion to the one used in commercial personal care products. An increment in alkali-labile phosphate was observe d in all treatments, but it w as only statistically significant in the positive control (17 -estradiol). Relative gene expression was only statistically augmented in the positive control. 2014 artículo científico 0185-3880 https://www.redalyc.org/articulo.oa?id=48032071003 en http://www.redalyc.org/revista.oa?id=480 Ciencias Marinas application/pdf Universidad Autónoma de Baja California Ciencias Marinas (México) Num.3 Vol.40 |
| ctrlnum | oai:cienciasmarinas.com.mx:article/2390 10.7773/cm.v40i3.2390 |
| description | Oxybenzone, octyl salicylate, and octinoxate are compounds used in a variety of products as a protection against exposure to ultraviolet radiation. Concerns have emerged regarding their environmental safety as previous studies have shown that they tend to bioaccumulate and act as potential xenoestrogens in aquatic organisms. To our knowledge no studies have investigated their impact on tropical reef-associated species. Thus, the aim of the present study was to evaluate the estrogenic effects of these three compounds on the abundant, reef-associated sergeant major damselfish, Abudefduf saxatilis. As no genomic information of A. saxatilis was available, the first part of the study was to isolate and to sequence the vitellogenin (VTG) and β-actin genes. In the second part, the potential estrogenicity of the three compounds in juvenile fish (<5 cm) was studied using gene expression and protein synthesis analyses to evaluate the induction of VTG. Fish were exposed to doses of 5, 25, and 50 µg–1 of oxybenzone, octyl salicylate, octinoxate, and to a mixture of them in a similar proportion to the one used in commercial personal care products. An increment in alkali-labile phosphate was observed in all treatments, but it was only statistically significant in the positive control (17 β-estradiol). Relative gene expression was only statistically augmented in the positive control.
La oxibenzona, el octil salicilato y el octinoxato son compuestos empleados en una gran variedad de productos como protección contra la exposición a la radiación ultravioleta. En los últimos años ha aumentado la preocupación en cuanto a su seguridad ambiental, ya que estudios han demostrado que tienden a bioacumularse y actuar como xenoendocrinos en organismos acuáticos. A la fecha, no se han realizado estudios sobre su impacto en especies asociadas a arrecifes tropicales. Por ello, el presente trabajo investiga los efectos estrogénicos de tres filtros ultravioleta en el pez sargento, Abudefduf saxatilis. Dada la falta de información genómica disponible para A. saxatilis, una primera parte del estudio consistió en aislar y secuenciar los genes de la vitelogenina (VTG) y β-actina. En una segunda etapa, se estudiaron los efectos estrogénicos de estos tres compuestos evaluando la inducción de la expresión genética y de la síntesis de la VTG en organismos juveniles (<5 cm). Los peces se inyectaron intraperitonealmente con dosis de 5, 25 y 50 µg–1 peso húmedo de oxibenzona, octil salicilato, octinoxato y una mezcla de ellos en proporciones similares a las empleadas en las lociones comerciales. Aunque se observó un aumento en la producción de fósforo lábil al álcali en todas las muestras tratadas con los filtros UV, el incremento sólo fue estadísticamente significativo en el control positivo (17 β-estradiol). El incremento en la expresión relativa del gen fue también estadísticamente significativo únicamente en el control positivo. |
| format | Artículo científico |
| id | redalyc_48032071003 |
| institution | Redalyc Ciencias Marinas |
| language | en |
| publishDate | 2014 |
| publisher | Universidad Autónoma de Baja California |
| spelling | Evaluation of the estrogenic effects of UV filters on the sergeant major damselfish, Abudefduf saxatilis Evaluación del efecto estrogénico por filtros UV en el pez sargentoAbudefduf saxatilis Soto, Mélina Rodríguez-Fuentes, Gabriela Oxybenzone, octyl salicylate, and octinoxate are compounds used in a variety of products as a protection against exposure to ultraviolet radiation. Concerns have emerged regarding their environmental safety as previous studies have shown that they tend to bioaccumulate and act as potential xenoestrogens in aquatic organisms. To our knowledge no studies have investigated their impact on tropical reef-associated species. Thus, the aim of the present study was to evaluate the estrogenic effects of these three compounds on the abundant, reef-associated sergeant major damselfish, Abudefduf saxatilis. As no genomic information of A. saxatilis was available, the first part of the study was to isolate and to sequence the vitellogenin (VTG) and β-actin genes. In the second part, the potential estrogenicity of the three compounds in juvenile fish (<5 cm) was studied using gene expression and protein synthesis analyses to evaluate the induction of VTG. Fish were exposed to doses of 5, 25, and 50 µg–1 of oxybenzone, octyl salicylate, octinoxate, and to a mixture of them in a similar proportion to the one used in commercial personal care products. An increment in alkali-labile phosphate was observed in all treatments, but it was only statistically significant in the positive control (17 β-estradiol). Relative gene expression was only statistically augmented in the positive control. La oxibenzona, el octil salicilato y el octinoxato son compuestos empleados en una gran variedad de productos como protección contra la exposición a la radiación ultravioleta. En los últimos años ha aumentado la preocupación en cuanto a su seguridad ambiental, ya que estudios han demostrado que tienden a bioacumularse y actuar como xenoendocrinos en organismos acuáticos. A la fecha, no se han realizado estudios sobre su impacto en especies asociadas a arrecifes tropicales. Por ello, el presente trabajo investiga los efectos estrogénicos de tres filtros ultravioleta en el pez sargento, Abudefduf saxatilis. Dada la falta de información genómica disponible para A. saxatilis, una primera parte del estudio consistió en aislar y secuenciar los genes de la vitelogenina (VTG) y β-actina. En una segunda etapa, se estudiaron los efectos estrogénicos de estos tres compuestos evaluando la inducción de la expresión genética y de la síntesis de la VTG en organismos juveniles (<5 cm). Los peces se inyectaron intraperitonealmente con dosis de 5, 25 y 50 µg–1 peso húmedo de oxibenzona, octil salicilato, octinoxato y una mezcla de ellos en proporciones similares a las empleadas en las lociones comerciales. Aunque se observó un aumento en la producción de fósforo lábil al álcali en todas las muestras tratadas con los filtros UV, el incremento sólo fue estadísticamente significativo en el control positivo (17 β-estradiol). El incremento en la expresión relativa del gen fue también estadísticamente significativo únicamente en el control positivo. Universidad Autónoma de Baja California, Instituto de Investigaciones Oceanológicas 2014-09-30 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article Artículo Arbitrado application/pdf https://cienciasmarinas.com.mx/index.php/cmarinas/article/view/2390 10.7773/cm.v40i3.2390 Ciencias Marinas; Vol. 40 No. 3 (2014); 187-196 Ciencias Marinas; Vol. 40 Núm. 3 (2014); 187-196 2395-9053 0185-3880 eng https://cienciasmarinas.com.mx/index.php/cmarinas/article/view/2390/1490 ['Redalyc'] redalyc_48032071003 Evaluation of the estrogenic effects of UV filters on the sergeant major damselfish, Abudefduf saxatilis ['Evaluation of the estrogenic effects of UV filters on the sergeant major damselfish, Abudefduf saxatilis', 'Mélina Soto', 'Ciencias de la Tierra', 'sunscreen', 'vitellogenin', 'Abudefduf saxatilis', 'Evaluation of the estrogenic effects of UV filters on the sergeant major damselfish, Abudefduf saxatilis Mélina Soto Gabriela Rodríguez-Fuentes Ciencias de la Tierra sunscreen vitellogenin Abudefduf saxatilis Oxybenzone, octyl salicyla te, and octinoxate are compounds used in a variet y of products as a prot ection against exposure to ultraviolet radiation. Concerns have emerged regarding their envi ronmental safety as previous st udies have shown that they tend to bioaccumulate and act as potentia l xenoestrogens in aquatic orga nisms. To our knowledge no studies have investigated their impa ct on tropical reef-associated species. Thus, the aim of the present study was to evaluate the estrogenic effects of these three compounds on the abundant, reef-associated sergea nt major damselfish, Abudefduf saxatilis . As no genomic information of A. saxatilis was available, the first part of the study was to isolate and to sequ ence the vitellogenin (VTG) and \uf062 -actin genes. In the second part, the potential estrogenicity of the three compounds in juvenile fish (<5 cm) was st udied using gene expression and protein synt hesis analyses to ev aluate the induction of VTG. Fish were exposed to doses of 5, 25, and 50 \uf06d g g –1 of oxybenzone, octyl salicylate, oc tinoxate, and to a mixture of them in a similar proportion to the one used in commercial personal care products. An increment in alkali-labile phosphate was observe d in all treatments, but it w as only statistically significant in the positive control (17 \uf062 -estradiol). Relative gene expression was only statistically augmented in the positive control. 2014 artículo científico 0185-3880 https://www.redalyc.org/articulo.oa?id=48032071003 en http://www.redalyc.org/revista.oa?id=480 Ciencias Marinas application/pdf Universidad Autónoma de Baja California Ciencias Marinas (México) Num.3 Vol.40'] ['Mélina Soto'] ['Mélina Soto'] ['Artículo científico'] ['2014'] ['Universidad Autónoma de Baja California'] ['en'] ['Ciencias de la Tierra', 'sunscreen', 'vitellogenin', 'Abudefduf saxatilis'] ['https://www.redalyc.org/articulo.oa?id=48032071003'] ['Evaluation of the estrogenic effects of UV filters on the sergeant major damselfish, Abudefduf saxatilis Mélina Soto Gabriela Rodríguez-Fuentes Ciencias de la Tierra sunscreen vitellogenin Abudefduf saxatilis Oxybenzone, octyl salicyla te, and octinoxate are compounds used in a variet y of products as a prot ection against exposure to ultraviolet radiation. Concerns have emerged regarding their envi ronmental safety as previous st udies have shown that they tend to bioaccumulate and act as potentia l xenoestrogens in aquatic orga nisms. To our knowledge no studies have investigated their impa ct on tropical reef-associated species. Thus, the aim of the present study was to evaluate the estrogenic effects of these three compounds on the abundant, reef-associated sergea nt major damselfish, Abudefduf saxatilis . As no genomic information of A. saxatilis was available, the first part of the study was to isolate and to sequ ence the vitellogenin (VTG) and \uf062 -actin genes. In the second part, the potential estrogenicity of the three compounds in juvenile fish (<5 cm) was st udied using gene expression and protein synt hesis analyses to ev aluate the induction of VTG. Fish were exposed to doses of 5, 25, and 50 \uf06d g g –1 of oxybenzone, octyl salicylate, oc tinoxate, and to a mixture of them in a similar proportion to the one used in commercial personal care products. An increment in alkali-labile phosphate was observe d in all treatments, but it w as only statistically significant in the positive control (17 \uf062 -estradiol). Relative gene expression was only statistically augmented in the positive control. 2014 artículo científico 0185-3880 https://www.redalyc.org/articulo.oa?id=48032071003 en http://www.redalyc.org/revista.oa?id=480 Ciencias Marinas application/pdf Universidad Autónoma de Baja California Ciencias Marinas (México) Num.3 Vol.40'] |
| spellingShingle | Evaluation of the estrogenic effects of UV filters on the sergeant major damselfish, Abudefduf saxatilis Mélina Soto Ciencias de la Tierra sunscreen vitellogenin Abudefduf saxatilis Evaluation of the estrogenic effects of UV filters on the sergeant major damselfish, Abudefduf saxatilis Mélina Soto Gabriela Rodríguez-Fuentes Ciencias de la Tierra sunscreen vitellogenin Abudefduf saxatilis Oxybenzone, octyl salicyla te, and octinoxate are compounds used in a variet y of products as a prot ection against exposure to ultraviolet radiation. Concerns have emerged regarding their envi ronmental safety as previous st udies have shown that they tend to bioaccumulate and act as potentia l xenoestrogens in aquatic orga nisms. To our knowledge no studies have investigated their impa ct on tropical reef-associated species. Thus, the aim of the present study was to evaluate the estrogenic effects of these three compounds on the abundant, reef-associated sergea nt major damselfish, Abudefduf saxatilis . As no genomic information of A. saxatilis was available, the first part of the study was to isolate and to sequ ence the vitellogenin (VTG) and -actin genes. In the second part, the potential estrogenicity of the three compounds in juvenile fish (<5 cm) was st udied using gene expression and protein synt hesis analyses to ev aluate the induction of VTG. Fish were exposed to doses of 5, 25, and 50 g g –1 of oxybenzone, octyl salicylate, oc tinoxate, and to a mixture of them in a similar proportion to the one used in commercial personal care products. An increment in alkali-labile phosphate was observe d in all treatments, but it w as only statistically significant in the positive control (17 -estradiol). Relative gene expression was only statistically augmented in the positive control. 2014 artículo científico 0185-3880 https://www.redalyc.org/articulo.oa?id=48032071003 en http://www.redalyc.org/revista.oa?id=480 Ciencias Marinas application/pdf Universidad Autónoma de Baja California Ciencias Marinas (México) Num.3 Vol.40 |
| title | Evaluation of the estrogenic effects of UV filters on the sergeant major damselfish, Abudefduf saxatilis |
| title_alt | Evaluación del efecto estrogénico por filtros UV en el pez sargentoAbudefduf saxatilis |
| topic | Ciencias de la Tierra sunscreen vitellogenin Abudefduf saxatilis |
| url | https://www.redalyc.org/articulo.oa?id=48032071003 https://cienciasmarinas.com.mx/index.php/cmarinas/article/view/2390 |