Effects of cold-shock triploidy induction treatment on cell cleavage, hatching rate, and survival of loid larvae of spotted sand bass (Paralabrax maculatofasciatus)
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| Format: | Artículo científico |
| Langue: | en |
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Universidad Autónoma de Baja California
2016
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| _version_ | 1876466094616608768 |
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| author | Juan Pablo Alcántar-Vázquez |
| author_facet | Juan Pablo Alcántar-Vázquez |
| contents | Effects of cold-shock triploidy induction treatment on cell cleavage, hatching rate, and survival of loid larvae of spotted sand bass (Paralabrax maculatofasciatus) Juan Pablo Alcántar-Vázquez Silvie Dumas Renato Peña Ana M. Ibarra Ciencias de la Tierra triploidy Cell cleavage larval mortality embryoni c abnormalities Paralabrax maculatofasciatus Three experiments assessed the effects of cold-shock induced triploidy on (1) the incidence of abnormal cell cleavage during embryogenesis, (2) hatching success and the propor tion of live yolk-sac larv ae in normally cleaved versus abnormally clea ved embryos, and (3) survival of triploid larvae of spotted sand bass ( Paralabrax maculatofasciatus ). All spawns were obtaine d by hormonal induction (LHRHa). Triploidy was induced by cold shock shortly after fertilizati on. In experiment 1, the percenta ge of abnormal ce ll cleavage was significantly higher ( P < 0.05) in the cold-shocked group than in the control group, whil e the hatching rate and the prop ortion of live yolk-sac larva e were significantly lower ( P < 0.05). In experiment 2, when norm ally and abnormally cleave d embryos from the control and cold-shocked groups were incubated separa tely, normal cleaved embryos in the cold-shocked group showed a significa ntly higher hatching rate and pro portion of live yolk-sac larvae than abnorm ally cleaved embryos in the same group. No difference was observed in the control group. In exp eriment 3, a significant decrease ( P < 0.05) in the triploidy count was observed throughout the larv al period. Final survival was drastically lower for triploid larvae compared to cold-shocked diploids and untreated eggs. Low triploidy percentage s at the end of the larval period would se riously hinder the development of triploid sand bass culture. 2016 artículo científico 0185-3880 https://www.redalyc.org/articulo.oa?id=48046495003 en http://www.redalyc.org/revista.oa?id=480 Ciencias Marinas application/pdf Universidad Autónoma de Baja California Ciencias Marinas (México) Num.2 Vol.42 |
| format | Artículo científico |
| id | redalyc_48046495003 |
| institution | Redalyc |
| language | en |
| publishDate | 2016 |
| publisher | Universidad Autónoma de Baja California |
| spellingShingle | Effects of cold-shock triploidy induction treatment on cell cleavage, hatching rate, and survival of loid larvae of spotted sand bass (Paralabrax maculatofasciatus) Juan Pablo Alcántar-Vázquez Ciencias de la Tierra triploidy Cell cleavage larval mortality embryoni c abnormalities Paralabrax maculatofasciatus Effects of cold-shock triploidy induction treatment on cell cleavage, hatching rate, and survival of loid larvae of spotted sand bass (Paralabrax maculatofasciatus) Juan Pablo Alcántar-Vázquez Silvie Dumas Renato Peña Ana M. Ibarra Ciencias de la Tierra triploidy Cell cleavage larval mortality embryoni c abnormalities Paralabrax maculatofasciatus Three experiments assessed the effects of cold-shock induced triploidy on (1) the incidence of abnormal cell cleavage during embryogenesis, (2) hatching success and the propor tion of live yolk-sac larv ae in normally cleaved versus abnormally clea ved embryos, and (3) survival of triploid larvae of spotted sand bass ( Paralabrax maculatofasciatus ). All spawns were obtaine d by hormonal induction (LHRHa). Triploidy was induced by cold shock shortly after fertilizati on. In experiment 1, the percenta ge of abnormal ce ll cleavage was significantly higher ( P < 0.05) in the cold-shocked group than in the control group, whil e the hatching rate and the prop ortion of live yolk-sac larva e were significantly lower ( P < 0.05). In experiment 2, when norm ally and abnormally cleave d embryos from the control and cold-shocked groups were incubated separa tely, normal cleaved embryos in the cold-shocked group showed a significa ntly higher hatching rate and pro portion of live yolk-sac larvae than abnorm ally cleaved embryos in the same group. No difference was observed in the control group. In exp eriment 3, a significant decrease ( P < 0.05) in the triploidy count was observed throughout the larv al period. Final survival was drastically lower for triploid larvae compared to cold-shocked diploids and untreated eggs. Low triploidy percentage s at the end of the larval period would se riously hinder the development of triploid sand bass culture. 2016 artículo científico 0185-3880 https://www.redalyc.org/articulo.oa?id=48046495003 en http://www.redalyc.org/revista.oa?id=480 Ciencias Marinas application/pdf Universidad Autónoma de Baja California Ciencias Marinas (México) Num.2 Vol.42 |
| title | Effects of cold-shock triploidy induction treatment on cell cleavage, hatching rate, and survival of loid larvae of spotted sand bass (Paralabrax maculatofasciatus) |
| topic | Ciencias de la Tierra triploidy Cell cleavage larval mortality embryoni c abnormalities Paralabrax maculatofasciatus |
| url | https://www.redalyc.org/articulo.oa?id=48046495003 |