OAK WOOD INHABITING FUNGI AND THEIR EFFECT ON LIGNIN STUDIED BY UV MICROSPECTROPHOTOMETRY

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1. Verfasser: Leila Karami
Format: Artículo científico
Sprache:en
Veröffentlicht: Universidad del Bío Bío 2014
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author Leila Karami
author_facet Leila Karami
contents OAK WOOD INHABITING FUNGI AND THEIR EFFECT ON LIGNIN STUDIED BY UV MICROSPECTROPHOTOMETRY Leila Karami Jörg Fromm Gerald Koch Olaf Schmidt Uwe Schmitt Agrociencias UV rDNA fungi ITS sequencing lignin content Decayed-wood samples were collected from a naturally-infected bridge made of Quercus robur. Fruiting bodies of the white-rot basidiomycetes Hymenochaete rubiginosa and Stereum hirsutum were sampled. The white-rot fungus Fuscoporia ferrea and the secondary saprobiont Mycena galericulata were identified from the rotten wood by means of rDNA-ITS sequencing. The topochemistry of lignin degradation within individual cell-wall layers was determined by cellular UV-microspectrophotometry (UMSP) at 278 nm wavelength. Increased delignification occurred in compound middle lamella regions (CML), secondary wall layers of fibres (S2), longitudinal and ray parenchyma cells as well in vessels. The highest lignin content at initial and medium decay was recorded in the CML, whereas in advanced decay secondary wall layers of the vessels exhibited the highest content of residual lignin. In all stages of degradation, the S2 layers of fibres had the lowest lignin amount. 2014 artículo científico 0717-3644 https://www.redalyc.org/articulo.oa?id=48530204002 en http://www.redalyc.org/revista.oa?id=485 Maderas. Ciencia y Tecnología application/pdf Universidad del Bío Bío Maderas. Ciencia y Tecnología (Chile) Num.2 Vol.16
format Artículo científico
id redalyc_48530204002
institution Redalyc
language en
publishDate 2014
publisher Universidad del Bío Bío
spellingShingle OAK WOOD INHABITING FUNGI AND THEIR EFFECT ON LIGNIN STUDIED BY UV MICROSPECTROPHOTOMETRY
Leila Karami
Agrociencias
UV
rDNA
fungi
ITS sequencing
lignin content
OAK WOOD INHABITING FUNGI AND THEIR EFFECT ON LIGNIN STUDIED BY UV MICROSPECTROPHOTOMETRY Leila Karami Jörg Fromm Gerald Koch Olaf Schmidt Uwe Schmitt Agrociencias UV rDNA fungi ITS sequencing lignin content Decayed-wood samples were collected from a naturally-infected bridge made of Quercus robur. Fruiting bodies of the white-rot basidiomycetes Hymenochaete rubiginosa and Stereum hirsutum were sampled. The white-rot fungus Fuscoporia ferrea and the secondary saprobiont Mycena galericulata were identified from the rotten wood by means of rDNA-ITS sequencing. The topochemistry of lignin degradation within individual cell-wall layers was determined by cellular UV-microspectrophotometry (UMSP) at 278 nm wavelength. Increased delignification occurred in compound middle lamella regions (CML), secondary wall layers of fibres (S2), longitudinal and ray parenchyma cells as well in vessels. The highest lignin content at initial and medium decay was recorded in the CML, whereas in advanced decay secondary wall layers of the vessels exhibited the highest content of residual lignin. In all stages of degradation, the S2 layers of fibres had the lowest lignin amount. 2014 artículo científico 0717-3644 https://www.redalyc.org/articulo.oa?id=48530204002 en http://www.redalyc.org/revista.oa?id=485 Maderas. Ciencia y Tecnología application/pdf Universidad del Bío Bío Maderas. Ciencia y Tecnología (Chile) Num.2 Vol.16
title OAK WOOD INHABITING FUNGI AND THEIR EFFECT ON LIGNIN STUDIED BY UV MICROSPECTROPHOTOMETRY
topic Agrociencias
UV
rDNA
fungi
ITS sequencing
lignin content
url https://www.redalyc.org/articulo.oa?id=48530204002